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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1283 [new locus tag: SAUSA300_RS06965 ]
  • pan locus tag?: SAUPAN003798000
  • symbol: pstS
  • pan gene symbol?: pstS
  • synonym:
  • product: phosphate ABC transporter phosphate-binding protein PstS

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1283 [new locus tag: SAUSA300_RS06965 ]
  • symbol: pstS
  • product: phosphate ABC transporter phosphate-binding protein PstS
  • replicon: chromosome
  • strand: -
  • coordinates: 1411339..1412322
  • length: 984
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAAATGGCAATTTGTTGGTACTACAGCTTTAGGTGCAACACTATTATTAGGTGCT
    TGTGGTGGCGGTAATGGTGGCAGTGGTAATAGTGATTTAAAAGGGGAAGCTAAAGGTGAT
    GGCTCATCAACAGTAGCACCAATTGTGGAGAAATTAAATGAAAAATGGGCTCAAGATCAC
    TCGGATGCTAAAATCTCAGCAGGACAAGCTGGTACAGGTGCTGGTTTCCAAAAATTCATT
    GCAGGAGATATCGACTTCGCTGATGCTTCTAGACCAATTAAAGATGAAGAGAAGCAAAAA
    TTACAAGATAAGAATATCAAATACAAAGAATTCAAAATTGCGCAAGATGGTGTAACGGTT
    GCTGTAAATAAAGAAAATGATTTTGTAGATGAATTAGACAAACAGCAATTAAAAGCAATT
    TATTCTGGAAAAGCTAAAACATGGAAAGATGTTAATAGTAAATGGCCAGATAAAAAAATA
    AATGCTGTATCACCAAACTCAAGTCATGGTACTTATGACTTCTTTGAAAATGAAGTAATG
    AATAAAGAAGATATTAAAGCAGAAAAAAATGCTGATACAAATGCTATCGTTTCTTCTGTA
    ACGAAAAACAAAGAGGGAATCGGATACTTTGGATATAACTTCTACGTACAAAATAAAGAT
    AAATTAAAAGAAGTTAAAATCAAAGATGAAAATGGTAAAGCAACAGAGCCTACGAAAAAA
    ACAATTCAAGATAACTCTTATGCATTAAGTAGACCATTATTCATTTATGTAAATGAAAAA
    GCATTGAAAGATAATAAAGTAATGTCAGAATTTATCAAATTCGTCTTAGAAGATAAAGGT
    AAAGCAGCTGAAGAAGCTGGATATGTAGCAGCACCAGAGAAAACATACAAATCACAATTA
    GATGATTTAAAAGCATTTATTGATAAAAATCAAAAATCAGACGACAAGAAATCTGATGAT
    AAAAAGTCTGAAGACAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1283 [new locus tag: SAUSA300_RS06965 ]
  • symbol: PstS
  • description: phosphate ABC transporter phosphate-binding protein PstS
  • length: 327
  • theoretical pI: 8.71151
  • theoretical MW: 36117.2
  • GRAVY: -0.870031

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphate binding protein (TIGR02136; HMM-score: 344.1)
    and 2 more
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, phosphate-binding protein PstS (TIGR00975; HMM-score: 65)
    phosphate ABC transporter substrate-binding protein (TIGR04505; HMM-score: 50.1)
  • TheSEED  :
    • Phosphate ABC transporter, substrate-binding protein PstS
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory High affinity phosphate transporter and control of PHO regulon  Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)
    and 1 more
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)
  • PFAM:
    PBP (CL0177) PBP_like_2; PBP superfamily domain (PF12849; HMM-score: 137.8)
    and 1 more
    PBP_like; PBP superfamily domain (PF12727; HMM-score: 60.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: LLLGACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.000881
    • TAT(Tat/SPI): 0.0001
    • LIPO(Sec/SPII): 0.998765
    • Cleavage Site: CS pos: 20-21. LGA-CG. Pr: 0.9991
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSSTVAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQDKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKINAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQNKDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVLEDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]