COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00115 [old locus tag: SAP025 ]
• pan locus tag^?:
• symbol: SA_RS00115
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00115 [old locus tag: SAP025 ]
• symbol: SA_RS00115
• product: hypothetical protein
• replicon: pN315
• strand: +
• coordinates: 20706..21206
• length: 501
• essential: unknown

⊟Accession numbers

• Location: NC_003140 (20706..21206) NCBI
• BioCyc: SA_RS00115 BioCyc
• MicrobesOnline: see SAP025

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGTATGAAATTAAAACTAAAAATGTAGGTGGATGGTTTCATAAAGAGAAGCAAGAAACA
  GGCAATATTGTAATTACTAAAACTTATTTTGAAAAATACACTAAACAAATTAAAGCAGCA
  CAGATGATATTAGATGATTATGAATGGATTAAAAGTGGGAAGTCATTAAAAAAATCAGAG
  AAACAAAATGAAAGTTTAGTTAATGAATTGACTAGTGTCCATATGGAAAATGAAAAGCTG
  GTTGAAGAATTTAATGATTTAGCACAACGTTATAATTATTTGTTGTCAGAGAATGAAAAG
  AAAGATAAAGAACTAAATTATACGCTTAAGTTATTCAATCAAGTTTTTAAAATCATTAAA
  AGTATGATGAAAGAAGAGCGTTATCATACGCTAATAAATCATATTGATAATCATTTAGAC
  AATTCTAAAATACGTGAAGTAATGACGATAGATAACAATGATGAACAGTTCTTTAAGAAG
  AAATATCAAGCACAAGAATGA
  60
  120
  180
  240
  300
  360
  420
  480
  501
  

⊟Protein

⊟General

• locus tag: SA_RS00115 [old locus tag: SAP025 ]
• symbol: SA_RS00115
• description: hypothetical protein
• length: 166
• theoretical pI: 7.83841
• theoretical MW: 20044.7
• GRAVY: -0.998795

⊟Function

• TIGRFAM:
• TheSEED: see SAP025
• PFAM:
  TPR (CL0020) TPR_AP5Z1; AP-5 complex subunit zeta-1, TPR repeats (PF25153; HMM-score: 17.1)
  FtsL (CL0225) ZapB; Cell division protein ZapB (PF06005; HMM-score: 16.4)
  Spectrin (CL0743) Spectrin; Spectrin repeat (PF00435; HMM-score: 13.9)
  no clan defined MTR4-like_stalk; Exosome RNA helicase MTR4-like, stalk (PF21408; HMM-score: 13.8)
  APG6_N; Apg6 coiled-coil region (PF17675; HMM-score: 13.7)
  and 3 more
  GST_C (CL0497) Arc1p_N_like; Nuclear-export cofactor Arc1p (PF21972; HMM-score: 13.6)
  Kinetochore (CL0724) Spc24; Spc24 subunit of Ndc80 (PF08286; HMM-score: 13)
  no clan defined DMPK_coil; DMPK coiled coil domain like (PF08826; HMM-score: 8.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7989
  • Cytoplasmic Membrane Score: 0.0011
  • Cell wall & surface Score: 0.0017
  • Extracellular Score: 0.1983
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004273
  • TAT(Tat/SPI): 0.000176
  • LIPO(Sec/SPII): 0.000388
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446195152 NCBI
• RefSeq: WP_000273007 NCBI
• UniProt: see SAP025

⊟Protein sequence

• MYEIKTKNVGGWFHKEKQETGNIVITKTYFEKYTKQIKAAQMILDDYEWIKSGKSLKKSEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQAQE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications