PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00280 [old locus tag: SAUSA300_0055 ]
• pan locus tag^?: SAUPAN000809000
• symbol: SAUSA300_RS00280
• pan gene symbol^?: adhC
• synonym:
• product: alcohol dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00280 [old locus tag: SAUSA300_0055 ]
• symbol: SAUSA300_RS00280
• product: alcohol dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 64095..65222
• length: 1128
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (64095..65222) NCBI
• BioCyc: SAUSA300_RS00280 BioCyc
• MicrobesOnline: see SAUSA300_0055

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
  541
  601
  661
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  841
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  1021
  1081
  
  GTGAAAACTAGAGCAGCAGTTTTACATGAAATGGGGGCTAAAAAGCCCTATAAAGAATCT
  AAACCTTTAAAAATTGAATACTTAGAACTTGATAATCCTAGCGAACACGAAGTATTAATT
  AAAATTCATGCAGCTGGATTATGTCACTCTGATTTGTCAGTAATTAATGGTAACAGACCT
  AGACCTTTACCTATGGCACTTGGTCATGAAGCTTCCGGTGAAGTAATTAAAGTTGGAAAA
  GCTGTTACAAGAGTTAGCGAAGGAGATCATGTGGTATGCACATTTATTCCCAGTTGTGGA
  AAATGTATCCCATGTAAAGAAGGACGTCCTGCATTATGTGAAAACGGAGCAATATCTAAT
  GAAAAAGGCGAAATGTTAGAGGGAGGGATGCGTTTATCTAATGATGAAGGAAAAGTATAT
  CATCACTTGGGGATATCAGGTTTTGCTGAATATTCTGTTGTCTCTGAAAACTCTATAGTT
  AAAATTGATAAAAAAATACCTTTTGAACGTGCAGCTGCATTTGGTTGTGCTATTATCACA
  GGTATCGGTGCTGTGGTGAATACAGCCCAAATTCGTTCTGGTAGTAATGTAGCGGTTGTT
  GGTTTAGGAGGTATTGGATTGAATGCTATTATTGGAGCTAAACTAGCGGGAGCCAATGAA
  ATTATTGCTTTAGATATTAACGAAGATAAATTTGAATTAGCAAAGCAATTTGGGGCTACA
  GCAACATTTAATTCAAGCGATAAAGATATCGATGAGCAAATTAAAGAATATATTCCTGGC
  GGAGTAGAATATGCTTTTGAAACAGCGGGTGTAGTGCCAGCTATGAAAGTTGCTTATCAG
  ATTACTAAACGAGGGGGGACAACTGTAACAACAGGACTGCCTAATCCTAAAGATAATTTT
  TCTTTCCCTCAAGTTACTTTAGCGGCTGAAGAACGTACCATTAAAGGATCATATGTAGGA
  AGTTGTGTACCTGACAGAGATATACCAAGGTTCGTTAATCTATACAACCAAGGACGTTTA
  AATATTGATTCACTCATCAGTGAGGTTATCACTTTAGATGAAATTAATGAGGGATTTGAT
  CGTTTGTCTAATGGTGAAGTAGGTAGAATTATAATGAAGATGCATTGA
  60
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  1020
  1080
  1128
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS00280 [old locus tag: SAUSA300_0055 ]
• symbol: SAUSA300_RS00280
• description: alcohol dehydrogenase
• length: 375
• theoretical pI: 6.25011
• theoretical MW: 40253.9
• GRAVY: -0.1096

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 332.2)
  Cellular processes Cellular processes Detoxification S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase (TIGR02818; EC 1.1.1.1,1.1.1.284; HMM-score: 306.3)
  Metabolism Energy metabolism Fermentation S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase (TIGR02818; EC 1.1.1.1,1.1.1.284; HMM-score: 306.3)
  Cellular processes Cellular processes Detoxification S-(hydroxymethyl)mycothiol dehydrogenase (TIGR03451; EC 1.1.1.306; HMM-score: 285.5)
  and 10 more
  Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 142.5)
  putative phosphonate catabolism associated alcohol dehydrogenase (TIGR03366; HMM-score: 134.1)
  6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 123.7)
  Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 89.2)
  Metabolism Central intermediary metabolism One-carbon metabolism formaldehyde dehydrogenase, glutathione-independent (TIGR02819; EC 1.2.1.46; HMM-score: 87.5)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02822; EC 1.-.-.-; HMM-score: 60.8)
  crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 58.4)
  Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 35.3)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 28.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 23.7)
• TheSEED: see SAUSA300_0055
• PFAM:
  NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 102.9)
  GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 100.9)
  and 5 more
  NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 21)
  Glu_dehyd_C; Glucose dehydrogenase C-terminus (PF16912; HMM-score: 19.5)
  ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 18.2)
  no clan defined NADH_4Fe-4S; NADH-ubiquinone oxidoreductase-F iron-sulfur binding region (PF10589; HMM-score: 15.3)
  NADP_Rossmann (CL0063) UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 2
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012192
  • TAT(Tat/SPI): 0.000761
  • LIPO(Sec/SPII): 0.000427
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446779903 NCBI
• RefSeq: WP_000857159 NCBI
• UniProt: see SAUSA300_0055

⊟Protein sequence

• MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHAAGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKCIPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIVKIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGANEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMKVAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNLYNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications