PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1111 [new locus tag: SAUSA300_RS06015 ]
• pan locus tag^?: SAUPAN003501000
• symbol: SAUSA300_1111
• pan gene symbol^?: rlmN
• synonym:
• product: ribosomal RNA large subunit methyltransferase N

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1111 [new locus tag: SAUSA300_RS06015 ]
• symbol: SAUSA300_1111
• product: ribosomal RNA large subunit methyltransferase N
• replicon: chromosome
• strand: +
• coordinates: 1216308..1217402
• length: 1095
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915122 NCBI
• RefSeq: YP_493808 NCBI
• BioCyc: see SAUSA300_RS06015
• MicrobesOnline: 1292626 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGATAACTGCTGAAAAGAAAAAGAAGAATAAATTTCTTCCAAATTTTGACAAGCAATCA
  ATATATTCATTGCGATTTGACGAAATGCAAAACTGGCTCGTTGAACAAGGGCAACAAAAA
  TTTCGAGCGAAACAGATTTTTGAATGGTTATATCAAAAAAGAGTAGATTCGATTGATGAA
  ATGACGAACTTATCGAAAGACTTACGACAGCTTTTAAAAGATAACTTTACTGTTACAACT
  TTAACAACTGTAGTAAAACAAGAAAGTAAAGACGGTACAATTAAATTCTTATTTGAATTA
  CAAGATGGCTATACAATTGAAACTGTTTTAATGAGACATGATTATGGAAATTCAGTATGT
  GTAACGACACAAGTAGGTTGTCGCATCGGATGTACGTTTTGTGCTTCTACACTTGGCGGC
  TTAAAAAGAAACCTTGAAGCTGGCGAAATTGTTTCACAAGTTTTAACAGTTCAAAAAGCC
  CTTGATGCTACAGAAGAGCGCGTATCTCAAATTGTCATAATGGGTATCGGTGAACCATTT
  GAAAATTATGATGAAATGATGGACTTTTTAAGAATCGTCAATGATGATAACAGTTTAAAT
  ATTGGTGCACGTCACATTACAGTATCAACATCAGGTATCATTCCTAGAATATATGACTTT
  GCGGATGAAGATATTCAAATTAATTTTGCTGTAAGCTTACACGCCGCAAAAGATGAAGTG
  CGATCACGCTTGATGCCAATTAACCGTGCATATAATGTTGAGAAGTTAATCGAAGCAATT
  CAATATTATCAAGAAAAAACAAATCGTCGTGTTACTTTTGAATATGGTCTGTTTGGTGGT
  GTGAATGACCAACTAGAACATGCAAGAGAATTAGCACATTTAATAAAAGGCTTAAACTGC
  CATGTTAACTTAATTCCTGTCAACCATGTTCCAGAAAGAAATTATGTGAAAACGGCTAAA
  AATGATATCTTTAAATTTGAAAAAGAATTAAAGAGACTAGGAATTAATGCCACAATACGT
  CGTGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAA
  GTAGAAACGAGGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1111 [new locus tag: SAUSA300_RS06015 ]
• symbol: SAUSA300_1111
• description: ribosomal RNA large subunit methyltransferase N
• length: 364
• theoretical pI: 7.15306
• theoretical MW: 41904.6
• GRAVY: -0.428022

⊟Function

• reaction:
  EC 2.1.1.192?  ExPASy
  23S rRNA (adenine²⁵⁰³-C₂)-methyltransferase 2 S-adenosyl-L-methionine + adenine²⁵⁰³ in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine²⁵⁰³ in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin 2 S-adenosyl-L-methionine + adenine³⁷ in tRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine³⁷ in tRNA + 2 oxidized [2Fe-2S] ferredoxin
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (adenine(2503)-C(2))-methyltransferase (TIGR00048; EC 2.1.1.192; HMM-score: 510.6)
  and 9 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (adenine(2503)-C(8))-methyltransferase Cfr (TIGR04432; EC 2.1.1.224; HMM-score: 251.5)
  hopanoid biosynthesis associated radical SAM protein HpnH (TIGR03470; HMM-score: 22.6)
  Genetic information processing Protein fate Protein modification and repair anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 19.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 19.3)
  radical SAM enzyme, TIGR04100 family (TIGR04100; HMM-score: 19.1)
  Cellular processes Cellular processes Adaptations to atypical conditions KamA family protein (TIGR00238; HMM-score: 17.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 17.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02826; EC 1.97.1.-; HMM-score: 15.1)
  Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium transporter (TIGR00400; HMM-score: 10.5)
• TheSEED  :
  • 23S rRNA (adenine(2503)-C(2))-methyltransferase
  • tRNA (adenine(37)-C(2))-methyltransferase (EC 2.1.1.192)
  Conserved gene cluster associated with Met-tRNA formyltransferase  Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)
• PFAM:
  TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 69.1)
  and 4 more
  4Fe-4S (CL0344) Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 29.7)
  Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 19.4)
  no clan defined Pox_RNA_Pol_22; Poxvirus RNA polymerase 22 kDa subunit (PF05273; HMM-score: 13.4)
  Peptidase_CA (CL0125) Peptidase_C23; Carlavirus endopeptidase (PF05379; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: [4Fe-4S] cluster
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001615
  • TAT(Tat/SPI): 0.000149
  • LIPO(Sec/SPII): 0.000265
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160347 NCBI
• RefSeq: YP_493808 NCBI
• UniProt: Q2FHM0 UniProt

⊟Protein sequence

• MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQVETR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: def > fmt > sun > SAUSA300_1111 > SAUSA300_1112 > pknB

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications