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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0226 [new locus tag: SAUSA300_RS01200 ]
- pan locus tag?: SAUPAN001093000
- symbol: SAUSA300_0226
- pan gene symbol?: fadB
- synonym:
- product: 3-hydroxyacyl-CoA dehydrogenase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0226 [new locus tag: SAUSA300_RS01200 ]
- symbol: SAUSA300_0226
- product: 3-hydroxyacyl-CoA dehydrogenase
- replicon: chromosome
- strand: -
- coordinates: 270049..272310
- length: 2262
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914735 NCBI
- RefSeq: YP_492940 NCBI
- BioCyc: see SAUSA300_RS01200
- MicrobesOnline: 1291741 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2221ATGACAATTAATAAAGTAACCGTTCTTGGCGCAGGCACAATGGGCGCTCAACTGGCAGCA
CTTTTTGTGAATGCTGGACTTAAAGTAAAACTATTAGATATTGTAGTGGACAAAAACGAT
CCAAATCTCATTGCGAAAAAATCTTACGATAAAATTACAGATAAGAAACGGCCGCTACTA
TTCGACTTAAATCTAGCGAGTCATTTAACATATGGTAATTTTGATGATGACTTGGTAAAT
GATGATGCTGATTTATATATCGAAGCAGTCAAAGAAGATATTGAAATTAAGCATGCTGTT
TGGCAACAAGTTCTACAACATGCTAAAGAAGATGCTTTATTCGCTACAAATACATCAGGT
ATTCCAATTAATGCGATTGCTCAAGCATTTAACGAGAAGGATCAAGAACGATTCTTTGGT
CTACATTTCTTTAACCCACCACGTATTATGAAATTAGTGGAGTTAATACCTACGTCACAC
ACGAAGGAATCTATTATATTAGATGTAAAAAATTTCGCGCAAAATGTGTTAGGTAAAGGT
GTCATTGTCGTCAATGATGTGCCTGGCTTTGTCGCAAATAGAGTCGGCACGCAAACAATG
AATGATATTATGTATCGCGCCGAGCAACACAAGATAAGCATTGTAGATGTGGATGCTTTA
ACTGGGCAAGCGATTGGTCGTCCTAAAACAGGTACATATGCGCTATCTGACCTAGTCGGT
TTAGATATTGCAGTGTCTGTAATTAAAGGCATGCAACAAGTACCTGAAGAAACACCTTAT
TTTCATGATGTCAAAATTGTAAATACGTTGTTTGACAATGGCGCACTCGGACGTAAAACG
AAACAAGGATTTTACAAAAAGGATAAAGAAACTAAAGCTCGACTTGTTTACGATGTTGAA
AAACAAGATTATGTACCTGTATCGCAACCACAATTACCAATTTTAAATGAATTTAATAAA
GACTTAGTGCATAACCTTGATACCATATTCAATGCGCAAGACGAAGCGGGACTATTTTTA
TGGGAGACATTACGTAATAATTTCTATTACTCTGCTATCAATGTACCTAAAGCTACCGAT
GATTTCCGAGACATAGACCGTGCGCTTGTCTGGGGGTTCAACTGGAAACTTGGTCCATTC
CAATTATGGGATGCAATGGGATACGAACGTGTTAAAACACGTATGGAAGACGAACTTGGA
GACTTACCACAATGGATTAGTGATTTAGATGGTGGCTTTTATAAACAAGATGAGACCATT
GAATATGCAACACCTATTTCTCACTTCGTAAAAGATGAACTTTGGGATAAAGGTGATGCC
AAACTTTCCGTAACTCATGATGATCAACTGTTACTGAAATTACAAAGTAAAAATAATGTC
ATTACCGATGAATTCAACGATGCGTTAGTTGATGCGATTGATTTACTGGAAAATGACCAT
TACACAAGTATGGTTATTTATGCAGATGGTAACAATTTCAGTGTGGGTGCTAACCTTTTC
TTAATGAAAAAGGCGCATGAAGACGGTCTTGTAGATGATGTCGTTGCACAATCAATTGAT
AAATTACATTATAGCTTTAATCGTTTGAAGTATAGTTTGAAACCAGTAGTCACAGCTGTT
CAAGGTCGTGCCTTAGGCGGTGGCTGTGAGCTTGTACTTTACTCACCTATTGTTGTCGCT
GCAAGTGAAACATATATCGGTCTTGTTGAAGCAGGTGTTGGCTTATTACCGAGTGGCGGT
GGCCTTGCAGAAATGGCTGATCGCATATTACGCACATCGCATAAGTTTGATGACAAACAA
ACTTCCATGACAAAAGTACTGACGAATATCGCATTTGCGAAAGTCTCTACAAATGCCTTT
GAGGCACGTCGTTATGGTTATTTACGTGATACAGATACGATTATTTTCAATACAGCACAA
CGTGTCGAAGTTGCGCTCAAACGTGCGAAATATGAAGCAGAAACAAACTATATTCCGAAT
CCTAGACATCAATATATCGCTTTAGGTGAAGACTTCAAAGCATTGATCCAAGGACAATTA
GATGCGCAAAGACGGGGTCATTTTATTAGCGACCATGATTATCATATTGCCTTAAATATC
GCCACAATTTTAGCGGGTGGTGATTTACCAAGAAATACATTTATCAATCAACGTTACATT
CAATCGTTGGAGAAAATTGGCTTTATTGACTTACTAAAATCTAAAAAATCATATGAAAGA
ATTGCACATATGTTAAAAACTGGTAAGCCATTACGTAATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0226 [new locus tag: SAUSA300_RS01200 ]
- symbol: SAUSA300_0226
- description: 3-hydroxyacyl-CoA dehydrogenase
- length: 753
- theoretical pI: 5.83805
- theoretical MW: 84637.8
- GRAVY: -0.246082
⊟Function[edit | edit source]
- ⊞reaction: EC 1.1.1.35 ExPASy
- ⊞TIGRFAM: fatty acid oxidation complex, alpha subunit, mitochondrial (TIGR02441; EC 1.1.1.35,4.2.1.17; HMM-score: 175)Fatty acid and phospholipid metabolism Degradation fatty oxidation complex, alpha subunit FadB (TIGR02437; EC 1.1.1.35,4.2.1.17,5.1.2.3,5.3.3.8; HMM-score: 168.1)Fatty acid and phospholipid metabolism Degradation fatty oxidation complex, alpha subunit FadJ (TIGR02440; EC 1.1.1.35,4.2.1.17,5.1.2.3; HMM-score: 154.8)and 10 more
- ⊞TheSEED :
- 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)
- Enoyl-CoA hydratase (EC 4.2.1.17)
- Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17)
Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)and 9 more - ⊞PFAM: NADP_Rossmann (CL0063) 3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 175)and 9 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLIAKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQQVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSHTKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVDALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGALGRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQDEAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVKTRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQLLLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHEDGLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYIGLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQTSMTKVLTNIAFAKVSTNAFEARRYGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDAQRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYERIAHMLKTGKPLRN
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- ⊟interaction partners:
SAUSA300_0845 (ampA) cytosol aminopeptidase [1] (data from MRSA252) SAUSA300_1678 (fhs) formate--tetrahydrofolate ligase [1] (data from MRSA252) SAUSA300_0993 (pdhA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) SAUSA300_1603 (rplU) 50S ribosomal protein L21 [1] (data from MRSA252) SAUSA300_2199 (rplV) 50S ribosomal protein L22 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Jump up to: 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)