PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0255 [new locus tag: SAUSA300_RS01360 ]
• pan locus tag^?: SAUPAN001140000
• symbol: SAUSA300_0255
• pan gene symbol^?: lytR
• synonym:
• product: two-component response regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0255 [new locus tag: SAUSA300_RS01360 ]
• symbol: SAUSA300_0255
• product: two-component response regulator
• replicon: chromosome
• strand: +
• coordinates: 307858..308598
• length: 741
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914555 NCBI
• RefSeq: YP_492969 NCBI
• BioCyc: see SAUSA300_RS01360
• MicrobesOnline: 1291770 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  
  ATGAAAGCATTAATCATAGATGATGAGCCATTAGCACGTAATGAATTAACATATTTATTA
  AATGAAATTGGTGGTTTTGAAGAAATTAATGAGGCAGAAAATGTAAAAGAAACATTGGAA
  GCACTACTGATCAATCAATATGACATTATATTTTTAGATGTCAATTTAATGGATGAAAAT
  GGGATCGAATTAGGAGCTAAGATTCAAAAGATGAAAGAGCCACCTGCGATTATTTTTGCA
  ACTGCACATGACCAATACGCAGTACAGGCATTTGAATTAAATGCGACAGACTATATTTTG
  AAACCGTTTGGTCAAAAACGTATTGAACAAGCAGTCAATAAAGTGCGTGCGACTAAAGCC
  AAAGATGATAATAACGCAAGTGCAATTGCGAATGATATGTCGGCGAATTTTGATCAAAGC
  TTACCTGTTGAAATTGACGATAAAATTCACATGTTAAAGCAACAAAATATTATTGGGATT
  GGCACACATAATGGTATTACAACCATACATACAACGAATCATAAATACGAAACAACAGAG
  CCATTGAATCGTTATGAAAAACGATTGAATCCCACTTATTTTATACGTATTCATCGTTCA
  TATATTATTAACACGAAACACATTAAAGAAGTGCAACAATGGTTTAACTATACTTATATG
  GTAATATTGACAAATGGTGTCAAGATGCAAGTTGGACGTTCATTTATGAAAGATTTTAAA
  GCGTCGATAGGATTACTTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  741
  

⊟Protein

⊟General

• locus tag: SAUSA300_0255 [new locus tag: SAUSA300_RS01360 ]
• symbol: SAUSA300_0255
• description: two-component response regulator
• length: 246
• theoretical pI: 5.90882
• theoretical MW: 28221.1
• GRAVY: -0.356098

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 42.9)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 41)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 41)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 41)
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 39.8)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 39.8)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 36.8)
  and 2 more
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 29.9)
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 21.5)
• TheSEED  :
  • Autolysis response regulator LytR
  Regulation and Cell signaling Programmed Cell Death and Toxin-antitoxin Systems Murein hydrolase regulation and cell death  Autolysis response regulater LytR
• PFAM:
  Tudor (CL0049) LytTR; LytTr DNA-binding domain (PF04397; HMM-score: 100.2)
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 85.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001532
  • TAT(Tat/SPI): 0.00008
  • LIPO(Sec/SPII): 0.000165
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161867 NCBI
• RefSeq: YP_492969 NCBI
• UniProt: Q2FK09 UniProt

⊟Protein sequence

• MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLINQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKPFGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGIGTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYTYMVILTNGVKMQVGRSFMKDFKASIGLL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0254 > SAUSA300_0255

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications