PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0472 [new locus tag: SAUSA300_RS02535 ]
• pan locus tag^?: SAUPAN002233000
• symbol: ipk
• pan gene symbol^?: ipk
• synonym:
• product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0472 [new locus tag: SAUSA300_RS02535 ]
• symbol: ipk
• product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
• replicon: chromosome
• strand: +
• coordinates: 530846..531694
• length: 849
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913219 NCBI
• RefSeq: YP_493181 NCBI
• BioCyc: see SAUSA300_RS02535
• MicrobesOnline: 1291987 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATATATGAAACGGCACCAGCCAAAATTAATTTTACGCTCGATACACTTTTTAAAAGA
  AATGATGGCTATCATGAGATTGAAATGATAATGACAACAGTTGATTTAAATGATCGTTTA
  ACTTTTCATAAAAGAAAAGATCGAAAGATAGTTGTTGAGATTGAACATAATTATGTGCCT
  TCTAATCATAAAAATCTCGCATATCGTGCAGCGCAACTATTTATTGAGCAATATCAACTA
  AAGCAAGGTGTAACAATTTCTATCGATAAAGAAATACCTGTTTCTGCTGGCTTAGCTGGA
  GGTTCGGCTGATGCAGCAGCAACGTTAAGAGGATTGAATCGACTTTTTGATATAGGGGCG
  AGTTTGGAAGAATTGGCTCTACTAGGCAGTAAAATCGGGACAGATATTCCGTTTTGTATT
  TATAATAAAACTGCACTATGTACTGGAAGAGGAGAGAAAATCGAGTTTTTAAATAAACCA
  CCTTCAGCTTGGGTGATTCTTGCTAAACCAAACTTAGGCATATCATCACCAGATATATTT
  AAGTTGATTAATTTAGATAAGCGTTACGACGTACATACGAAAATGTGTTATGAGGCCTTA
  GAAAATCGAGATTATCAACAATTATGTCAAAGTTTGTCTAATCGATTAGAGCCAATTTCT
  GTTTCAAAACACCCACAAATCGATAAATTAAAAAATAATATGTTGAAAAGTGGTGCAGAT
  GGTGCGTTAATGAGTGGAAGCGGACCTACTGTGTATGGGCTAGCACGAAAAGAAAGCCAA
  GCAAAAAATATTTATAATGCAGTTAACGGTTGTTGTAATGAAGTGTACTTAGTTAGACTA
  TTAGGATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_0472 [new locus tag: SAUSA300_RS02535 ]
• symbol: Ipk
• description: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
• length: 282
• theoretical pI: 8.54156
• theoretical MW: 31442
• GRAVY: -0.251064

⊟Function

• reaction:
  EC 2.7.1.148?  ExPASy
  4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 297.4)
  and 5 more
  Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 52.3)
  Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 39)
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 35.2)
  Unknown function Enzymes of unknown specificity beta-ribofuranosylaminobenzene 5'-phosphate synthase family (TIGR00144; HMM-score: 13.9)
  Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 12.3)
• TheSEED  :
  • Diphosphocytidyl-sugar kinase
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148)
• PFAM:
  no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 44.5)
  S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 44.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001934
  • TAT(Tat/SPI): 0.000105
  • LIPO(Sec/SPII): 0.000429
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161506 NCBI
• RefSeq: YP_493181 NCBI
• UniProt: Q2FJE7 UniProt

⊟Protein sequence

• MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHKRKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGSADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKPPSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEPISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVYLVRLLG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ipk > purR > SAUSA300_0474 > SAUSA300_0475

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications