PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0300 [new locus tag: SAUSA300_RS01600 ]
• pan locus tag^?: SAUPAN000520000
• symbol: SAUSA300_0300
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0300 [new locus tag: SAUSA300_RS01600 ]
• symbol: SAUSA300_0300
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 351162..351662
• length: 501
• essential: unknown

⊟Accession numbers

• Gene ID: 3913867 NCBI
• RefSeq: YP_493014 NCBI
• BioCyc: see SAUSA300_RS01600
• MicrobesOnline: 1291815 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACTTTCGAAGGAAAACTAAGTCAAATGTACAACGAGATTGCGAATGAGATTAGTGGG
  ATGATACCGGTAGAATGGGAGCAAGTATTTACAATAGCTTATGTAACTGATCAAGCTGGA
  GAAGTCATCTTTAATTATACTAAACCAGATAGTGATGAATTAAATTATTATTCAGACATA
  CCTAAAGATTGCAATGTCTCAAAAGATATTTTTAAGAATTCATGGTTTAAAGTTTATCGA
  ATGTTTGATGAGTTAAGAGAAACTTTTAAAGAAGAAGGGCTTGAACCATGGACATCATGC
  GAATTTGACTTTACAAGAGATGGCAAATTGAATGTATCTTTTGATTATATAGATTGGATA
  AATACAGAGTTTGATCAATTGGGCCGTCAAAATTATTATATGTACAAAAAATTTGGGGTT
  ATACCAGAAATGGAATATGAAATGGAAGAAGTTAAAGAAATCGAACAATATATTAAAGAG
  CAAGAAGAAGCTGAACAATAG
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  501
  

⊟Protein

⊟General

• locus tag: SAUSA300_0300 [new locus tag: SAUSA300_RS01600 ]
• symbol: SAUSA300_0300
• description: hypothetical protein
• length: 166
• theoretical pI: 3.93128
• theoretical MW: 20039.2
• GRAVY: -0.735542

⊟Function

• TIGRFAM:
  conserved hypothetical protein (TIGR01741; HMM-score: 270.7)
• TheSEED  :
  • Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
  Membrane Transport Protein secretion system, Type VII ESAT-6 proteins secretion system in Firmicutes  Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
• PFAM:
  no clan defined DUF600; Protein of unknown function, DUF600 (PF04634; HMM-score: 171.4)
  and 2 more
  DUF1031; Protein of unknown function (DUF1031) (PF06275; HMM-score: 14.7)
  Holin-II (CL0563) Phage_holin_2_2; Phage holin T7 family, holin superfamily II (PF10746; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006301
  • TAT(Tat/SPI): 0.000387
  • LIPO(Sec/SPII): 0.000632
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161258 NCBI
• RefSeq: YP_493014 NCBI
• UniProt: A0A0H2XGS6 UniProt

⊟Protein sequence

• MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIFNYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEFDFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKEQEEAEQ

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0298 > SAUSA300_0299 > SAUSA300_0300 > SAUSA300_0301 > SAUSA300_0302

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications