From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0759 [new locus tag: SAUSA300_RS04095 ]
  • pan locus tag?: SAUPAN002709000
  • symbol: gpmI
  • pan gene symbol?: pgm
  • synonym:
  • product: phosphoglyceromutase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0759 [new locus tag: SAUSA300_RS04095 ]
  • symbol: gpmI
  • product: phosphoglyceromutase
  • replicon: chromosome
  • strand: +
  • coordinates: 846572..848089
  • length: 1518
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCTAAGAAACCAACTGCGTTAATTATTTTAGATGGTTTTGCGAACCGCGAAAGCGAA
    CATGGTAATGCGGTAAAATTAGCAAACAAGCCTAATTTTGATCGTTATTACAACAAATAT
    CCAACGACTCAAATCGAAGCGAGTGGCTTAGATGTTGGACTACCTGAAGGACAAATGGGT
    AACTCAGAAGTTGGTCATATGAATATCGGTGCAGGACGTATCGTTTATCAAAGTTTAACT
    CGAATCAATAAATCAATTGAAGACGGTGATTTCTTTGAAAATGATGTTTTAAATAATGCA
    ATTGCACACGTGAATTCACATGATTCAGCGTTACACATCTTTGGTTTATTGTCTGACGGT
    GGTGTACACAGTCATTACAAACATTTATTTGCTTTGTTAGAACTTGCTAAAAAACAAGGT
    GTTGAAAAAGTTTACGTACACGCATTTTTAGATGGCCGTGACGTAGATCAAAAATCCGCT
    TTGAAATACATCGAAGAGACTGAAGCTAAATTCAATGAATTAGGCATTGGTCAATTTGCA
    TCTGTGTCTGGTCGTTATTATGCAATGGATCGTGACAAACGTTGGGAACGTGAAGAAAAA
    GCTTACAATGCTATTCGTAATTTTGATGCCCCAACTTATGCAACTGCCAAAGAAGGTGTA
    GAAGCAAGCTATAATGAGGGCTTAACTGACGAATTCGTAGTACCATTCATCGTTGAGAAT
    CAAAATGACGGTGTTAATGATGGAGATGCAGTGATCTTCTATAATTTCCGACCTGATAGA
    GCAGCGCAATTATCGGAAATTTTTGCGAACAGAGCATTCGAAGGCTTTAAAGTTGAACAA
    GTTAAAGACTTATTCTATGCAACATTCACTAAGTATAATGACAATATCGATGCGGCTATC
    GTCTTCGAAAAAGTTGATTTAAATAATACAATTGGTGAAATTGCACAAAATAACAATTTA
    ACTCAATTACGTATTGCAGAAACTGAAAAATACCCTCACGTTACTTACTTTATGAGTGGT
    GGACGTAACGAGGAATTTAAAGGTGAACGCCGTCGTTTAATTGATTCACCTAAAGTTGCA
    ACGTATGACTTGAAACCAGAAATGAGTGCTTATGAAGTTAAAGATGCATTATTAGAAGAG
    TTAAATAAAGGTGACTTGGACTTAATTATTTTAAACTTTGCTAACCCTGATATGGTTGGA
    CATAGTGGTATGCTTGAGCCGACAATCAAAGCAATCGAAGCGGTTGATGAATGTTTAGGA
    GAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGT
    AACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCA
    GTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGAC
    TTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGATATGACAGGTGAA
    TCTTTAATTAAACACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1518

⊟Protein[edit | edit source]

Protein Data Bank: 4MY4
Protein Data Bank: 4NWJ
Protein Data Bank: 4NWX
Protein Data Bank: 4QAX

⊟General[edit | edit source]

  • locus tag: SAUSA300_0759 [new locus tag: SAUSA300_RS04095 ]
  • symbol: GpmI
  • description: phosphoglyceromutase
  • length: 505
  • theoretical pI: 4.49265
  • theoretical MW: 56423.7
  • GRAVY: -0.427327

⊟Function[edit | edit source]

  • reaction:
    EC 5.4.2.1?  ExPASy
    Transferred entry: 5.4.2.11 and 5.4.2.12
    EC 5.4.2.12?  ExPASy
    Phosphoglycerate mutase (2,3-diphosphoglycerate-independent) 2-phospho-D-glycerate = 3-phospho-D-glycerate
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoglycerate mutase (2,3-diphosphoglycerate-independent) (TIGR01307; EC 5.4.2.12; HMM-score: 694.5)
    and 2 more
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoglycerate mutase (2,3-diphosphoglycerate-independent), archaeal form (TIGR00306; EC 5.4.2.12; HMM-score: 51.7)
    proposed homoserine kinase (TIGR02535; HMM-score: 28.2)
  • TheSEED  :
    • 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.12)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1)
  • PFAM:
    no clan defined iPGM_N; BPG-independent PGAM N-terminus (iPGM_N) (PF06415; HMM-score: 278.5)
    Alk_phosphatase (CL0088) Metalloenzyme; Metalloenzyme superfamily (PF01676; HMM-score: 257.5)
    and 3 more
    Phosphodiest; Type I phosphodiesterase / nucleotide pyrophosphatase (PF01663; HMM-score: 22.8)
    PglZ; PglZ domain (PF08665; HMM-score: 17.2)
    Sulfatase; Sulfatase (PF00884; HMM-score: 16.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.036797
    • TAT(Tat/SPI): 0.001171
    • LIPO(Sec/SPII): 0.003382
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQIEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIAHVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSALKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKEGVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGFKVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHVTYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILNFANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDDQPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: GapR* (repression) regulon
    GapR*(TF)important in Glycolysis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_0759&oldid=54406"