PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1469 [new locus tag: SAUSA300_RS08020 ]
• pan locus tag^?: SAUPAN004075000
• symbol: argR
• pan gene symbol^?: argR
• synonym: ahrC
• product: arginine repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1469 [new locus tag: SAUSA300_RS08020 ]
• symbol: argR
• product: arginine repressor
• replicon: chromosome
• strand: -
• coordinates: 1624622..1625074
• length: 453
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913347 NCBI
• RefSeq: YP_494165 NCBI
• BioCyc: see SAUSA300_RS08020
• MicrobesOnline: 1292984 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  GTGCCCAAAAAATCGGTTAGGCATATAAAAATTAGAGAAATTATTTCAAATGAACAGATA
  GAGACACAAGATGAATTAGTTAAACGATTAAACGATTATGATTTAAATGTCACTCAAGCA
  ACTGTTTCTCGTGATATTAAAGAACTACAACTTATTAAAGTACCTATACCTTCAGGTCAA
  TATGTTTATAGTTTACCAAATGATAGAAAATTCCATCCTTTAGAAAAATTGGGACGTTAT
  TTAATGGATTCCTTTGTTAATATAGATGGTACTGATAATTTACTTGTTCTAAAAACATTA
  CCTGGTAATGCACAATCTATTGGAGCTATATTAGACCAAATCAATTGGGAAGAAGTACTA
  GGCACAATTTGTGGTGATGATACTTGTTTAATTATTTGTCGAAGCAAAGAGGCAAGTGAT
  GAAATCAAGTCAAGAATTTTCAATTTGTTATAA
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  453
  

⊟Protein

⊟General

• locus tag: SAUSA300_1469 [new locus tag: SAUSA300_RS08020 ]
• symbol: ArgR
• description: arginine repressor
• length: 150
• theoretical pI: 5.28159
• theoretical MW: 17097.6
• GRAVY: -0.267333

⊟Function

• TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family arginine repressor (TIGR01529; HMM-score: 169.8)
  Signal transduction Regulatory functions DNA interactions arginine repressor (TIGR01529; HMM-score: 169.8)
• TheSEED  :
  • Arginine pathway regulatory protein ArgR, repressor of arg regulon
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Arginine pathway regulatory protein ArgR, repressor of arg regulon
  and 2 more
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Arginine pathway regulatory protein ArgR, repressor of arg regulon
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Arginine pathway regulatory protein ArgR, repressor of arg regulon
• PFAM:
  HTH (CL0123) Arg_repressor; Arginine repressor, DNA binding domain (PF01316; HMM-score: 94.5)
  no clan defined Arg_repressor_C; Arginine repressor, C-terminal domain (PF02863; HMM-score: 92.4)
  and 1 more
  HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 17)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effector: Arginine
• genes regulated by ArgR/AhrC, TF important in Arginine biosynthesis, Arginine degradationRegPrecise

  repression

  arcD < arcA

  argB < argJ < argC < rocD

  rocD

  argH < argG

  recN < argR

  glnQ* < glnP*

  bccT

  arcR < arcC < arcD < arcB < arcA

  transcription units transferred from N315 data RegPrecise

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003318
  • TAT(Tat/SPI): 0.000875
  • LIPO(Sec/SPII): 0.000397
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160026 NCBI
• RefSeq: YP_494165 NCBI
• UniProt: Q2FGL3 UniProt

⊟Protein sequence

• MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSRDIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPGNAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: recN < argR

⊟Regulation

• regulator: ArgR/AhrC (repression) regulon

  ArgR/AhrC

  (TF)

  important in Arginine biosynthesis, Arginine degradation; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications