PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0071 [new locus tag: SAUSA300_RS00360 ]
• pan locus tag^?: SAUPAN000819000
• symbol: SAUSA300_0071
• pan gene symbol^?: —
• synonym:
• product: ISSep1-like transposase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0071 [new locus tag: SAUSA300_RS00360 ]
• symbol: SAUSA300_0071
• product: ISSep1-like transposase
• replicon: chromosome
• strand: +
• coordinates: 78432..79445
• length: 1014
• essential: unknown

⊟Accession numbers

• Gene ID: 3914036 NCBI
• RefSeq: YP_492789 NCBI
• BioCyc: see SAUSA300_RS00360
• MicrobesOnline: 1291586 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGAACGATTTTGTTGTGTAAATCAAATTAACTATATTCAAATGAATCCGTTAGAAGCC
  AAATTTAAAACGAGCGCTCTAAGATCATGGAAAACTGATCAGGCAGATGCTCATAAGCTT
  GCTTGTTTAGGACCGACGCTCAAACAAACAGGCAGCTTACCTATACATGAGTTAATATTC
  TTTGAATTAAGAGAACGTGCCCGTTTTCATCTAGAAATCGAGAATGAACAAAATCGACTT
  AAATTTCAGATTCTTGAATTACTCCATCAAACATTCCCTGGTTTAGAAAGATTATTTAGT
  AGTCGATATTCAATCATTGCACTCAACATCGCAGAAATTTTTACTCATCCAGACGTGGTT
  CTTGATATCGACAAGGATGTACTTATTACACATATATTCAATTCTACAGATAAGGGAATG
  TCAATGGATAAAGCTACAAAATATGCACTTCAATTAAGAGTGATTGCTCAAGAAAGCTAT
  CCTAATGTCGATAGACATTCCTTTCTAGTCGAAAAATTACGCTTACTTATTCAACAATTA
  AAACAATCTATTCATCATCTCAAACAATTAGATGATGCCATGATTCAATTAGCACAACAA
  CTCGATTATTTTGAAAATATTCATTCGATACCTGGTATTGGTAAGCTAAGCACAGCTATG
  ATTATTGGGGAGATTGGTGATATTAAGCGATTTAAATCAAATAAACAACTCAACGCTTTT
  GTAGGCATTGATATCAAACGATATCAATCAGGTCATACACACTGTAGAGATACCATCAAC
  AAGCGTGGTAATAAAAAAGCGAGAAAACTTTTATTTTGGGTGATTATGAATATAATAAGA
  GGGCAGCATCATTATGACAATCATGTCGTCGATTATTACTACAAACTAAGAAAGCAGCCT
  AATGAGAAACCTCATAAGACTGCCATCATTGCTTGTATAAATCGATTATTAAAAACGATT
  CATTATCTGGTAATGAATCATAAATTGTACGATTATCAAATGTCACCACATTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_0071 [new locus tag: SAUSA300_RS00360 ]
• symbol: SAUSA300_0071
• description: ISSep1-like transposase
• length: 337
• theoretical pI: 9.82964
• theoretical MW: 39540.7
• GRAVY: -0.359941

⊟Function

• TIGRFAM:
• TheSEED  :
  • Mobile element protein
• PFAM:
  HHH (CL0198) Transposase_20; Transposase IS116/IS110/IS902 family (PF02371; HMM-score: 76.3)
  and 4 more
  no clan defined DEDD_Tnp_IS110; Transposase (PF01548; HMM-score: 33.8)
  Hepatitis_core; Hepatitis core antigen (PF00906; HMM-score: 15.6)
  DUF3677; Protein of unknown function (DUF3677) (PF12432; HMM-score: 15.3)
  DUF5340; Family of unknown function (DUF5340) (PF17275; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005166
  • TAT(Tat/SPI): 0.000421
  • LIPO(Sec/SPII): 0.001056
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161793 NCBI
• RefSeq: YP_492789 NCBI
• UniProt: A0A0H2XHT9 UniProt

⊟Protein sequence

• MERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTLKQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSIIALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNVDRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMIIGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNIIRGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications