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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
⊟Summary[edit | edit source]
- pan ID?: SAUPAN001397000
- symbol?: recT
- synonym:
- description?: recombinase
- recombinase
- putative phage-related DNA recombination protein
- phage protein
- Recombinational DNA repair protein RecT
- recombination protein RecT
- RecT protein
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 1680714..1681634
- synteny block?: BlockID0008760
- occurrence?: in 24% of 34 strains
rec (recT) : prophage ssDNA recombinase [1]
Single-stranded DNA recombinases (alt: single-stranded DNA annealing proteins [SAPs]) recombine complementary dsDNA sequences that contain 3'-ssDNA overhangs. Several paralogues exist - this version is RecT-like but other versions in staphylococcal phages can include Sak4, Sak, Erf and DUF2800. Prophage SAPs are typically paired with a single-strand DNA exonuclease and a single-strand DNA-binding protein.
⊟Orthologs[edit | edit source]
COL:
—
N315:
—
NCTC8325:
—
04-02981:
—
08BA02176:
—
11819-97:
—
6850:
—
71193:
—
ECT-R 2:
—
ED133:
—
ED98:
—
HO 5096 0412:
—
JH1:
SaurJH1_0329
JH9:
SaurJH9_0320
JKD6008:
SAA6008_02040 (recT)
JKD6159:
—
JSNZ:
—
LGA251:
—
M013:
—
MRSA252:
—
MSHR1132:
—
MSSA476:
—
Mu3:
—
Mu50:
—
MW2:
—
RF122:
—
ST398:
—
T0131:
SAT0131_02121
TCH60:
—
TW20:
SATW20_03400
USA300_TCH1516:
USA300HOU_1991 (recT)
VC40:
—
⊟Genome Viewer[edit | edit source]
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
Newman MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYSPSNAMKQAWLQISQDN
USA300_FPR3757 MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYSPSNAMKQAWLQISQDN
************************************************************
Newman KLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQRSYHGNVMMLKRDAGAQDV
USA300_FPR3757 KLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQRSYHGNVMMLKRDAGAQDV
************************************************************
Newman VAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIGAYCTIVFNDGRDNYIEVMTI
USA300_FPR3757 VAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIGAYCTIVFNDGRDNYIEVMTI
************************************************************
Newman EQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVINRAAKRYINTSTDSNIFKYAQE
USA300_FPR3757 EQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVINRAAKRYINTSTDSNLFKYAQE
*****************************************************:******
Newman SEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYTELENDKPIDVSDFEEIKEPATEKE
USA300_FPR3757 SEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYTELENDKPIDVSDFEEIKEPATEKE
************************************************************
Newman SEEEPF
USA300_FPR3757 SEEEPF
******
- ↑ J P Muyrers, Y Zhang, F Buchholz, A F Stewart
RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners.
Genes Dev: 2000, 14(15);1971-82
[PubMed:10921910] [WorldCat.org] (P p)Lakshminarayan M Iyer, Eugene V Koonin, L Aravind
Classification and evolutionary history of the single-strand annealing proteins, RecT, Redbeta, ERF and RAD52.
BMC Genomics: 2002, 3;8
[PubMed:11914131] [WorldCat.org] [DOI] (P p)Simanti Datta, Nina Costantino, Xiaomei Zhou, Donald L Court
Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages.
Proc Natl Acad Sci U S A: 2008, 105(5);1626-31
[PubMed:18230724] [WorldCat.org] [DOI] (I p)Maan M Neamah, Ignacio Mir-Sanchis, María López-Sanz, Sonia Acosta, Ignacio Baquedano, Andreas F Haag, Alberto Marina, Silvia Ayora, José R Penadés
Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus.
Nucleic Acids Res: 2017, 45(11);6507-6519
[PubMed:28475766] [WorldCat.org] [DOI] (I p)Janine Bowring, Maan M Neamah, Jorge Donderis, Ignacio Mir-Sanchis, Christian Alite, J Rafael Ciges-Tomas, Elisa Maiques, Iltyar Medmedov, Alberto Marina, José R Penadés
Pirating conserved phage mechanisms promotes promiscuous staphylococcal pathogenicity island transfer.
Elife: 2017, 6;
[PubMed:28826473] [WorldCat.org] [DOI] (I e)Kamil Steczkiewicz, Eric Prestel, Elena Bidnenko, Agnieszka K Szczepankowska
Expanding Diversity of Firmicutes Single-Strand Annealing Proteins: A Putative Role of Bacteriophage-Host Arms Race.
Front Microbiol: 2021, 12;644622
[PubMed:33959107] [WorldCat.org] [DOI] (P e)Chrispin Chaguza, Joshua T Smith, Spencer A Bruce, Robert Gibson, Isabella W Martin, Cheryl P Andam
Prophage-encoded immune evasion factors are critical for Staphylococcus aureus host infection, switching, and adaptation.
Cell Genom: 2022, 2(11);
[PubMed:36465278] [WorldCat.org] [DOI] (I p)