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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0115 [new locus tag: SAUSA300_RS00595 ]
  • pan locus tag?: SAUPAN000911000
  • symbol: sirC
  • pan gene symbol?: sirC
  • synonym:
  • product: iron compound ABC transporter permease SirC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0115 [new locus tag: SAUSA300_RS00595 ]
  • symbol: sirC
  • product: iron compound ABC transporter permease SirC
  • replicon: chromosome
  • strand: -
  • coordinates: 131235..132233
  • length: 999
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACCGAAAAGATTAATAAAAAAGACAATTACCATCTCATCTTCGCGTTAATCTTTTTA
    GCCATCGTTTCAGTGGTAAGTATGATGATTGGTTCAAGCTTTATACCATTACAACGCGTA
    CTGATGTACTTTATAAATCCAAATGACAGTATGGATCAATTCACTTTAGAAGTATTACGC
    TTACCTCGCATTACACTTGCGATTTTAGCAGGTGCCGCACTAGGAATGAGTGGTTTAATG
    TTGCAAAATGTATTAAAAAATCCAATTGCCTCACCTGATATTATCGGTATCACAGGTGGT
    GCTAGCTTAAGTGCTGTTGTCTTTATTGCATTTTTCAGCCATTTAACAATACATTTACTT
    CCACTATTTGCAGTATTAGGTGGCGCAGTTGCAATGATGATACTATTAGTGTTTCAAACG
    AAAGGACAAATACGCCCGACAACACTCATAATCATCGGTATTTCGATGCAAACGTTGTTT
    ATTGCGCTTGTCCAAGGATTACTCATTACAACGAAGCAATTATCTGCTGCCAAAGCTTAT
    ACATGGCTAGTCGGAAGTCTTTACGGTGCTACGTTTAAAGATACAATCATTTTGGGTATG
    GTTATTTTAGCTGTTGTGCCGTTGTTATTTCTTGTTATACCAAAAATGAAAATATCTATA
    CTTGATGACCCTGTAGCGATTGGCTTAGGCTTACATGTACAACGTATGAAACTAATCCAA
    TTAATCACTTCTACTATACTCGTATCTATGGCAATCAGTTTAGTAGGTAACATTGGGTTT
    GTCGGTTTAATCGCACCACATATCGCGAAAACAATCGTTCGCGGAAGTTATGCTAAAAAG
    TTACTAATGTCAGCAATGATTGGTGCCATATCAATTGTTATTGCAGACTTAATTGGGCGT
    ACCTTATTCTTGCCTAAAGAAGTGCCAGCAGGTGTATTTATTGCTGCTTTTGGTGCCCCA
    TTCTTCATATACTTATTATTAACCGTGAAAAAGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    999

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0115 [new locus tag: SAUSA300_RS00595 ]
  • symbol: SirC
  • description: iron compound ABC transporter permease SirC
  • length: 332
  • theoretical pI: 10.6652
  • theoretical MW: 35729.5
  • GRAVY: 1.11657

⊟Function[edit | edit source]

  • TIGRFAM:
    proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 149.6)
    and 1 more
    Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, permease subunit (TIGR03770; HMM-score: 27.5)
  • TheSEED  :
    • Siderophore staphylobactin ABC transporter, permease protein SirC
    Iron acquisition and metabolism Siderophores Siderophore Staphylobactin  Siderophore staphylobactin ABC transporter, permease protein SirC
  • PFAM:
    Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 278.5)
    and 2 more
    ABC-3; ABC 3 transport family (PF00950; HMM-score: 18.3)
    ISOCOT_Fold (CL0246) Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.029257
    • TAT(Tat/SPI): 0.000776
    • LIPO(Sec/SPII): 0.016007
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYFINPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGASLSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLFIALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKISILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGSYAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)

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