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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00556
  • pan locus tag?: SAUPAN002350000
  • symbol: SAOUHSC_00556
  • pan gene symbol?: proP
  • synonym:
  • product: proline/betaine transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00556
  • symbol: SAOUHSC_00556
  • product: proline/betaine transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 564128..565528
  • length: 1401
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGATTTTAATAAAGAGAATATTAACATGGTGGATGCAAAGAAAGCTAAAAAAACCGTT
    GTTGCAACCGGTATCGGTAATGCAATGGAATGGTTCGATTTTGGTGTCTATGCATATACA
    ACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATG
    TTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTT
    GGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATG
    GCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCA
    CCAATACTATTATTGCTTGCAAGAGTACTACAAGGGTTTTCAACAGGTGGAGAGTATGCG
    GGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGT
    GGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTA
    ACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTC
    GGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTT
    TTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATC
    AGATTTTATTACAAAGATATATTTGTATGTTTTGTAGCTGTTGTATTCTTCAATGTTACA
    AACTATATGGTAACTGCATATTTACCAACCTATTTAGAACAAGTTATTAAATTAGATGCA
    ACGACAACAAGTGTATTAATTACTTGTGTCATGGCAATAATGATTCCATTAGCATTAATG
    TTTGGTAAGTTAGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGG
    CTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATA
    GTAATCGGTATATTTATATTAGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGG
    TCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAAT
    ATCTCTGTTTCGATATTTGGTGGTACGACGCCATTAGTTGCAACATGGTTAGTTACGAAA
    ACTGGAGATCCATTAGCACCTGCGTATTATTTAACAGCAATCAGTGTTATTGGCTTTTTA
    GTTATTACATTCTTACATTTAAGTACAGCAGGAAAATCTCTAAAAGGTTCGTATCCAAAT
    GTAGATAACGAGCAAGATAGAGCTTATTATGCAGAACATCCAAAAGAAGCATTATGGTGG
    GTTAAAGAACGTAAGAATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1401

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_00556
  • symbol: SAOUHSC_00556
  • description: proline/betaine transporter
  • length: 466
  • theoretical pI: 8.48542
  • theoretical MW: 51657.3
  • GRAVY: 0.524678

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 383.6)
    and 13 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 105)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 78)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 51.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 49.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 44.9)
    multidrug resistance protein (TIGR00880; HMM-score: 42.1)
    Metabolism Transport and binding proteins Anions phosphate:H+ symporter (TIGR00887; HMM-score: 35.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sugar porter (SP) family (TIGR00879; HMM-score: 32.3)
    Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 28.1)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 23.9)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 22.3)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 20.7)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 20.7)
  • TheSEED:  
    Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization  L-Proline/Glycine betaine transporter ProP
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 114.4)
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 108.1)
    and 2 more
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 36.7)
    no clan defined Prominin; Prominin (PF05478; HMM-score: 4.6)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01901
    • TAT(Tat/SPI): 0.00033
    • LIPO(Sec/SPII): 0.000915
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYIGANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAFSTLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGSGLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEESPVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVIKLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHSQSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPLVATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYYAEHPKEALWWVKERKN

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • SAOUHSC_00556 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [3]   other strains

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]