PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1960 [new locus tag: SAUSA300_RS10750 ]
• pan locus tag^?: SAUPAN001397000
• symbol: SAUSA300_1960
• pan gene symbol^?: recT
• synonym:
• product: putative phage-related DNA recombination protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1960 [new locus tag: SAUSA300_RS10750 ]
• symbol: SAUSA300_1960
• product: putative phage-related DNA recombination protein
• replicon: chromosome
• strand: -
• coordinates: 2116730..2117650
• length: 921
• essential: unknown

⊟Accession numbers

• Gene ID: 3913275 NCBI
• RefSeq: YP_494611 NCBI
• BioCyc: see SAUSA300_RS10750
• MicrobesOnline: 1293475 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
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  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGACTGAAAATAATAAATTACAAACTATTGAACAACAATTAGTACAAGAAAAGAACGTA
  TCTGACAACGTATTAAACAAAGTGAGAGTTTTAGAGTCACAAGGCAATTTGGAATTGCCA
  AATGATTATTCACCAAGTAATGCCATGAAACAAGCATGGTTACAAATCAGCCAAGATAAC
  AAATTAATGAGTTGTAACGATACAAGCAAAGCAAATGCCTTATTAGACATGGTAACGCAA
  GGTTTAAATCCAGCTAAAAATCAATGCTACTTTATTCCTTACGGCAACAAAATGCAGTTA
  CAACGTAGCTATCACGGTAATGTAATGATGTTAAAACGTGATGCAGGTGCTCAAGATGTT
  GTTGCTCAAGTGATTTATAAAGGCGATACATTCAAGCAAGAAATGGGAGAAACAGGACGT
  ATCAAAGCGATTAAACACGAACAAGACTTCTTTAACATCGACAAAGAAAACATTATCGGT
  GCGTACTGCACAATCGTATTTAATGATGGACGAGATAACTATATTGAAGTCATGACTATT
  GAACAAATTAAACAAGCATGGATGCAGTCATCAATGATTAAAGATGAAAAAGCATTACAA
  AATTCTAAAACACATAATAATTTCAAAGAAGAAATGGCTAAAAAAACAGTTATCAATAGA
  GCTGCTAAACGTTATATCAACACATCAACAGATAGCAATCTTTTCAAATACGCACAAGAA
  TCCGAACAACGTCAACGCAAAGAAGTGTTGGACGCAGAAGTTGAAGAAAATGCAAATCAA
  GAACAATTGGACTTTGAACAACCAGTTCTTGAAGAAGCACAATACACAGAATTAGAAAAT
  GATAAGCCTATTGATGTATCTGACTTTGAAGAAATAAAAGAACCTGCAACAGAAAAAGAA
  AGCGAAGAAGAGCCATTTTAA
  60
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  921
  

⊟Protein

⊟General

• locus tag: SAUSA300_1960 [new locus tag: SAUSA300_RS10750 ]
• symbol: SAUSA300_1960
• description: putative phage-related DNA recombination protein
• length: 306
• theoretical pI: 4.48199
• theoretical MW: 35336.2
• GRAVY: -0.878105

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair recombinase, phage RecT family (TIGR00616; HMM-score: 58.4)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions recombinase, phage RecT family (TIGR00616; HMM-score: 58.4)
• TheSEED  :
  • Phage recombination protein Bet
  DNA Metabolism DNA repair DNA repair, bacterial  Recombinational DNA repair protein RecT (prophage associated)
• PFAM:
  no clan defined RecT; RecT family (PF03837; HMM-score: 176.7)
  and 1 more
  Ribonuc_red_2_N; Class II vitamin B12-dependent ribonucleotide reductase (PF08471; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.046046
  • TAT(Tat/SPI): 0.000691
  • LIPO(Sec/SPII): 0.001129
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160614 NCBI
• RefSeq: YP_494611 NCBI
• UniProt: A0A0H2XGX3 UniProt

⊟Protein sequence

• MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYSPSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQRSYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIGAYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVINRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYTELENDKPIDVSDFEEIKEPATEKESEEEPF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1960 < SAUSA300_1961

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications