PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0066 [new locus tag: SAUSA300_RS00335 ]
• pan locus tag^?: SAUPAN000815000
• symbol: argR
• pan gene symbol^?: argR
• synonym:
• product: arginine repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0066 [new locus tag: SAUSA300_RS00335 ]
• symbol: argR
• product: arginine repressor
• replicon: chromosome
• strand: -
• coordinates: 74617..75063
• length: 447
• essential: unknown

⊟Accession numbers

• Gene ID: 3915260 NCBI
• RefSeq: YP_492785 NCBI
• BioCyc: see SAUSA300_RS00335
• MicrobesOnline: 1291581 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAAAGAAAAACGTTTAAATCTTATCTTAACTGTTATACAACAGAATCAGTTTAAT
  AAAAAACAACAAATAGTGGATTACATGGCAAGACATTTTGGAGTTTACTATAGCTTGACA
  ACTATTTCTCGTGACTTACAAGAATTAGAAATTTACAAAATCCCTGTTGAAAATAAAAAG
  TATATTTACAAGAAAATAAATCAAACAAATCAATTAAGTGCAAAAAAACAATTAGAAATA
  TTTAGTGATGAGATTATTGAATTTATAACGCTAAATAACTATGTCTTAATAAAAACATCT
  CCTGGCTTTGCTCAAAGTATAAGTTATTACATAGATCAATTACAAATGAAAGAAATATTA
  GGAATTATTGGAGGTAACGATACTTTGATGATTTTGACTTCTTCAAATGAAATAGCAGAA
  TTTGTTTGTTATCAATTATTCCCTTAA
  60
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  447
  

⊟Protein

⊟General

• locus tag: SAUSA300_0066 [new locus tag: SAUSA300_RS00335 ]
• symbol: ArgR
• description: arginine repressor
• length: 148
• theoretical pI: 8.84407
• theoretical MW: 17390.1
• GRAVY: -0.187838

⊟Function

• TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family arginine repressor (TIGR01529; HMM-score: 83.5)
  Signal transduction Regulatory functions DNA interactions arginine repressor (TIGR01529; HMM-score: 83.5)
• TheSEED  :
  • Arginine pathway regulatory protein ArgR, repressor of arg regulon
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Arginine pathway regulatory protein ArgR, repressor of arg regulon
  and 2 more
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Arginine pathway regulatory protein ArgR, repressor of arg regulon
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Arginine pathway regulatory protein ArgR, repressor of arg regulon
• PFAM:
  no clan defined Arg_repressor_C; Arginine repressor, C-terminal domain (PF02863; HMM-score: 60.3)
  and 5 more
  HTH (CL0123) Arg_repressor; Arginine repressor, DNA binding domain (PF01316; HMM-score: 40.3)
  HTH_33; Winged helix-turn helix (PF13592; HMM-score: 14)
  no clan defined VPS9; Vacuolar sorting protein 9 (VPS9) domain (PF02204; HMM-score: 13.5)
  HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 12.9)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004418
  • TAT(Tat/SPI): 0.000251
  • LIPO(Sec/SPII): 0.000682
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162413 NCBI
• RefSeq: YP_492785 NCBI
• UniProt: A0A0H2XKJ2 UniProt

⊟Protein sequence

• MKKEKRLNLILTVIQQNQFNKKQQIVDYMARHFGVYYSLTTISRDLQELEIYKIPVENKKYIYKKINQTNQLSAKKQLEIFSDEIIEFITLNNYVLIKTSPGFAQSISYYIDQLQMKEILGIIGGNDTLMILTSSNEIAEFVCYQLFP

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications