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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0589 [new locus tag: SAUSA300_RS03165 ]
- pan locus tag?: SAUPAN002463000
- symbol: SAUSA300_0589
- pan gene symbol?: iolS
- synonym:
- product: aldo/keto reductase family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0589 [new locus tag: SAUSA300_RS03165 ]
- symbol: SAUSA300_0589
- product: aldo/keto reductase family protein
- replicon: chromosome
- strand: +
- coordinates: 662761..663699
- length: 939
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3912767 NCBI
- RefSeq: YP_493292 NCBI
- BioCyc: see SAUSA300_RS03165
- MicrobesOnline: 1292104 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGAAGCAATTTGTAAACTTAGGTAAATCTGATGTTGAAGTGTTTCCAATCGCACTTGGG
ACGAACGCAGTAGGTGGGCATAATTTATATCCGAACTTAGATGAAGAACAAGGAAAAGAT
GTTGTTCGTCAAGCCATTAATCATGGTATTAATTTATTAGATACGGCATATATTTATGGG
CCAGAACGATCAGAAGAATTGGTTGGAGAAGTTGTTAAAGAATATCCGCGAGAGCAAATT
AAAATTGCTACGAAAGGGTCTCATGAATTTGATGAAAATCAAGAAGTACATCAGAACAAT
CAACCGGAATATTTAAAACAACAAGTTGAGAATAGTTTGAAACGTCTACAAACTGATTAT
ATCGATTTATATTATATTCATTTTCCGGATAACAACACTCCGAAAGATCAAGCAGTTGCA
GCATTACAAGAGCTTAAGGAACAAGGGAAGATTAAAGCAATTGGTGTATCAAATTTCACA
TTAGATCAACTTAAAGAAGCAAATAAAGATGGTTACGTTGATGTTGTACAGTTAGAATAT
AATTTATTGCATCGCGAAAATGAGGCAGTATTACAATATTGTGTTGATCACCAAATCACA
TTTATTCCATATTTCCCATTAGCATCCGGTATTTTAGCTGGAAAATATGATGAGAACACT
AAATTTAGTGACCATCGTACTACACGTCGTGATTTTAAACCAGGTGTATTTGAAGAAAAT
GTGCGTCGCGTAAAAGCTTTGGAAAGCATAGCTGCAGCACATCAAACTTCAATTGCGAAC
ATTGTATTAGCATTTTATTTAACGAGACCAGCTATCGATGTGATTATTCCTGGTGCAAAA
CGTGCAGAACAAGTCGTTGAAAATATTAAAGCTGCAGATATCGTTTTATCAGATGATGAG
ATTCAATATATCGATGAACTGTTTCCGATTGAAGACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0589 [new locus tag: SAUSA300_RS03165 ]
- symbol: SAUSA300_0589
- description: aldo/keto reductase family protein
- length: 312
- theoretical pI: 4.6314
- theoretical MW: 35459.5
- GRAVY: -0.465385
⊟Function[edit | edit source]
- TIGRFAM: voltage-dependent potassium channel beta subunit (TIGR01293; HMM-score: 90.4)
- TheSEED :
- Oxidoreductase ion channel
- PFAM: no clan defined Aldo_ket_red; Aldo/keto reductase family (PF00248; HMM-score: 255.8)and 3 moreNADP_Rossmann (CL0063) Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 16.3)HAD (CL0137) Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 13.7)no clan defined RasGAP_C; RasGAP C-terminus (PF03836; HMM-score: 13)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.01299
- TAT(Tat/SPI): 0.000682
- LIPO(Sec/SPII): 0.003841
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQAINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQPEYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFTLDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDENTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVIIPGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_1201 (glnA) glutamine synthetase, type I [1] (data from MRSA252) SAUSA300_1459 (gnd) 6-phosphogluconate dehydrogenase [1] (data from MRSA252) SAUSA300_1162 (infB) translation initiation factor IF-2 [1] (data from MRSA252) SAUSA300_0993 (pdhA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) SAUSA300_2203 (rplD) 50S ribosomal protein L4 [1] (data from MRSA252) SAUSA300_2192 (rplE) 50S ribosomal protein L5 [1] (data from MRSA252) SAUSA300_0524 (rplJ) 50S ribosomal protein L10 [1] (data from MRSA252) SAUSA300_2177 (rplQ) 50S ribosomal protein L17 [1] (data from MRSA252) SAUSA300_2202 (rplW) 50S ribosomal protein L23 [1] (data from MRSA252) SAUSA300_1601 (rpmA) 50S ribosomal protein L27 [1] (data from MRSA252) SAUSA300_1626 (rpmI) 50S ribosomal protein L35 [1] (data from MRSA252) SAUSA300_0528 (rpoC) DNA-directed RNA polymerase subunit beta' [1] (data from MRSA252) SAUSA300_1149 (rpsB) 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_2198 (rpsC) 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_2187 (rpsE) 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_0366 (rpsF) 30S ribosomal protein S6 [1] (data from MRSA252) SAUSA300_0368 (rpsR) 30S ribosomal protein S18 [1] (data from MRSA252) SAUSA300_1044 (trx) thioredoxin [1] (data from MRSA252) SAUSA300_1150 (tsf) elongation factor Ts [1] (data from MRSA252) SAUSA300_2066 (upp) uracil phosphoribosyltransferase [1] (data from MRSA252) SAUSA300_0113 immunoglobulin G binding protein A [1] (data from MRSA252) SAUSA300_0235 L-lactate dehydrogenase [1] (data from MRSA252) SAUSA300_1513 Fe/Mn family superoxide dismutase [1] (data from MRSA252) SAUSA300_1656 universal stress protein [1] (data from MRSA252) SAUSA300_2540 fructose-1,6-bisphosphate aldolase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)