⊟Summary[edit | edit source]
- pan ID?: SAUPAN004447000
- symbol?: —
- synonym:
- description?: rRNA methyltransferase
- rRNA methyltransferase
- class I SAM-dependent methyltransferase
- methyltransferase domain-containing protein
- SAM-dependent methyltransferase, putative
- SAM-dependent methyltransferase, MraW methylase family
- conserved protein YtqB
- putative rRNA methylase
- rRNA methylase family protein
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 4694900..4695463
- synteny block?: BlockID0034440
- occurrence?: in 100% of 34 strains
mnmM : tRNA 5-aminomethyl-2-thiouridine(34) methyltransferase [1]
• A crystal structure is available : 8H1B
Transfer RNAs containing uridine at wobble anticodon position 34 often require modifications to provide maximal fidelity during protein synthesis starting from a shared 5-carboxymethylaminomethyl-2-thiouridine(34) (“cmnm5s2U”, “$”) generated by MnmAEG. One such modification involves SAM-dependent oxidative decarboxylation of 5-carboxymethylaminomethyl-2-thiouridine(34) to generate 5-aminomethyl-2-thiouridine(34) and subsequent SAM-dependent methylation to 5-methylaminomethyl-2-thiouridine(34) by the MnmLM complex. A small amount of 5-aminomethyl-2-thiouridine(34) can also be provided by MnmEG using ammonium as a substrate. These two enzymatic activities are shared in the bifunctional enzyme MnmC in gram-negative bacteria.
tRNA : nm5s2U (34) → mnm5s2U (34)
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
COL | |
N315 | |
NCTC8325 | |
Newman | |
USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPEGHVYGFDIQDLALENT
N315 MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPEGHVYGFDIQDLALENT
NCTC8325 MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPEGHVYGFDIQDLALENT
Newman MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPEGHVYGFDIQDLALENT
USA300_FPR3757 MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPEGHVYGFDIQDLALENT
************************************************************
COL RDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYLPKGDKSIVTKPDTTIQAI
N315 RDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYLPKGDKSIVTKPDTTIQAI
NCTC8325 RDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYLPKGDKSIVTKPDTTIQAI
Newman RDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYLPKGDKSIVTKPDTTIQAI
USA300_FPR3757 RDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYLPKGDKSIVTKPDTTIQAI
************************************************************
COL NSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQKHAQVLQYQFLNQRNHAPFI
N315 NSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQKHAQVLQYQFLNQRNHAPFI
NCTC8325 NSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQKHAQVLQYQFLNQRNHAPFI
Newman NSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQKHAQVLQYQFLNQRNHAPFI
USA300_FPR3757 NSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQKHAQVLQYQFLNQRNHAPFI
************************************************************
COL CAIEKIS
N315 CAIEKIS
NCTC8325 CAIEKIS
Newman CAIEKIS
USA300_FPR3757 CAIEKIS
*******
- ↑ Gyuhyeok Cho, Jangmin Lee, Jungwook Kim
Identification of a novel 5-aminomethyl-2-thiouridine methyltransferase in tRNA modification.
Nucleic Acids Res: 2023, 51(4);1971-1983
[PubMed:36762482] [WorldCat.org] [DOI] (I p)