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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0809 [new locus tag: SAUSA300_RS04365 ]
  • pan locus tag?: SAUPAN002857000
  • symbol: SAUSA300_0809
  • pan gene symbol?: —
  • synonym:
  • product: putative DNA primase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0809 [new locus tag: SAUSA300_RS04365 ]
  • symbol: SAUSA300_0809
  • product: putative DNA primase
  • replicon: chromosome
  • strand: +
  • coordinates: 887405..889777
  • length: 2373
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    GTGGCAATTAAGAAAAAAGATAGAATTATTGGAGTTAAAGAATTAGAAATACCGCAAGAA
    TTAAAATTAGTGCCCAATTGGGTATTATGGCGAGCTGAATGGAACGAGAAGCAACAAAAT
    TTTGGAAAAGTACCATATAGTATTAATGGTTACAGAGCTAGTACAACCAATAAAAAAACA
    TGGTGTGACTTTGAAAGTGTAAGTATTGAATATGAAGTTGATGAGCAATATAGCGGTATA
    GGTTTTGTATTAAGTGATGGTAATAATTTTGTTTGCCTAGATATTGATAATGCAATTGAT
    AAAAAAGGACAAATCAATTCTGAATTAGCACTAAAAATGATGCAACTCACATATTGTGAA
    AAGTCTCCAAGTGGTACAGGATTACATTGTTTCTTTAAAGGTAAACTACCAGATAACCGT
    AAAAAGAAAAGAACAGATTTAGACATAGAGTTATATGATTCAGCAAGGTTTATGACTGTT
    ACAGGATGCACAATTGGTCAAAGTGATATTTGTGATAATCAAGAAGTATTAAATACTCTA
    GTTGATGAATATTTTAAAGAGAATTTGCCAGCAAATGAGGTTGTGAGAGAGGAATCTAAT
    ACCAATATACAATTATCTGATGAAGATATTATAAACATTATGATGAAATCTAAACAAAAA
    GATAAAATTAAAGATCTATTACAAGGCACATATGAATCATATTTTGAGAGTTCAAGCGAA
    GCAGTACAAAGCTTATTACACTACTTAGCGTTTTACACAGGTAAAAACAAACAGCAAATG
    GAGCGTATATTTTTAAACTACAATAATCTTACGGATAAATGGGAAAGTAAACGAGGTAAT
    ACGACTTGGGGACAGCTTGAGTTAGATAAAGCTATAAAGAATCAAAAGACAATTTATACT
    AAATCGATAGATGAATTTAATGTTATACCACAGGGGAGTAAAGATGTTAAACAGTTGTTG
    AATCAATTGGGGCATGAAGAAAGAACAAAAATGGAAGAAAATTGGATTGAAGAAGGAAAA
    CGAGGGCGAAAGCCTACAACAATTAGCCCTATAAAATGTGCATATATTTTGAATGAGCAT
    TTAACATTTATACTTTTTGATGATGAAGAAAATACTAAGTTAGCTATGTATCAATTTGAT
    GAAGGGATATATACACAGAACACTACAATTATAAAACGAGTGATTTCCTATTTAGAGCCC
    AAACATAATAGCAACAAAGCTGATGAAGTTATTTATCATTTAACCAATATGGTAGATATA
    AAAGAGAAAACTAACTCACCATACTTAATACCAGTTAAAAATGGTGTATTTAACCGTAAA
    ACGAAGCAACTAGAATCATTTACACCTGATTATATATTTACCAGTAAAATAGATACATCG
    TATGTAAGGCAAGATATAGTACCTGAAATAAATGGCTGGAATATAGATCGGTGGATAGAA
    GAGATTGCTTGTAATGATAATCAGGTTGTTAAATTATTGTGGCAAGTAATTAATGACTCA
    ATGAATGGTAACTACACTCGTAAAAAGGCTATTTTCTTTGTTGGTGATGGAAATAATGGT
    AAAGGGACGTTTCAAGAATTATTGTCTAATGTAATAGGTTATAGCAATATTGCTAGCTTA
    AAAGTGAATGAGTTTGATGAACGTTTTAAATTGAGTGTGTTAGAGGGCAAGACAGCAGTA
    ATTGGAGACGATGTACCAGTTGGTGTGTATGTCGATGATTCTTCAAACTTTAAAAGTGTA
    GTTACTGGCGATCCAGTGTTGGTTGAGTTTAAAAATAAACCCTTATATAGAGCGACTTTT
    AAGTGTACAGTTATTCAATCAACGAATGGAATGCCTAAATTTAAAGACAAAACAGGCGGG
    ACTTTAAGAAGGTTATTGATAGTACCGTTTAATGCCAATTTTAATGGCATTAAAGAGAAT
    TTTAAAATCAAAGAAGACTATATAAAAAATCAGCAAGTGCTAGAGTATGTGCTTTATAAA
    GCAATTAATTTAGATTTTGAAACCTTTGACATTCCTGATGCATCTAAAAAAATGCTTGAA
    GTATTTAAAGAAGATAACGATCCAGTTTATGGGTTTAAAGTAAATATGTTTGATCAATGG
    ACTATTAGAAAAGTGCCGAAATATATTGTATACGCATTTTATAAAGAATATTGTGATGAA
    AATGGCTATAATGCATTGAGTTCAAACAAGTTTTATAAACAATTTGAACATTATTTAGAG
    AATTATTGGAAAACTGATGCACAGCGAAGATATGACAATGAAGAACTTGCTAAGAGGATA
    TACAACTTTAATGACAATAGAAATTACATTGAACCTATTGAAAGTGGAAAAAACTATAAA
    TCGTATGAAAAGGTGAAGCTAAAAGCAATATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2373

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0809 [new locus tag: SAUSA300_RS04365 ]
  • symbol: SAUSA300_0809
  • description: putative DNA primase
  • length: 790
  • theoretical pI: 6.08403
  • theoretical MW: 92004.7
  • GRAVY: -0.631646

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair phage/plasmid primase, P4 family, C-terminal domain (TIGR01613; HMM-score: 370.7)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage/plasmid primase, P4 family, C-terminal domain (TIGR01613; HMM-score: 370.7)
    and 1 more
    HipA N-terminal domain (TIGR03071; HMM-score: 14.1)
  • TheSEED  :
    • Putative primase, superantigen-encoding pathogenicity islands SaPI
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Putative primase, superantigen-encoding pathogenicity islands SaPI
  • PFAM:
    HTH (CL0123) Pox_D5; Poxvirus D5 protein-like (PF03288; HMM-score: 54.3)
    and 2 more
    no clan defined D5_N; D5 N terminal like (PF08706; HMM-score: 39.9)
    HTH (CL0123) RFX_DNA_binding; RFX DNA-binding domain (PF02257; HMM-score: 16.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008251
    • TAT(Tat/SPI): 0.000748
    • LIPO(Sec/SPII): 0.000885
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKVPYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKKGQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTVTGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSKQKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWESKRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEENWIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIKRVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPDYIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRKKAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVPVGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRRLLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVFKEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLENYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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