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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1176 [new locus tag: SAUSA300_RS06360 ]
  • pan locus tag?: SAUPAN003585000
  • symbol: pgsA
  • pan gene symbol?: pgsA
  • synonym:
  • product: CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1176 [new locus tag: SAUSA300_RS06360 ]
  • symbol: pgsA
  • product: CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1294072..1294650
  • length: 579
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAATATTCCGAACCAGATTACGGTTTTTAGAGTAGTGTTAATACCAGTTTTTATATTG
    TTTGCGTTAGTTGATTTTGGATTTGGCAATGTGTCATTTCTAGGAGGATATGAAATAAGA
    ATTGAGTTATTAATCAGTGGTTTTATTTTTATATTGGCTTCCCTTAGCGATTTTGTTGAT
    GGTTATTTAGCTAGAAAATGGAATTTAGTTACAAATATGGGGAAATTTTTGGATCCATTA
    GCGGATAAATTATTAGTTGCAAGTGCTTTAATTGTACTTGTGCAACTAGGACTAACAAAT
    TCTGTAGTAGCAATCATTATTATTGCCAGAGAATTTGCCGTAACTGGTTTACGTTTACTA
    CAAATTGAACAAGGATTCGTAAGTGCAGCTGGTCAATTAGGTAAAATTAAAACAGCAGTT
    ACTATGGTAGCAATTACTTGGTTGTTATTAGGTGATCCATTGGCAACATTGATTGGTTTG
    TCATTAGGACAAATTTTATTATACATTGGCGTTATTTTTACTATCTTATCTGGTATTGAA
    TACTTTTATAAAGGTAGAGATGTTTTTAAACAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    579

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1176 [new locus tag: SAUSA300_RS06360 ]
  • symbol: PgsA
  • description: CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
  • length: 192
  • theoretical pI: 9.39765
  • theoretical MW: 21014.1
  • GRAVY: 1.02448

Function[edit | edit source]

  • reaction:
    EC 2.7.8.5?  ExPASy
    CDP-diacylglycerol--glycerol-3-phosphate 1-phosphatidyltransferase CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (TIGR00560; EC 2.7.8.5; HMM-score: 191.2)
    and 1 more
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis CDP-diacylglycerol-serine O-phosphatidyltransferase (TIGR00473; EC 2.7.8.8; HMM-score: 33.4)
  • TheSEED:  
    Clustering-based subsystems Two related proteases CBSS-257314.1.peg.676  CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5)
    and 2 more
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5)
    Miscellaneous Plant-Prokaryote comparative genomics Competence or DNA damage-inducible protein CinA and related protein families  CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5)
  • PFAM:
    no clan defined CDP-OH_P_transf; CDP-alcohol phosphatidyltransferase (PF01066; HMM-score: 60.1)
    and 1 more
    Holin-II (CL0563) Phage_holin_2_3; Bacteriophage holin family HP1 (PF16080; HMM-score: 13.4)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019369
    • TAT(Tat/SPI): 0.000171
    • LIPO(Sec/SPII): 0.003491
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELLISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSVVAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGLSLGQILLYIGVIFTILSGIEYFYKGRDVFKQK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]