⊟Summary[edit | edit source]
- pan ID?: SAUPAN003394000
- symbol?: ecb
- synonym:
- description?: fibrinogen-binding protein
- fibrinogen-binding protein
- extracellular fibrinogen/complement-binding protein
- fibrinogen binding-like protein
- fibrinogen-binding protein-like protein
- exported protein
- extracellular fibrinogen binding protein
- Fibrinogen-binding protein-related
- fibrinogen binding-related protein
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3755250..3755592
- synteny block?: BlockID0024770
- occurrence?: in 100% of 34 strains
ecb (ehp) : staphylococcal C3b-binding immunomodulatory protein Ecb [1]
• A crystal structure is available : 2NOJ
The second staphylococcal core genome immune evasion cluster (IEC-2) encodes the extracellular complement C3b-binding immunomodulatory protein Ecb. This small protein prevents complement C5 activation and adaptive immunity by forming higher order oligomers with cell surface-associated C3b and either itself or Efb. Unlike SCIN proteins, Ecb does not act on C3 convertase but rather binds to C3b to prevent further activation of C5 pathways. Because it only binds to C3b, Ecb (and by extension Efb) only prevents C3 deposition onto the staphylococcal cell by the alternative activation pathway. Therefore C3d-opsonized staphylococci still occur by the classical or lectin pathways. However, membrane-bound C3d complement maturation into activated C5 convertase is blocked by Efb and Ecb binding as well as host B-cell and T-cell adaptive immunity via blocking of immune cell CR2 receptor/staphylococcal-opsonized-C3d coactivation . Please note that Ecb is effectively a homologue of the C3b-binding C-terminal domain from Efb. All Efb fibrinogen-binding activity is located in its N-terminal domain; however, due to homology to the Efb C-terminal domain, Ecb is often misannotated as a "fibrinogen-binding protein". It contains a signal peptidase secretion signal and is cleaved between alanine-29 and glutamine-30 to generate mature Ecb.
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQTNFKKQVNKKVVDAQK
N315 MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQTNFKKQVNKKVVDAQK
NCTC8325 MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQTNFKKQVNKKVVDAQK
Newman MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQTNFKKQVNKKVVDAQK
USA300_FPR3757 MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQTNFKKQVNKKVVDAQK
************************************************************
COL AVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYNTLK
N315 AVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYNTLK
NCTC8325 AVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYNTLK
Newman AVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYNTLK
USA300_FPR3757 AVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYNTLK
***:*********************************************
- ↑ Ilse Jongerius, Jörg Köhl, Manoj K Pandey, Maartje Ruyken, Kok P M van Kessel, Jos A G van Strijp, Suzan H M Rooijakkers
Staphylococcal complement evasion by various convertase-blocking molecules.
J Exp Med: 2007, 204(10);2461-71
[PubMed:17893203] [WorldCat.org] [DOI] (P p)Brandon L Garcia, Kasra X Ramyar, Daniel Ricklin, John D Lambris, Brian V Geisbrecht
Advances in understanding the structure, function, and mechanism of the SCIN and Efb families of Staphylococcal immune evasion proteins.
Adv Exp Med Biol: 2012, 946;113-33
[PubMed:21948365] [WorldCat.org] [DOI] (P p)