PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0558 [new locus tag: SAUSA300_RS02975 ]
• pan locus tag^?: SAUPAN002350000
• symbol: SAUSA300_0558
• pan gene symbol^?: proP
• synonym:
• product: putative proline/betaine transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0558 [new locus tag: SAUSA300_RS02975 ]
• symbol: SAUSA300_0558
• product: putative proline/betaine transporter
• replicon: chromosome
• strand: +
• coordinates: 631334..632734
• length: 1401
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914545 NCBI
• RefSeq: YP_493261 NCBI
• BioCyc: see SAUSA300_RS02975
• MicrobesOnline: 1292073 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGATTTTAATAAAGAGAATATTAACATGGTGGATGCAAAGAAAGCTAAAAAAACCGTT
  GTTGCAACCGGTATCGGTAATGCAATGGAATGGTTCGATTTTGGTGTCTATGCATATACA
  ACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATG
  TTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTT
  GGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATG
  GCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCA
  CCAATACTATTATTGCTTGCAAGAGTACTACAAGGGTTTTCAACAGGTGGAGAGTATGCG
  GGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGT
  GGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTA
  ACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTC
  GGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTT
  TTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATC
  AGATTTTATTACAAAGATATATTTGTATGTTTTGTAGCTGTTGTATTCTTCAATGTTACA
  AACTATATGGTAACTGCATATTTACCAACCTATTTAGAACAAGTTATTAAATTAGATGCA
  ACGACAACAAGTGTATTAATTACTTGTGTCATGGCAATAATGATTCCATTAGCATTAATG
  TTTGGTAAGTTAGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGG
  CTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATA
  GTAATCGGTATATTTATATTAGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGG
  TCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAAT
  ATCTCTGTTTCGATATTTGGTGGTACGACGCCATTAGTTGCAACATGGTTAGTTACGAAA
  ACTGGAGATCCATTAGCACCTGCGTATTATTTAACAGCAATCAGTGTTATTGGCTTTTTA
  GTTATTACATTCTTACATTTAAGTACAGCAGGAAAATCTCTAAAAGGTTCGTATCCAAAT
  GTAGATAACGAGCAAGATAGAGCTTATTATGCAGAACATCCAAAAGAAGCATTATGGTGG
  GTTAAAGAACGTAAGAATTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_0558 [new locus tag: SAUSA300_RS02975 ]
• symbol: SAUSA300_0558
• description: putative proline/betaine transporter
• length: 466
• theoretical pI: 8.48542
• theoretical MW: 51657.3
• GRAVY: 0.524678

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 383.6)
  and 13 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 105)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 78)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 51.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 49.3)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 44.9)
  multidrug resistance protein (TIGR00880; HMM-score: 42.1)
  Metabolism Transport and binding proteins Anions phosphate:H+ symporter (TIGR00887; HMM-score: 35.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sugar porter (SP) family (TIGR00879; HMM-score: 32.3)
  Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 28.1)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 23.9)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 22.3)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 20.7)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 20.7)
• TheSEED  :
  • L-Proline/Glycine betaine transporter ProP
  Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization  L-Proline/Glycine betaine transporter ProP
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 114.4)
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 108.1)
  and 2 more
  MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 36.7)
  no clan defined Prominin; Prominin (PF05478; HMM-score: 4.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01901
  • TAT(Tat/SPI): 0.00033
  • LIPO(Sec/SPII): 0.000915
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 87160309 NCBI
• RefSeq: YP_493261 NCBI
• UniProt: A0A0H2XGD5 UniProt

⊟Protein sequence

• MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYIGANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAFSTLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGSGLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEESPVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVIKLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHSQSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPLVATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYYAEHPKEALWWVKERKN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for COL, NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications