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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0977 [new locus tag: SAUSA300_RS05250 ]
  • pan locus tag?: SAUPAN003289000
  • symbol: SAUSA300_0977
  • pan gene symbol?: thiX
  • synonym:
  • product: cobalt transport family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0977 [new locus tag: SAUSA300_RS05250 ]
  • symbol: SAUSA300_0977
  • product: cobalt transport family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1070946..1071752
  • length: 807
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTATGAGCAATGGAAAGTTCGGCAATCATTTGTTGATGATGTCAACATTATAACGAAA
    CTATTTCTTGGAATTATACTATTTTTCTTTATTATATTTGTGCATAATTTCGATTTTATG
    CTTTATATCACTATCTTAATGTTAGTTTTCTTATTGATGTTTAATGGTACTCAGTTTAAA
    ATTACAGCAATTTTTATAACTATAAGTATTGGCTTTGCATTACTCTCTTCCCTGTTCATG
    ATTTTATACGGGAATGGTAGCCATACAATTTTGAAATTCGGATTTATCCATATTACAACA
    GAAAGTTTAGTTCGTGGTTTACATGTATCATTAAGAACCATTGCTATTTCATTTTTCGGT
    ATCTTAATCGCACTTACATCTCAGGTTGTCATGATATTTTATAGTTTAATGCAACATTTA
    AAGGTTAAATCAAAAGTTGCTTATGCATTCATGGCTGCCATTCGTATGATACCTTTAATG
    ATTAGTTCTTTAATACAGCTTCGCAGATCTTTAAAAATGCGCTATCAGATGATAGACGCG
    GCAAATTATAGAGGGTTAAAGCGATTTAAGCATTTAATTATACCTCTATTAAGCCAAAAT
    ATTAGAAAGGCACATCAATTATCAGTTGCGATGGAGTCGAAAGGATTTAGAGATGGTCCT
    CGTACTTACTATTACAACGTACCTTTCTCATATAAAGATATAATCTTTATTATAGTTTTA
    ATTGCCATTATTACTATGGCATATTATTGTTCAATCACCTTACCCATCACGGGCATAGAT
    GATGTTCGTCTTGGTCGTGTAGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    807

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0977 [new locus tag: SAUSA300_RS05250 ]
  • symbol: SAUSA300_0977
  • description: cobalt transport family protein
  • length: 268
  • theoretical pI: 10.5187
  • theoretical MW: 30953.2
  • GRAVY: 0.758582

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ECF transporter T component CbiQ (TIGR02454; HMM-score: 53.8)
  • TheSEED  :
    • Thiamin-regulated hydroxymethylpyrimidine ECF transporter, transmembrane component of energizing module YkoC
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Transmembrane component YkoC of energizing module of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine
    and 1 more
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Transmembrane component YkoC of energizing module of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine
  • PFAM:
    no clan defined CbiQ; Cobalt transport protein (PF02361; HMM-score: 82.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000557
    • TAT(Tat/SPI): 0.000079
    • LIPO(Sec/SPII): 0.004336
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYITILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTESLVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLMISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRDGPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: THI-box (transcription termination) regulon
    THI-box(RNA)important in Thiamine biosynthesis; transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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