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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1024 [new locus tag: SAUSA300_RS05510 ]
  • pan locus tag?: SAUPAN003352000
  • symbol: coaD
  • pan gene symbol?: coaD
  • synonym:
  • product: phosphopantetheine adenylyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1024 [new locus tag: SAUSA300_RS05510 ]
  • symbol: coaD
  • product: phosphopantetheine adenylyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1119577..1120059
  • length: 483
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGAACATACAATAGCGGTCATTCCGGGTAGTTTTGACCCCATTACTTATGGTCATTTA
    GACATTATTGAGAGAAGTACAGATAGATTTGATGAAATTCATGTCTGTGTTCTTAAAAAT
    AGTAAAAAAGAAGGTACGTTTAGTTTAGAAGAGCGTATGGATTTAATTGAACAATCTGTT
    AAACATTTACCTAATGTCAAGGTTCATCAATTTAGTGGTTTACTAGTCGATTATTGTGAA
    CAAGTAGGAGCTAAAACAATCATACGTGGTTTAAGAGCAGTCAGTGATTTTGAATATGAA
    TTACGCTTAACTTCAATGAATAAAAAGTTGAACAATGAAATTGAAACGTTATATATGATG
    TCTAGTACTAATTATTCATTTATAAGTTCAAGTATTGTTAAAGAAGTTGCAGCTTATCGA
    GCAGATATTTCTGAATTCGTTCCACCTTATGTTGAAAAGGCATTGAAGAAGAAATTTAAG
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    483

⊟Protein[edit | edit source]

Protein Data Bank: 3F3M

⊟General[edit | edit source]

  • locus tag: SAUSA300_1024 [new locus tag: SAUSA300_RS05510 ]
  • symbol: CoaD
  • description: phosphopantetheine adenylyltransferase
  • length: 160
  • theoretical pI: 6.67902
  • theoretical MW: 18371
  • GRAVY: -0.245

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.3?  ExPASy
    Pantetheine-phosphate adenylyltransferase ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A pantetheine-phosphate adenylyltransferase (TIGR01510; EC 2.7.7.3; HMM-score: 207.5)
    and 6 more
    cytidyltransferase-like domain (TIGR00125; HMM-score: 57)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinate (nicotinamide) nucleotide adenylyltransferase (TIGR00482; EC 2.7.7.18; HMM-score: 39.9)
    Genetic information processing Protein fate Protein modification and repair [citrate (pro-3S)-lyase] ligase (TIGR00124; EC 6.2.1.22; HMM-score: 34.1)
    Metabolism Energy metabolism Fermentation [citrate (pro-3S)-lyase] ligase (TIGR00124; EC 6.2.1.22; HMM-score: 34.1)
    nicotinamide-nucleotide adenylyltransferase (TIGR01526; EC 2.7.7.1; HMM-score: 25.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinamide-nucleotide adenylyltransferase (TIGR01527; EC 2.7.7.1; HMM-score: 23)
  • TheSEED  :
    • Phosphopantetheine adenylyltransferase (EC 2.7.7.3)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Phosphopantetheine adenylyltransferase (EC 2.7.7.3)
  • PFAM:
    HUP (CL0039) CTP_transf_like; Cytidylyltransferase-like (PF01467; HMM-score: 67.6)
    and 1 more
    Citrate_ly_lig; Citrate lyase ligase C-terminal domain (PF08218; HMM-score: 39.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001605
    • TAT(Tat/SPI): 0.000102
    • LIPO(Sec/SPII): 0.000239
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKEGTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELRLTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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