PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0944 [new locus tag: SAUSA300_RS05075 ]
• pan locus tag^?: SAUPAN003242000
• symbol: menA
• pan gene symbol^?: menA
• synonym:
• product: 1,4-dihydroxy-2-naphthoate octaprenyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0944 [new locus tag: SAUSA300_RS05075 ]
• symbol: menA
• product: 1,4-dihydroxy-2-naphthoate octaprenyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1031928..1032866
• length: 939
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913662 NCBI
• RefSeq: YP_493642 NCBI
• BioCyc: see SAUSA300_RS05075
• MicrobesOnline: 1292459 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  901
  
  ATGAGTAATCAATATCAGCAATATTCTACAGTTAAGAAATATTGGCATTTAATGCGTCCT
  CATACATTAACTGCTTCCGTAGTACCCGTTTTAGTTGGTACAGCAGCATCTAAAATATAT
  TTTCTTGGTAGCGAAGATCATATTAAAATCAGCCTATTCATTGCCATGTTACTAGCATGC
  TTACTTATTCAAGCAGCAACTAATATGTTTAATGAATACTATGATTATAAAAAAGGCCTT
  GATGATCATGAATCTGTAGGCATTGGTGGTGCCATTGTTCGCAACGGTATGAGCCCAGAG
  CTTGTGCTACGATTAGCCATTGCATTTTACATCTTAGCAGCAATATTAGGTTTGTTTTTA
  GCTGCTAACTCTTCATTTTGGTTATTACCAGTTGGATTAGTATGTATGGCTGTTGGTTAC
  CTATATACAGGTGGCCCTTTCCCTATTTCATGGACGCCTTTCGGTGAATTATTCTCAGGC
  GTATTTATGGGTATGTTTATTATCGTTATTGCATTCTTTATTCAAACTGGCAATATTCAA
  AGTTATGTAATTTGGTTAAGTGTACCTATAGTAATCACTATCGGTTTAATTAATATGGCT
  AACAATATTCGCGACCGTGTCAAAGATAAAGCAAGTGGTCGCAAAACTTTACCCATTCTA
  TTAGGTAAAAATGCTTCTTTAACATTTATGGCAATCATGTACTTTATCGCTTATGCCTTT
  ATTGTACTTACGATCATTATTAAACCTGGTGGCTCATTATTTTACTTACTTGCGTTGTTA
  TCATTCCCAATGCCTGTTAAAGTTATCAGACGTTTCAAGAAGAATGATACACCGCCTACA
  ATGATGCCAGCAATGGCTGCTGCTGGTAAAACAAATACATTTTTCGGTTTATTATATGCA
  TTAGGTATTTATATTAGTGCATTATTTGCAGGCATTTAA
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  939
  

⊟Protein

⊟General

• locus tag: SAUSA300_0944 [new locus tag: SAUSA300_RS05075 ]
• symbol: MenA
• description: 1,4-dihydroxy-2-naphthoate octaprenyltransferase
• length: 312
• theoretical pI: 9.91895
• theoretical MW: 34400
• GRAVY: 0.733654

⊟Function

• reaction:
  EC 2.5.1.74?  ExPASy
  1,4-dihydroxy-2-naphthoate polyprenyltransferase An all-trans-polyprenyl diphosphate + 1,4-dihydroxy-2-naphthoate = a demethylmenaquinol + diphosphate + CO₂
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 1,4-dihydroxy-2-naphthoate octaprenyltransferase (TIGR00751; EC 2.5.1.74; HMM-score: 99.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 1,4-dihydroxy-2-naphthoate phytyltransferase (TIGR02235; EC 2.5.1.-; HMM-score: 98.1)
  and 2 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthase ChlG (TIGR02056; EC 2.5.1.62; HMM-score: 23.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone putative 4-hydroxybenzoate polyprenyltransferase (TIGR01475; EC 2.5.1.-; HMM-score: 21.5)
• TheSEED  :
  • 1,4-dihydroxy-2-naphthoate polyprenyltransferase (EC 2.5.1.74)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Quinone cofactors Menaquinone and Phylloquinone Biosynthesis  1,4-dihydroxy-2-naphthoate polyprenyltransferase (EC 2.5.1.74)
  and 1 more
  Cofactors, Vitamins, Prosthetic Groups, Pigments Quinone cofactors Menaquinone biosynthesis from chorismate via 1,4-dihydroxy-2-naphthoate  1,4-dihydroxy-2-naphthoate polyprenyltransferase (EC 2.5.1.74)
• PFAM:
  Terp_synthase (CL0613) UbiA; UbiA prenyltransferase family (PF01040; HMM-score: 126.4)
  and 2 more
  no clan defined UPF0767; UPF0767 family (PF15990; HMM-score: 10.6)
  DAGK_prokar; Prokaryotic diacylglycerol kinase (PF01219; HMM-score: 7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 9
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010098
  • TAT(Tat/SPI): 0.000713
  • LIPO(Sec/SPII): 0.016984
• predicted transmembrane helices (TMHMM): 9

⊟Accession numbers

• GI: 87160260 NCBI
• RefSeq: YP_493642 NCBI
• UniProt: A0A0H2XEM6 UniProt

⊟Protein sequence

• MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGSEDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELVLRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSGVFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLPILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKNDTPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications