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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0714 [new locus tag: SAUSA300_RS03835 ]
  • pan locus tag?: SAUPAN002627000
  • symbol: SAUSA300_0714
  • pan gene symbol?: —
  • synonym:
  • product: integral membrane protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0714 [new locus tag: SAUSA300_RS03835 ]
  • symbol: SAUSA300_0714
  • product: integral membrane protein
  • replicon: chromosome
  • strand: -
  • coordinates: 792106..792972
  • length: 867
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    TTGAATCCTAAAGTAAAAGGTATCATTGCCATTTTAATTTCGGCTATTGGTTTCAGTTTT
    ATGTCAGTGTTTTTCAGACTTGCAGGTGACCTTCCTGTTTTTCAAAAATCATTAGCACGA
    AACTTAGTAGCAATGTTTATTCCATTATTTTTTATATATAAATACCATCAGCCAATGTTT
    GGCAGACTTTCAAGTCAACCCTTATTAATAACACGATCAACACTTGGACTTATTGGTGTA
    TTGTTAAATATTTATGCCATTGATCATATGGTACTTAGTGATGCCGATTCATTAATGAAA
    CTCAATCCTTTCTGGACGATTTTATTAAGCATTGTTTTCTTACATGAAAAGGTAAGGAAG
    TATCAAATTACAGCAATGATAATTGCTATTTTAGGTATGCTTTTAATAGTGAAACCTGAA
    TTTTCATCATCTATGATTCCATCATTAGCTGGCTTATTCTCAGGTATTTTTGCAGCATCA
    GCATATACATGTGTTCGAGCACTTAGTACAAGAGAAGCACCATATACTATAGTATTTTAC
    TTCTCCTTATTTTCTGTCATCGTTTTAATACCTTTCACTGCATATACTTATGAACCAATG
    AGTCAGATGCAAATACTTTATCTATTAGGGGCTGGCTTAGCTGCCGCTGTGGGTCAAATT
    GGTGTTACTTTAGCATATAGTTTTGCAGCAGCAAAAGATATTTCTATTTTTACGTATGCA
    TCTATAATTTTCACAGCAATACTTGGATTTATTCTATTCGGAGAATCTCCAGATTTCTAT
    GCAACATTAGGTTACGTCGTTATTATCGGTGCAAGTTATTACATGTTTGAAAAAGCACGC
    CGAGACGCCAAAATAATAAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    867

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0714 [new locus tag: SAUSA300_RS03835 ]
  • symbol: SAUSA300_0714
  • description: integral membrane protein
  • length: 288
  • theoretical pI: 9.85057
  • theoretical MW: 31954.1
  • GRAVY: 0.866667

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines carboxylate/amino acid/amine transporter (TIGR00950; HMM-score: 40.1)
    and 2 more
    Metabolism Transport and binding proteins Unknown substrate protein RarD (TIGR00688; HMM-score: 19.7)
    phosphonate utilization associated putative membrane protein (TIGR03340; HMM-score: 14.2)
  • TheSEED  :
    • Integral membrane domain protein
  • PFAM:
    DMT (CL0184) EamA; EamA-like transporter family (PF00892; HMM-score: 82.8)
    and 2 more
    TPT; Triose-phosphate Transporter family (PF03151; HMM-score: 21.9)
    Mg_trans_NIPA; Magnesium transporter NIPA (PF05653; HMM-score: 20.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.030589
    • TAT(Tat/SPI): 0.001735
    • LIPO(Sec/SPII): 0.009224
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVAMFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLNPFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAASAYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVGQIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMFEKARRDAKIIKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LexA (repression) regulon
    LexA(TF)important in SOS response; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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