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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0814 [new locus tag: SAUSA300_RS04390 ]
  • pan locus tag?: SAUPAN002872000
  • symbol: SAUSA300_0814
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

abiD : abortive infection system protein AbiD [1]

• abiD results in a > 4-log reduction in efficiency-of-plating of Siphovirus Sebago

• abiD results in approximately a 1 - 2 log reduction in efficiency-of-plating of Siphovirus Φ12

• abiD has no effect on Siphovirus Φ11, Φ29, Φ52a, Φ55, Φ80α, Φ85, Φ6480, Φ7093, Φ7094, Φ7903, Φ8490, ΦNM1, ΦNM2, ΦNM4, kayvirus Sb1, ISP, 812, K, Maine, silviavirus Romulus or Remus

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0814 [new locus tag: SAUSA300_RS04390 ]
  • symbol: SAUSA300_0814
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 893372..894340
  • length: 969
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGCCATTTGAAAGTCATAATAAACAATTGAAAATTCTAAGAAGAAGAGGAATGGAA
    GTACCGAGTAGTGCTAAAAGAGATTTAGAAAATGAAAATTATTATAATATCATAAATGGT
    TATAAAGATTTATTTTTAGAACTAGATGTTAATGGTAATTTTTTGGTTCCTGATAAATAT
    AAGCAAGGTACTCATTTTAAAGAAGTCTTTTCTTTATACAAACTAGATAGAAAATTTAGG
    AATGTTTTATTAGAGTATTTGTTAGTATTTGAAACTCATATTAAATCAAGAATTTCATAT
    TATTTTAGCGAAAAATATAGAGAACCACATTCATATTTATACTTTAAAAATTATTCATCT
    GACACAAGTAAGACAGATAGCATCGTGAAAATGGTTGCTACATTTAGCTCGGTTATGAGT
    AATAGAAAAAATAAACCATTAAAACATTATATTAATACTCATAATGGAGTGCCACTATGG
    ATATTGGTGAATTATTTAACTTTAGGTAATGTTTCAAAAATGTATTCCAATTTGGATGAT
    GATCTTCGATTGGAAGTTGCTAAAGACTATAAAAGGAAATTGGAAAGAGATTATAAAACA
    CGTGTTCAAATAACTCCATCAGATGTAGACAGTATACTACAACAAGCACATATGTTTCGT
    AACGTGTGTGCGCATGAAGAAAGATTGTATGATTATAAAATAGACAGGGCTAAAAGTAGA
    GCTAATATATTCGCCAATTATAACAAAATATACGATAAAGAATACGTTCCTACAATGAAT
    GGTAGTTATGTATTCGATTTGTTGATTTCACTATGTCTATTTTTGAATAAACATGATTAC
    ATAAAATTGATGAAAAATATGGATAAACTAATAAGTAATTATTCACATTCTTTCTATACA
    ATTACTATAGATGACCTATATACAAAAATGAATTTTCCAGATCAAACAAAAATACTGGAT
    ATGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0814 [new locus tag: SAUSA300_RS04390 ]
  • symbol: SAUSA300_0814
  • description: hypothetical protein
  • length: 322
  • theoretical pI: 9.5862
  • theoretical MW: 38520
  • GRAVY: -0.57764

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Hypothetical SAV0801 homolog in superantigen-encoding pathogenicity islands SaPI
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Hypothetical SAV0801 homolog in superantigen-encoding pathogenicity islands SaPI
  • PFAM:
    no clan defined Abi_2; Abi-like protein (PF07751; HMM-score: 139.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.5422
    • Cytoplasmic Membrane Score: 0.058
    • Cell wall & surface Score: 0.0032
    • Extracellular Score: 0.3966
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006492
    • TAT(Tat/SPI): 0.000673
    • LIPO(Sec/SPII): 0.001075
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDLFLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYFSEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLWILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHMFRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLNKHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ J Anba, E Bidnenko, A Hillier, D Ehrlich, M C Chopin
    Characterization of the lactococcal abiD1 gene coding for phage abortive infection.
    J Bacteriol: 1995, 177(13);3818-23
    [PubMed:7601848] [WorldCat.org] [DOI] (P p)    Marie-Christine Chopin, Alain Chopin, Elena Bidnenko
    Phage abortive infection in lactococci: variations on a theme.
    Curr Opin Microbiol: 2005, 8(4);473-9
    [PubMed:15979388] [WorldCat.org] [DOI] (P p)    Nitzan Aframian, Avigdor Eldar
    Abortive infection antiphage defense systems: separating mechanism and phenotype.
    Trends Microbiol: 2023, 31(10);1003-1012
    [PubMed:37268559] [WorldCat.org] [DOI] (I p)    Sarah M Voss, Katharine C King, Devin J Hunt, Adam A Wilson, Bruria Samuel, Orazio R Bagno, Peter F W Sparklin, Bridget Cassata, Joshua W Modell
    Engineered phages evade the complete defense repertoire of highly phage-resistant MRSA clinical isolates.
    bioRxiv: 2026;
    [PubMed:41929026] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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