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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SAS031 [new locus tag: SA_RS05425 ]
  • pan locus tag?: SAUPAN003335000
  • symbol: SAS031
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAS031 [new locus tag: SA_RS05425 ]
  • symbol: SAS031
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1085801..1085992
  • length: 192
  • essential: no DEG

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    ATGAAACAAAAAAAATCTAAAAATATCTTTTGGGTATTTTCTATATTAGCTGTTGTTTTC
    TTAGTATTATTTAGTTTTGCTGTTGGTGCATCAAATGTACCAATGATGATTTTAACATTT
    ATATTACTCGTTGCAACCTTTGGAATTGGATTTACTACAAAGAAAAAATATCGAGAAAAC
    GATTGGCTATAA
    60
    120
    180
    192

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAS031 [new locus tag: SA_RS05425 ]
  • symbol: SAS031
  • description: hypothetical protein
  • length: 63
  • theoretical pI: 10.9505
  • theoretical MW: 7249.77
  • GRAVY: 0.779365

Function[edit | edit source]

  • TIGRFAM:
    early transcribed membrane protein (ETRAMP) family (TIGR01495; HMM-score: 14.6)
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 12.7)
    cxxc_20_cxxc protein (TIGR04104; HMM-score: 12.5)
    and 1 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EssA (TIGR03927; HMM-score: 6.5)
  • TheSEED  :
    • FIG01107843: hypothetical protein
  • PFAM:
    no clan defined DUF5325; Family of unknown function (DUF5325) (PF17259; HMM-score: 72.2)
    and 14 more
    DUF3792; Protein of unknown function (DUF3792) (PF12670; HMM-score: 18.8)
    DUF4231; Protein of unknown function (DUF4231) (PF14015; HMM-score: 17)
    DUF4181; Domain of unknown function (DUF4181) (PF13789; HMM-score: 16.6)
    DUF4133; Domain of unknown function (DUF4133) (PF13571; HMM-score: 14.8)
    DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 14.1)
    DUF5392; Family of unknown function (DUF5392) (PF17370; HMM-score: 14)
    Hypoth_1 (CL0447) DUF3784; Domain of unknown function (DUF3784) (PF12650; HMM-score: 13.5)
    no clan defined DUF3754; Protein of unknown function (DUF3754) (PF12576; HMM-score: 13.1)
    DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 13)
    ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 12.9)
    PHO4; Phosphate transporter family (PF01384; HMM-score: 11.8)
    PBP_N; Penicillin-binding protein N-terminus (PF17093; HMM-score: 11.6)
    SNARE; SNARE domain (PF05739; HMM-score: 10.2)
    Halogen_Hydrol; 5-bromo-4-chloroindolyl phosphate hydrolysis protein (PF10112; HMM-score: 8.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.078075
    • TAT(Tat/SPI): 0.005197
    • LIPO(Sec/SPII): 0.052512
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLVATFGIGFTTKKKYRENDWL

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]