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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS04365 [old locus tag: SAUSA300_0809 ]
- pan locus tag?: SAUPAN002857000
- symbol: SAUSA300_RS04365
- pan gene symbol?: —
- synonym:
- product: primase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS04365 [old locus tag: SAUSA300_0809 ]
- symbol: SAUSA300_RS04365
- product: primase
- replicon: chromosome
- strand: +
- coordinates: 887405..889777
- length: 2373
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (887405..889777) NCBI
- BioCyc: SAUSA300_RS04365 BioCyc
- MicrobesOnline: see SAUSA300_0809
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2341GTGGCAATTAAGAAAAAAGATAGAATTATTGGAGTTAAAGAATTAGAAATACCGCAAGAA
TTAAAATTAGTGCCCAATTGGGTATTATGGCGAGCTGAATGGAACGAGAAGCAACAAAAT
TTTGGAAAAGTACCATATAGTATTAATGGTTACAGAGCTAGTACAACCAATAAAAAAACA
TGGTGTGACTTTGAAAGTGTAAGTATTGAATATGAAGTTGATGAGCAATATAGCGGTATA
GGTTTTGTATTAAGTGATGGTAATAATTTTGTTTGCCTAGATATTGATAATGCAATTGAT
AAAAAAGGACAAATCAATTCTGAATTAGCACTAAAAATGATGCAACTCACATATTGTGAA
AAGTCTCCAAGTGGTACAGGATTACATTGTTTCTTTAAAGGTAAACTACCAGATAACCGT
AAAAAGAAAAGAACAGATTTAGACATAGAGTTATATGATTCAGCAAGGTTTATGACTGTT
ACAGGATGCACAATTGGTCAAAGTGATATTTGTGATAATCAAGAAGTATTAAATACTCTA
GTTGATGAATATTTTAAAGAGAATTTGCCAGCAAATGAGGTTGTGAGAGAGGAATCTAAT
ACCAATATACAATTATCTGATGAAGATATTATAAACATTATGATGAAATCTAAACAAAAA
GATAAAATTAAAGATCTATTACAAGGCACATATGAATCATATTTTGAGAGTTCAAGCGAA
GCAGTACAAAGCTTATTACACTACTTAGCGTTTTACACAGGTAAAAACAAACAGCAAATG
GAGCGTATATTTTTAAACTACAATAATCTTACGGATAAATGGGAAAGTAAACGAGGTAAT
ACGACTTGGGGACAGCTTGAGTTAGATAAAGCTATAAAGAATCAAAAGACAATTTATACT
AAATCGATAGATGAATTTAATGTTATACCACAGGGGAGTAAAGATGTTAAACAGTTGTTG
AATCAATTGGGGCATGAAGAAAGAACAAAAATGGAAGAAAATTGGATTGAAGAAGGAAAA
CGAGGGCGAAAGCCTACAACAATTAGCCCTATAAAATGTGCATATATTTTGAATGAGCAT
TTAACATTTATACTTTTTGATGATGAAGAAAATACTAAGTTAGCTATGTATCAATTTGAT
GAAGGGATATATACACAGAACACTACAATTATAAAACGAGTGATTTCCTATTTAGAGCCC
AAACATAATAGCAACAAAGCTGATGAAGTTATTTATCATTTAACCAATATGGTAGATATA
AAAGAGAAAACTAACTCACCATACTTAATACCAGTTAAAAATGGTGTATTTAACCGTAAA
ACGAAGCAACTAGAATCATTTACACCTGATTATATATTTACCAGTAAAATAGATACATCG
TATGTAAGGCAAGATATAGTACCTGAAATAAATGGCTGGAATATAGATCGGTGGATAGAA
GAGATTGCTTGTAATGATAATCAGGTTGTTAAATTATTGTGGCAAGTAATTAATGACTCA
ATGAATGGTAACTACACTCGTAAAAAGGCTATTTTCTTTGTTGGTGATGGAAATAATGGT
AAAGGGACGTTTCAAGAATTATTGTCTAATGTAATAGGTTATAGCAATATTGCTAGCTTA
AAAGTGAATGAGTTTGATGAACGTTTTAAATTGAGTGTGTTAGAGGGCAAGACAGCAGTA
ATTGGAGACGATGTACCAGTTGGTGTGTATGTCGATGATTCTTCAAACTTTAAAAGTGTA
GTTACTGGCGATCCAGTGTTGGTTGAGTTTAAAAATAAACCCTTATATAGAGCGACTTTT
AAGTGTACAGTTATTCAATCAACGAATGGAATGCCTAAATTTAAAGACAAAACAGGCGGG
ACTTTAAGAAGGTTATTGATAGTACCGTTTAATGCCAATTTTAATGGCATTAAAGAGAAT
TTTAAAATCAAAGAAGACTATATAAAAAATCAGCAAGTGCTAGAGTATGTGCTTTATAAA
GCAATTAATTTAGATTTTGAAACCTTTGACATTCCTGATGCATCTAAAAAAATGCTTGAA
GTATTTAAAGAAGATAACGATCCAGTTTATGGGTTTAAAGTAAATATGTTTGATCAATGG
ACTATTAGAAAAGTGCCGAAATATATTGTATACGCATTTTATAAAGAATATTGTGATGAA
AATGGCTATAATGCATTGAGTTCAAACAAGTTTTATAAACAATTTGAACATTATTTAGAG
AATTATTGGAAAACTGATGCACAGCGAAGATATGACAATGAAGAACTTGCTAAGAGGATA
TACAACTTTAATGACAATAGAAATTACATTGAACCTATTGAAAGTGGAAAAAACTATAAA
TCGTATGAAAAGGTGAAGCTAAAAGCAATATAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS04365 [old locus tag: SAUSA300_0809 ]
- symbol: SAUSA300_RS04365
- description: primase
- length: 790
- theoretical pI: 6.08403
- theoretical MW: 92004.7
- GRAVY: -0.631646
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair phage/plasmid primase, P4 family, C-terminal domain (TIGR01613; HMM-score: 370.7)Mobile and extrachromosomal element functions Prophage functions phage/plasmid primase, P4 family, C-terminal domain (TIGR01613; HMM-score: 370.7)and 1 moreHipA N-terminal domain (TIGR03071; HMM-score: 14.1)
- TheSEED: see SAUSA300_0809
- PFAM: HTH (CL0123) Pox_D5; Poxvirus D5 protein-like (PF03288; HMM-score: 54.3)and 2 moreno clan defined D5_N; D5 N terminal like (PF08706; HMM-score: 39.9)HTH (CL0123) RFX_DNA_binding; RFX DNA-binding domain (PF02257; HMM-score: 16.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.008251
- TAT(Tat/SPI): 0.000748
- LIPO(Sec/SPII): 0.000885
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446907979 NCBI
- RefSeq: WP_000985235 NCBI
- UniProt: see SAUSA300_0809
⊟Protein sequence[edit | edit source]
- MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKVPYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKKGQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTVTGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSKQKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWESKRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEENWIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIKRVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPDYIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRKKAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVPVGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRRLLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVFKEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLENYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.