PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1104 [new locus tag: SAUSA300_RS05980 ]
• pan locus tag^?: SAUPAN003493000
• symbol: coaBC
• pan gene symbol^?: coaBC
• synonym:
• product: phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1104 [new locus tag: SAUSA300_RS05980 ]
• symbol: coaBC
• product: phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase
• replicon: chromosome
• strand: +
• coordinates: 1207854..1209053
• length: 1200
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914611 NCBI
• RefSeq: YP_493801 NCBI
• BioCyc: see SAUSA300_RS05980
• MicrobesOnline: 1292619 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAGAAAATATTATTAGCCGTTACAGGTGGCATTGCGGCATATAAAGCAATTGATTTG
  ACAAGTAAGTTAACACAATCTGGGTATGAAGTTCGCGTTATGTTAACGAATCACGCACAA
  AAATTTGTGACACCATTAGCATTTCAAGCAATAAGTCGAAATGCTGTTTATACAGATACT
  TTTATAGAAGAAAATCCTTCAGAAATACAGCATATTGCATTAGGTGATTGGGCAGATGCA
  ATCATTGTTGCACCTGCAACGGCAAATACAATTGCAAAATTGAGTGTAGGTATTGCTGAT
  GATTTGGTGACATCAACGTTGCTAGCAACAGAGACACCGAAATTTATTGCGCCTGCTATG
  AATGTGCATATGTATGAAAATAAACGTACGCAGCAAAATATTAATATTTTAAAAGAAGAT
  GGGTATCATTTTATCGAACCAGGAAGCGGATTTCTAGCATGTGGTTATGTTGCTAAAGGA
  CGTATGGAAGAACCGCTTCAAATCGTTTCTGTAATTGATGCTCATTTTCAAAATAGTAAT
  CGTTTAGCTAATAGTTCATTTCAAGATAAACGCGCATTGGTTACAGCAGGACCAACTATT
  GAAGTTATCGATCCAGTCAGATTTGTATCCAATCGTTCTTCTGGAAAAATGGGCTATGCA
  ATAGCTGAAGCATTGCGAAATCGAGGAGCTATCGTGACGTTAGTTGCTGGTCCTACAACA
  CTAGAGGATCCAAAAGATATTGAAGTTATTCATGTTCAAAGTGCTGAAGAAATGTTTGAA
  CAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGAC
  TATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGGTGATTTGTCAGTA
  TCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTAT
  TTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTACGC
  AAGAAAAATGCAGATGTGATTATTTCAAATAATGTTGGGGATATGTCTATCGGATTTAGT
  TCTGATGATAATGAATTGACAATGCATTTTAAAAATAATGAAAAAGTAAATATCAAGAAA
  GGAAAAAAAGTAGTATTAGCTGCACAAATTTTAGATGAACTAGAAACTAGGTGGCAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1104 [new locus tag: SAUSA300_RS05980 ]
• symbol: CoaBC
• description: phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase
• length: 399
• theoretical pI: 5.87138
• theoretical MW: 44140
• GRAVY: -0.211278

⊟Function

• reaction:
  EC 6.3.2.5?  ExPASy
  Phosphopantothenate--cysteine ligase (CTP) CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine
  EC 4.1.1.36?  ExPASy
  Phosphopantothenoylcysteine decarboxylase N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO₂
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase (TIGR00521; EC 4.1.1.36,6.3.2.5; HMM-score: 478.1)
  and 6 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase (TIGR02113; EC 4.1.1.36; HMM-score: 208.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenate--cysteine ligase (TIGR02114; EC 6.3.2.5; HMM-score: 72.2)
  Metabolism Energy metabolism Electron transport archaeoflavoprotein AfpA (TIGR02699; HMM-score: 32.3)
  Unknown function General archaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 31.4)
  Unknown function Enzymes of unknown specificity polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases (TIGR00421; EC 4.1.1.-; HMM-score: 27.7)
  Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, B subunit (TIGR02852; HMM-score: 16.4)
• TheSEED  :
  • Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
  • Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
  and 1 more
  Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)
• PFAM:
  NADP_Rossmann (CL0063) DFP; DNA / pantothenate metabolism flavoprotein (PF04127; HMM-score: 233.3)
  and 4 more
  no clan defined Flavoprotein; Flavoprotein (PF02441; HMM-score: 161.6)
  NADP_Rossmann (CL0063) YjeF_N; YjeF-related protein N-terminus (PF03853; HMM-score: 17.6)
  adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 15.9)
  SPFH (CL0433) Band_7; SPFH domain / Band 7 family (PF01145; HMM-score: 14.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: FMN
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.128674
  • TAT(Tat/SPI): 0.000733
  • LIPO(Sec/SPII): 0.04171
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160791 NCBI
• RefSeq: YP_493801 NCBI
• UniProt: A0A0H2XFW7 UniProt

⊟Protein sequence

• MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVTPLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDLVTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKGRMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMGYAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAAAVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENYAQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILDELETRWQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: coaBC > priA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications