PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0186 [new locus tag: SAUSA300_RS00975 ]
• pan locus tag^?: SAUPAN001024000
• symbol: argC
• pan gene symbol^?: argC
• synonym:
• product: N-acetyl-gamma-glutamyl-phosphate reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0186 [new locus tag: SAUSA300_RS00975 ]
• symbol: argC
• product: N-acetyl-gamma-glutamyl-phosphate reductase
• replicon: chromosome
• strand: -
• coordinates: 214657..215688
• length: 1032
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913575 NCBI
• RefSeq: YP_492900 NCBI
• BioCyc: see SAUSA300_RS00975
• MicrobesOnline: 1291701 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  ATGATTAAAGTAGGTATCGTTGGCGGTAGCGGTTATGGCGCAATTGAATTAATTCGATTG
  TTACAAACACATCCTCATGTAACGATTGCACACATCTACTCACATTCAAAAGTAGATGAA
  CCGTTGAAATTAACATTTCCACATTTACAACATATTATGCAACATTTCGAAGCACTTACA
  GTGGACAATAATGACTGTGATGTAATTTTCTTTGCGACACCAGCACCCGTAAGTAAAACA
  TGTATCCCTCCCTTAGTAGAAAAAGGTATTCATGTTATCGATTTATCTGGCGCATTTAGA
  ATTAAGAATCGTGAAATATATGAAGCATATTACAAAGAAACTGCTGCAGCACAAGATGAT
  TTGAATCATGCTATTTACAGCATTTCAGAATGGCAATCGTTTGATAACAATGGAACGAAG
  CTCATTTCTAATCCTGGCTGTTTCCCTACAGCAACATTATTAGCATTACATCCACTTATT
  AGCGAAAAAATAGTAGATTTGTCATCTATTATTATTGATGCTAAGACCGGCGTGTCAGGT
  GCTGGTCGTTCATTATCACAACGTGTTCATTTTTCAGAAATGAATGAAAATCTAAGCGCT
  TATGCAATCGGAAACCATAAACACAAACCGGAAATCGAGCAATATTTATCTATCATTGCG
  GGTCAAGATGTATCAGTCATATTTACACCACATCTCGTACCAATGACACGAGGTATTTTA
  TCAACAATATATGTCAAATTATCATCTGAATATACGACTGAATCATTACATAAATTAATG
  ACCTCTTATTATGCTAATCAGCCATTTGTCAGAATTAGAGATATTGGGACTTTTCCAACC
  ACAAAAGAAGTACTCGGTAGTAACTACTGCGATATCGGCATCTATGTAGATGAAACAACG
  CAAACAGCAATTTTAGTATCAGTGATTGATAACCTTGTCAAAGGCGCAAGTGGGCAAGCC
  ATTCAAAATTTAAATATATTATATGATTTTGAAGTGACGACTGGCCTAAATCAATCACCA
  GTTTATCCATAA
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  1032
  

⊟Protein

⊟General

• locus tag: SAUSA300_0186 [new locus tag: SAUSA300_RS00975 ]
• symbol: ArgC
• description: N-acetyl-gamma-glutamyl-phosphate reductase
• length: 343
• theoretical pI: 6.22283
• theoretical MW: 37890
• GRAVY: 0.0157434

⊟Function

• reaction:
  EC 1.2.1.38?  ExPASy
  N-acetyl-gamma-glutamyl-phosphate reductase N-acetyl-L-glutamate 5-semialdehyde + NADP⁺ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family N-acetyl-gamma-glutamyl-phosphate reductase (TIGR01850; EC 1.2.1.38; HMM-score: 440.3)
  and 4 more
  Metabolism Amino acid biosynthesis Glutamate family N-acetyl-gamma-glutamyl-phosphate reductase (TIGR01851; EC 1.2.1.38; HMM-score: 99.6)
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR00978; EC 1.2.1.11; HMM-score: 59.4)
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 45.7)
  acetaldehyde dehydrogenase (acetylating) (TIGR03215; EC 1.2.1.10; HMM-score: 13)
• TheSEED  :
  • N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38)
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38)
• PFAM:
  GADPH_aa-bio_dh (CL0139) Semialdhyde_dhC; Semialdehyde dehydrogenase, dimerisation domain (PF02774; HMM-score: 99.6)
  NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 91.6)
  and 1 more
  GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 15.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006392
  • TAT(Tat/SPI): 0.000302
  • LIPO(Sec/SPII): 0.0007
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161009 NCBI
• RefSeq: YP_492900 NCBI
• UniProt: Q2FK78 UniProt

⊟Protein sequence

• MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKLTFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIKNREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLISEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSIIAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIGTFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTTGLNQSPVYP

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: argB < argJ < argC < rocD

⊟Regulation

• regulator: ArgR/AhrC (repression) regulon

  ArgR/AhrC

  (TF)

  important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications