From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0225 [new locus tag: SAUSA300_RS01195 ]
  • pan locus tag?: SAUPAN001092000
  • symbol: SAUSA300_0225
  • pan gene symbol?: fadA
  • synonym:
  • product: putative acyl-CoA acetyltransferase FadA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0225 [new locus tag: SAUSA300_RS01195 ]
  • symbol: SAUSA300_0225
  • product: putative acyl-CoA acetyltransferase FadA
  • replicon: chromosome
  • strand: -
  • coordinates: 268835..270019
  • length: 1185
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAAGAAGCATACATTGTAGCTTATGGGCGTTCAGCCGCAGCGAAAGCAAAGCAAGGC
    GCATTATTCCACGAAAGACCTGATGATGTCGCAGCCAAAGTATTACAAGGCGTATTGAAA
    CGTATTGACGGAAAATTCAATAAGAATATGATTGAAGATGTCATTGTTGGTACGGCTTTT
    CCAGAAGGATTACAAGGCCAAAACATTGCACGAACGATTGCATTGCGTGCGGGATTATCT
    GACACGGTACCGGGTCAAACAGTGAATCGCTACTGCTCATCAGGATTACAAACCATCGCG
    ATTGCAGCCAATCAAATTATGGCTGGTCAAGGAGATATACTTGTAGCTGGTGGCGTTGAA
    TTGATGAGTGCCGTACCAATGGGTGGCAACGAGCCCACAAACAATCCAACCTTACAATAT
    GATGATATAGGTGCGTCATATCCTATGGGTTTAACTGCTGAAAATGTAGCATCCCAATTT
    GACGTATCACGTGAAGATCAAGATGCTTATGCTGTCAGAAGTCATCAACGTGCCTATGAC
    GCACAACGTGATGGTCGGTTCAAAGATGAAATTATTCCAATACAAGTAAACTCAGTTGAA
    TATACAAACGCAGGACCAAAAGTACACACAAATATCTTTGACCAAGATGAATTTATACGC
    CCTGACACCACGATGGAGGCATTAGCCAAATTACGTACAGTATTTAAAGCTGACGGCACT
    GTGACTGCAGGAACATCTGCCCCACTTTCTGATGGTGCAGGATTTGTAGTTTTAATGTCT
    GGAGATAAAGTGAAAGAACTCGGCGTGACACCTATTGCACGATTCGTTGGTTTTAAGGCA
    GTAGGCGTTGACCCGAAAATTATGGGTATTGGGCCTGCATATGCGATTCCTGAAGTATTG
    TCACTCAGCAATCTATCTGTTGAAGACATTGATTTGATCGAATTGAACGAAGCATTTGCT
    TCTCAAACGATTGCATCTATTAAAGAAGTAGGTCTAGATATATCACGTACGAATGTGAAT
    GGTGGCGCTATTGCTTTAGGTCATCCATTAGGTGCTACAGGCGCAATGTTAACCGCGCGT
    TTACTTAATGAAATGGGTAGACGTCCCGATAGCCGTTACGGCATGGTTACGATGTGTATT
    GGTGTCGGCATGGGTGCAGCTGCTATATTTGAATATGTGCGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0225 [new locus tag: SAUSA300_RS01195 ]
  • symbol: SAUSA300_0225
  • description: putative acyl-CoA acetyltransferase FadA
  • length: 394
  • theoretical pI: 4.8083
  • theoretical MW: 41804.3
  • GRAVY: -0.0139594

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 454.8)
    and 4 more
    Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 348.8)
    3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 343.6)
    Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 198.1)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 32.5)
  • TheSEED  :
    • 3-ketoacyl-CoA thiolase (EC 2.3.1.16)
    Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
    and 3 more
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
    Fatty Acids, Lipids, and Isoprenoids Fatty Acids, Lipids, and Isoprenoids - no subcategory Polyhydroxybutyrate metabolism  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  • PFAM:
    Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 241.1)
    and 3 more
    Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 150.6)
    ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 22.6)
    P-loop_NTPase (CL0023) Podovirus_Gp16; Podovirus DNA encapsidation protein (Gp16) (PF05894; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007526
    • TAT(Tat/SPI): 0.000561
    • LIPO(Sec/SPII): 0.001163
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDGKFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIAANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQFDVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEFIRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFVGFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDISRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEYVR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SAUSA300_2203(rplD)50S ribosomal protein L4  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_0225&oldid=83917"