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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0977 [new locus tag: SAUSA300_RS05250 ]
- pan locus tag?: SAUPAN003289000
- symbol: SAUSA300_0977
- pan gene symbol?: thiX
- synonym:
- product: cobalt transport family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0977 [new locus tag: SAUSA300_RS05250 ]
- symbol: SAUSA300_0977
- product: cobalt transport family protein
- replicon: chromosome
- strand: -
- coordinates: 1070946..1071752
- length: 807
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3915197 NCBI
- RefSeq: YP_493675 NCBI
- BioCyc: see SAUSA300_RS05250
- MicrobesOnline: 1292492 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGTATGAGCAATGGAAAGTTCGGCAATCATTTGTTGATGATGTCAACATTATAACGAAA
CTATTTCTTGGAATTATACTATTTTTCTTTATTATATTTGTGCATAATTTCGATTTTATG
CTTTATATCACTATCTTAATGTTAGTTTTCTTATTGATGTTTAATGGTACTCAGTTTAAA
ATTACAGCAATTTTTATAACTATAAGTATTGGCTTTGCATTACTCTCTTCCCTGTTCATG
ATTTTATACGGGAATGGTAGCCATACAATTTTGAAATTCGGATTTATCCATATTACAACA
GAAAGTTTAGTTCGTGGTTTACATGTATCATTAAGAACCATTGCTATTTCATTTTTCGGT
ATCTTAATCGCACTTACATCTCAGGTTGTCATGATATTTTATAGTTTAATGCAACATTTA
AAGGTTAAATCAAAAGTTGCTTATGCATTCATGGCTGCCATTCGTATGATACCTTTAATG
ATTAGTTCTTTAATACAGCTTCGCAGATCTTTAAAAATGCGCTATCAGATGATAGACGCG
GCAAATTATAGAGGGTTAAAGCGATTTAAGCATTTAATTATACCTCTATTAAGCCAAAAT
ATTAGAAAGGCACATCAATTATCAGTTGCGATGGAGTCGAAAGGATTTAGAGATGGTCCT
CGTACTTACTATTACAACGTACCTTTCTCATATAAAGATATAATCTTTATTATAGTTTTA
ATTGCCATTATTACTATGGCATATTATTGTTCAATCACCTTACCCATCACGGGCATAGAT
GATGTTCGTCTTGGTCGTGTAGGTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0977 [new locus tag: SAUSA300_RS05250 ]
- symbol: SAUSA300_0977
- description: cobalt transport family protein
- length: 268
- theoretical pI: 10.5187
- theoretical MW: 30953.2
- GRAVY: 0.758582
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds cobalt ECF transporter T component CbiQ (TIGR02454; HMM-score: 53.8)
- TheSEED :
- Thiamin-regulated hydroxymethylpyrimidine ECF transporter, transmembrane component of energizing module YkoC
Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis Transmembrane component YkoC of energizing module of thiamin-regulated ECF transporter for HydroxyMethylPyrimidineand 1 more - PFAM: no clan defined CbiQ; Cobalt transport protein (PF02361; HMM-score: 82.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 5
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.000557
- TAT(Tat/SPI): 0.000079
- LIPO(Sec/SPII): 0.004336
- predicted transmembrane helices (TMHMM): 6
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYITILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTESLVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLMISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRDGPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: THI-box (transcription termination) regulon
THI-box (RNA) important in Thiamine biosynthesis; transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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