COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 27-JUN-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SAP025 [new locus tag: SA_RS00115 ]
• pan locus tag^?:
• symbol: SAP025
• pan gene symbol^?: —
• synonym:
• product: truncated Pre protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAP025 [new locus tag: SA_RS00115 ]
• symbol: SAP025
• product: truncated Pre protein
• replicon: pN315
• strand: +
• coordinates: 20652..21206
• length: 555
• essential: unknown

⊟Accession numbers

• Gene ID: 1122753 NCBI
• RefSeq: NP_395561 NCBI
• BioCyc: see SA_RS00115
• MicrobesOnline: 143495 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGATACAAAACTACCAAAAATCATTAGATACGCTTAAAAAGCTTCTGAGTGTCATGTAT
  GAAATTAAAACTAAAAATGTAGGTGGATGGTTTCATAAAGAGAAGCAAGAAACAGGCAAT
  ATTGTAATTACTAAAACTTATTTTGAAAAATACACTAAACAAATTAAAGCAGCACAGATG
  ATATTAGATGATTATGAATGGATTAAAAGTGGGAAGTCATTAAAAAAATCAGAGAAACAA
  AATGAAAGTTTAGTTAATGAATTGACTAGTGTCCATATGGAAAATGAAAAGCTGGTTGAA
  GAATTTAATGATTTAGCACAACGTTATAATTATTTGTTGTCAGAGAATGAAAAGAAAGAT
  AAAGAACTAAATTATACGCTTAAGTTATTCAATCAAGTTTTTAAAATCATTAAAAGTATG
  ATGAAAGAAGAGCGTTATCATACGCTAATAAATCATATTGATAATCATTTAGACAATTCT
  AAAATACGTGAAGTAATGACGATAGATAACAATGATGAACAGTTCTTTAAGAAGAAATAT
  CAAGCACAAGAATGA
  60
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  420
  480
  540
  555
  

⊟Protein

⊟General

• locus tag: SAP025 [new locus tag: SA_RS00115 ]
• symbol: SAP025
• description: truncated Pre protein
• length: 184
• theoretical pI: 9.04015
• theoretical MW: 22149.2
• GRAVY: -0.920109

⊟Function

• TIGRFAM:
• TheSEED  :
  • truncated Pre protein
• PFAM:
  TPR (CL0020) TPR_AP5Z1; AP-5 complex subunit zeta-1, TPR repeats (PF25153; HMM-score: 17.4)
  FtsL (CL0225) ZapB; Cell division protein ZapB (PF06005; HMM-score: 15.3)
  and 6 more
  no clan defined MTR4-like_stalk; Exosome RNA helicase MTR4-like, stalk (PF21408; HMM-score: 13.6)
  GST_C (CL0497) Arc1p_N_like; Nuclear-export cofactor Arc1p (PF21972; HMM-score: 13.6)
  Spectrin (CL0743) Spectrin; Spectrin repeat (PF00435; HMM-score: 12.6)
  Kinetochore (CL0724) Spc24; Spc24 subunit of Ndc80 (PF08286; HMM-score: 11.4)
  no clan defined APG6_N; Apg6 coiled-coil region (PF17675; HMM-score: 9.6)
  DMPK_coil; DMPK coiled coil domain like (PF08826; HMM-score: 8.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8458
  • Cytoplasmic Membrane Score: 0.0382
  • Cell wall & surface Score: 0.0013
  • Extracellular Score: 0.1147
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005409
  • TAT(Tat/SPI): 0.000235
  • LIPO(Sec/SPII): 0.001509
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 16119225 NCBI
• RefSeq: NP_395561 NCBI
• UniProt: A0A0H3JWK9 UniProt

⊟Protein sequence

• MIQNYQKSLDTLKKLLSVMYEIKTKNVGGWFHKEKQETGNIVITKTYFEKYTKQIKAAQMILDDYEWIKSGKSLKKSEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQAQE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications