From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

Summary[edit | edit source]

  • pan ID?: SAUPAN001397000
  • symbol?: recT
  • synonym:
  • description?: recombinase

      descriptions from strain specific annotations:

    • recombinase
    • putative phage-related DNA recombination protein
    • phage protein
    • Recombinational DNA repair protein RecT
    • recombination protein RecT
    • RecT protein
  • strand?: +
  • coordinates?: 1680714..1681634
  • synteny block?: BlockID0008760
  • occurrence?: in 24% of 34 strains

rec (recT) : prophage ssDNA recombinase [1]

Single-stranded DNA recombinases (alt: single-stranded DNA annealing proteins [SAPs]) recombine complementary dsDNA sequences that contain 3'-ssDNA overhangs. Several paralogues exist - this version is RecT-like but other versions in staphylococcal phages can include Sak4, Sak, Erf and DUF2800. Prophage SAPs are typically paired with a single-strand DNA exonuclease and a single-strand DNA-binding protein.

Orthologs[edit | edit source]

    COL:
    N315:
    NCTC8325:
    Newman:
    USA300_FPR3757:
    04-02981:
    08BA02176:
    11819-97:
    6850:
    71193:
    ECT-R 2:
    ED133:
    ED98:
    HO 5096 0412:
    JH1:
    SaurJH1_0329
    JH9:
    SaurJH9_0320
    JKD6008:
    SAA6008_02040 (recT)
    JKD6159:
    JSNZ:
    LGA251:
    M013:
    MRSA252:
    MSHR1132:
    MSSA476:
    Mu3:
    Mu50:
    MW2:
    RF122:
    ST398:
    T0131:
    SAT0131_02121
    TCH60:
    TW20:
    SATW20_03400
    USA300_TCH1516:
    USA300HOU_1991 (recT)
    VC40:

Genome Viewer[edit | edit source]

Newman
USA300_FPR3757

Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.307)


    Newman          MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYSPSNAMKQAWLQISQDN
    USA300_FPR3757  MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYSPSNAMKQAWLQISQDN
                    ************************************************************

    Newman          KLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQRSYHGNVMMLKRDAGAQDV
    USA300_FPR3757  KLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQRSYHGNVMMLKRDAGAQDV
                    ************************************************************

    Newman          VAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIGAYCTIVFNDGRDNYIEVMTI
    USA300_FPR3757  VAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIGAYCTIVFNDGRDNYIEVMTI
                    ************************************************************

    Newman          EQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVINRAAKRYINTSTDSNIFKYAQE
    USA300_FPR3757  EQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVINRAAKRYINTSTDSNLFKYAQE
                    *****************************************************:******

    Newman          SEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYTELENDKPIDVSDFEEIKEPATEKE
    USA300_FPR3757  SEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYTELENDKPIDVSDFEEIKEPATEKE
                    ************************************************************

    Newman          SEEEPF
    USA300_FPR3757  SEEEPF
                    ******

  1. J P Muyrers, Y Zhang, F Buchholz, A F Stewart
    RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners.
    Genes Dev: 2000, 14(15);1971-82
    [PubMed:10921910] [WorldCat.org] (P p)
    Lakshminarayan M Iyer, Eugene V Koonin, L Aravind
    Classification and evolutionary history of the single-strand annealing proteins, RecT, Redbeta, ERF and RAD52.
    BMC Genomics: 2002, 3;8
    [PubMed:11914131] [WorldCat.org] [DOI] (P p)
    Simanti Datta, Nina Costantino, Xiaomei Zhou, Donald L Court
    Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages.
    Proc Natl Acad Sci U S A: 2008, 105(5);1626-31
    [PubMed:18230724] [WorldCat.org] [DOI] (I p)
    Maan M Neamah, Ignacio Mir-Sanchis, María López-Sanz, Sonia Acosta, Ignacio Baquedano, Andreas F Haag, Alberto Marina, Silvia Ayora, José R Penadés
    Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus.
    Nucleic Acids Res: 2017, 45(11);6507-6519
    [PubMed:28475766] [WorldCat.org] [DOI] (I p)
    Janine Bowring, Maan M Neamah, Jorge Donderis, Ignacio Mir-Sanchis, Christian Alite, J Rafael Ciges-Tomas, Elisa Maiques, Iltyar Medmedov, Alberto Marina, José R Penadés
    Pirating conserved phage mechanisms promotes promiscuous staphylococcal pathogenicity island transfer.
    Elife: 2017, 6;
    [PubMed:28826473] [WorldCat.org] [DOI] (I e)
    Kamil Steczkiewicz, Eric Prestel, Elena Bidnenko, Agnieszka K Szczepankowska
    Expanding Diversity of Firmicutes Single-Strand Annealing Proteins: A Putative Role of Bacteriophage-Host Arms Race.
    Front Microbiol: 2021, 12;644622
    [PubMed:33959107] [WorldCat.org] [DOI] (P e)
    Chrispin Chaguza, Joshua T Smith, Spencer A Bruce, Robert Gibson, Isabella W Martin, Cheryl P Andam
    Prophage-encoded immune evasion factors are critical for Staphylococcus aureus host infection, switching, and adaptation.
    Cell Genom: 2022, 2(11);
    [PubMed:36465278] [WorldCat.org] [DOI] (I p)