PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0065 [new locus tag: SAUSA300_RS00330 ]
• pan locus tag^?: SAUPAN000814000
• symbol: arcA
• pan gene symbol^?: arcA
• synonym:
• product: arginine deiminase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0065 [new locus tag: SAUSA300_RS00330 ]
• symbol: arcA
• product: arginine deiminase
• replicon: chromosome
• strand: -
• coordinates: 73113..74348
• length: 1236
• essential: unknown

⊟Accession numbers

• Gene ID: 3914803 NCBI
• RefSeq: YP_492784 NCBI
• BioCyc: see SAUSA300_RS00330
• MicrobesOnline: 1291580 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGTACAAGGACCCATTCAAGTAAATAGTGAAATAGGCAAATTGAAAACTGTGTTGTTA
  AAAAGACCAGGAAAAGAATTAGAAAATTTAGTACCTGATCATTTAAGTGGTTTATTATTC
  GATGATATTCCCTACTTAAAAGTTGCACAAGAAGAGCATGACAAATTTGCTCAAACTTTG
  AGAGATGAAGGAATCGAAGTAGTTTATTTAGAAAAACTTGCAGCAGAATCTATTACTGAG
  CCAGAAGTACGCGAGAACTTCATAAACGACATATTAACAGAATCTAAAAAGACAATATTA
  GGTCATGAAACTGAAATTAAAGAATTCTTTTCAAAGTTATCTGACCAAGAACTTGTAAAT
  AAAATCATGGCTGGCGTACGTAAAGAAGAAATTCAACTTGAAACAACCCATTTAGTAGAA
  TATATGGATGATAGATATCCATTTTACTTAGATCCAATGCCCAACCTTTATTTTACAAGA
  GATCCCCAAGCTTCAATTGGTAGAGGAATGACAATTAACAGAATGTATTGGAGAGCACGA
  CGTAGAGAATCTATTTTTATGACATATATACTGAAACATCATCCAAGATTTAAAGATAAA
  GATGTACCAGTATGGTTAGATCGTAACTCACCATTTAATATTGAAGGTGGAGATGAATTA
  GTATTATCGAAAGATGTTTTAGCTATTGGTATATCAGAACGTACATCAGCTCAAGCAATA
  GAAAAGTTAGCACGTAATATTTTCAAAGATGCAAACACAAGTTTTAAAAAAATCGTAGCT
  ATTGAAATACCTAATACACGTACATTTATGCACCTAGATACAGTACTAACTATGATTGAC
  TACGATAAGTTTACAGTACATGCAGCAATATTTAAAGAAGAAAATAATATGAATATATTT
  ACCATAGAACAAAATGATGGTAAGGACGATATAAAAATTACTCGTTCTAGCAAGTTACGT
  GAAACACTTGCTGAAGTTTTAGAAGTAGAAAAAGTGGACTTTATTCCAACAGGTAATGGC
  GACGTTATTGATGGTGCACGTGAACAATGGAATGATGGCTCAAACACATTATGTATTCGA
  CCAGGGGTTGTGGTGACATACGATCGCAACTATGTATCAAACCAACTTTTACGCGACAAA
  GGAATTAAAGTGATTGAAATTACTGGTAGTGAACTTGTACGTGGACGCGGAGGCCCAAGA
  TGTATGAGTCAGCCGTTATTTAGAGAAGATATTTAA
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  1236
  

⊟Protein

⊟General

• locus tag: SAUSA300_0065 [new locus tag: SAUSA300_RS00330 ]
• symbol: ArcA
• description: arginine deiminase
• length: 411
• theoretical pI: 5.18702
• theoretical MW: 47270.7
• GRAVY: -0.42944

⊟Function

• reaction:
  EC 3.5.3.6?  ExPASy
  Arginine deiminase L-arginine + H₂O = L-citrulline + NH₃
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines arginine deiminase (TIGR01078; EC 3.5.3.6; HMM-score: 558.9)
  and 1 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 12.5)
• TheSEED  :
  • Arginine deiminase (EC 3.5.3.6)
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Arginine deiminase (EC 3.5.3.6)
  and 1 more
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Arginine deiminase (EC 3.5.3.6)
• PFAM:
  GME (CL0197) Amidinotransf; Amidinotransferase (PF02274; HMM-score: 390.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001251
  • TAT(Tat/SPI): 0.000092
  • LIPO(Sec/SPII): 0.000202
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161307 NCBI
• RefSeq: YP_492784 NCBI
• UniProt: A0A0H2XGY3 UniProt

⊟Protein sequence

• MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIPYLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGHETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTRDPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGDELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVLTMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDFIPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSELVRGRGGPRCMSQPLFREDI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: arcC < arcB < SAUSA300_0063 < arcD < arcA

⊟Regulation

• regulators: ArcR (activation) regulon, ArgR/AhrC (repression) regulon, CcpA regulon, Rex (repression) regulon

  ArcR	(TF)	important in Arginine degradation; RegPrecise
  ArgR/AhrC	(TF)	important in Arginine biosynthesis, Arginine degradation; RegPrecise
  CcpA	(TF)	important in Carbon catabolism; RegPrecise
  Rex	(TF)	important in Energy metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications