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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0757 [new locus tag: SAUSA300_RS04085 ]
  • pan locus tag?: SAUPAN002707000
  • symbol: pgk
  • pan gene symbol?: pgk
  • synonym:
  • product: phosphoglycerate kinase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0757 [new locus tag: SAUSA300_RS04085 ]
  • symbol: pgk
  • product: phosphoglycerate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 844496..845686
  • length: 1191
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCTAAAAAAATTGTTTCTGATTTAGATCTTAAAGGTAAAACAGTCCTAGTACGTGCT
    GATTTTAACGTACCTTTAAAAGACGGTGAAATTACTAATGACAACCGTATCGTTCAAGCT
    TTACCTACAATTCAATACATCATCGAACAAGGTGGTAAAATCGTACTATTTTCACATTTA
    GGTAAAGTGAAAGAAGAAAGTGATAAAGCAAAATTAACTTTACGTCCAGTTGCTGAAGAC
    TTATCTAAGAAATTAGATAAAGAAGTTGTTTTCGTACCAGAAACACGCGGCGAAAAACTT
    GAAGCTGCTATTAAAGACCTTAAAGAAGGCGACGTATTATTAGTTGAAAATACACGTTAT
    GAAGATTTAGACGGTAAAAAAGAATCTAAAAATGATCCAGAATTAGGTAAATACTGGGCA
    TCTTTAGGTGATGTGTTTGTAAATGATGCTTTTGGTACTGCGCATCGTGAGCATGCATCT
    AATGTTGGTATTTCTACACATTTAGAAACTGCAGCTGGATTCTTAATGGATAAAGAAATT
    AAGTTTATTGGCGGCGTAGTTAACGATCCACATAAACCAGTTGTTGCTATTTTAGGTGGA
    GCAAAAGTATCTGACAAAATTAATGTCATCAAAAACTTAGTTAACATAGCTGATAAAATT
    ATCATCGGCGGAGGTATGGCTTATACTTTCTTAAAAGCGCAAGGTAAAGAAATTGGTATT
    TCATTATTAGAAGAAGATAAAATCGACTTCGCAAAAGATTTATTAGAAAAACATGGTGAT
    AAAATTGTATTACCAGTAGACACTAAAGTTGCTAAAGAATTTTCTAATGATGCCAAAATC
    ACTGTAGTACCATCTGATTCAATTCCAGCAGACCAAGAAGGTATGGATATTGGACCAAAC
    ACTGTAAAATTATTTGCAGATGAATTAGAAGGTGCGCACACTGTTGTATGGAATGGACCT
    ATGGGTGTATTCGAGTTCAGTAACTTTGCACAAGGTACAATTGGTGTATGTAAAGCAATT
    GCAAACCTTAAAGATGCAATTACGATTATCGGTGGCGGTGATTCAGCTGCAGCAGCAATC
    TCTTTAGGTTTTGAAAATGACTTCACTCATATTTCAACTGGTGGCGGCGCGTCATTAGAG
    TACCTAGAAGGTAAAGAATTGCCTGGTATCAAAGCAATCAATAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1191

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0757 [new locus tag: SAUSA300_RS04085 ]
  • symbol: Pgk
  • description: phosphoglycerate kinase
  • length: 396
  • theoretical pI: 4.96143
  • theoretical MW: 42601.4
  • GRAVY: -0.126263

Function[edit | edit source]

  • reaction:
    EC 2.7.2.3?  ExPASy
    Phosphoglycerate kinase ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
  • TIGRFAM:
  • TheSEED  :
    • Phosphoglycerate kinase (EC 2.7.2.3)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  Phosphoglycerate kinase (EC 2.7.2.3)
  • PFAM:
    no clan defined PGK; Phosphoglycerate kinase (PF00162; HMM-score: 515.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002366
    • TAT(Tat/SPI): 0.000092
    • LIPO(Sec/SPII): 0.000395
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTIQYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEAAIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHASNVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIADKIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSNDAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTIGVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKAINNK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_1880(gatB)aspartyl/glutamyl-tRNA amidotransferase subunit B  [1] (data from MRSA252)
    SAUSA300_0996(lpdA)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SAUSA300_0993(pdhA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    SAUSA300_0994(pdhB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    SAUSA300_2171(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    SAUSA300_0995branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: GapR* (repression) regulon
    GapR*(TF)important in Glycolysis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]