PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0055 [new locus tag: SAUSA300_RS00280 ]
• pan locus tag^?: SAUPAN000809000
• symbol: SAUSA300_0055
• pan gene symbol^?: adhC
• synonym:
• product: alcohol dehydrogenase, zinc-containing

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0055 [new locus tag: SAUSA300_RS00280 ]
• symbol: SAUSA300_0055
• product: alcohol dehydrogenase, zinc-containing
• replicon: chromosome
• strand: +
• coordinates: 64125..65222
• length: 1098
• essential: unknown

⊟Accession numbers

• Gene ID: 3914409 NCBI
• RefSeq: YP_492774 NCBI
• BioCyc: GH3C-55 BioCyc
• MicrobesOnline: 1291570 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  
  ATGGGGGCTAAAAAGCCCTATAAAGAATCTAAACCTTTAAAAATTGAATACTTAGAACTT
  GATAATCCTAGCGAACACGAAGTATTAATTAAAATTCATGCAGCTGGATTATGTCACTCT
  GATTTGTCAGTAATTAATGGTAACAGACCTAGACCTTTACCTATGGCACTTGGTCATGAA
  GCTTCCGGTGAAGTAATTAAAGTTGGAAAAGCTGTTACAAGAGTTAGCGAAGGAGATCAT
  GTGGTATGCACATTTATTCCCAGTTGTGGAAAATGTATCCCATGTAAAGAAGGACGTCCT
  GCATTATGTGAAAACGGAGCAATATCTAATGAAAAAGGCGAAATGTTAGAGGGAGGGATG
  CGTTTATCTAATGATGAAGGAAAAGTATATCATCACTTGGGGATATCAGGTTTTGCTGAA
  TATTCTGTTGTCTCTGAAAACTCTATAGTTAAAATTGATAAAAAAATACCTTTTGAACGT
  GCAGCTGCATTTGGTTGTGCTATTATCACAGGTATCGGTGCTGTGGTGAATACAGCCCAA
  ATTCGTTCTGGTAGTAATGTAGCGGTTGTTGGTTTAGGAGGTATTGGATTGAATGCTATT
  ATTGGAGCTAAACTAGCGGGAGCCAATGAAATTATTGCTTTAGATATTAACGAAGATAAA
  TTTGAATTAGCAAAGCAATTTGGGGCTACAGCAACATTTAATTCAAGCGATAAAGATATC
  GATGAGCAAATTAAAGAATATATTCCTGGCGGAGTAGAATATGCTTTTGAAACAGCGGGT
  GTAGTGCCAGCTATGAAAGTTGCTTATCAGATTACTAAACGAGGGGGGACAACTGTAACA
  ACAGGACTGCCTAATCCTAAAGATAATTTTTCTTTCCCTCAAGTTACTTTAGCGGCTGAA
  GAACGTACCATTAAAGGATCATATGTAGGAAGTTGTGTACCTGACAGAGATATACCAAGG
  TTCGTTAATCTATACAACCAAGGACGTTTAAATATTGATTCACTCATCAGTGAGGTTATC
  ACTTTAGATGAAATTAATGAGGGATTTGATCGTTTGTCTAATGGTGAAGTAGGTAGAATT
  ATAATGAAGATGCATTGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1098
  

⊟Protein

⊟General

• locus tag: SAUSA300_0055 [new locus tag: SAUSA300_RS00280 ]
• symbol: SAUSA300_0055
• description: alcohol dehydrogenase, zinc-containing
• length: 365
• theoretical pI: 5.90054
• theoretical MW: 39116.5
• GRAVY: -0.106301

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 329.9)
  Cellular processes Cellular processes Detoxification S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase (TIGR02818; EC 1.1.1.1,1.1.1.284; HMM-score: 306.2)
  Metabolism Energy metabolism Fermentation S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase (TIGR02818; EC 1.1.1.1,1.1.1.284; HMM-score: 306.2)
  Cellular processes Cellular processes Detoxification S-(hydroxymethyl)mycothiol dehydrogenase (TIGR03451; EC 1.1.1.306; HMM-score: 285.6)
  and 10 more
  Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 142.7)
  putative phosphonate catabolism associated alcohol dehydrogenase (TIGR03366; HMM-score: 134.4)
  6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 123.8)
  Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 89.4)
  Metabolism Central intermediary metabolism One-carbon metabolism formaldehyde dehydrogenase, glutathione-independent (TIGR02819; EC 1.2.1.46; HMM-score: 86.8)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02822; EC 1.-.-.-; HMM-score: 61)
  crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 58)
  Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 35.3)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 28.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 23.8)
• TheSEED  :
  • Alcohol dehydrogenase (EC 1.1.1.1)
  Carbohydrates Fermentation Fermentations: Lactate  Alcohol dehydrogenase (EC 1.1.1.1)
  and 2 more
  Carbohydrates Fermentation Fermentations: Mixed acid  Alcohol dehydrogenase (EC 1.1.1.1)
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Alcohol dehydrogenase (EC 1.1.1.1)
• PFAM:
  NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 102.8)
  GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 101)
  and 5 more
  NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 21.1)
  Glu_dehyd_C; Glucose dehydrogenase C-terminus (PF16912; HMM-score: 19.5)
  ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 18.3)
  no clan defined NADH_4Fe-4S; NADH-ubiquinone oxidoreductase-F iron-sulfur binding region (PF10589; HMM-score: 15.3)
  NADP_Rossmann (CL0063) UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 2
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016638
  • TAT(Tat/SPI): 0.004386
  • LIPO(Sec/SPII): 0.000745
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161898 NCBI
• RefSeq: YP_492774 NCBI
• UniProt: A0A0H2XI08 UniProt

⊟Protein sequence

• MGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHAAGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKCIPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIVKIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGANEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMKVAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNLYNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications