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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1292 [new locus tag: SAUSA300_RS07020 ]
  • pan locus tag?: SAUPAN003812000
  • symbol: alr2
  • pan gene symbol?: alr2
  • synonym:
  • product: alanine racemase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1292 [new locus tag: SAUSA300_RS07020 ]
  • symbol: alr2
  • product: alanine racemase
  • replicon: chromosome
  • strand: +
  • coordinates: 1422715..1423800
  • length: 1086
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGACAGCAACATGGTCTGTAAATAAGAAAATATTTTTACAAAATGCAATCACAGTCAAA
    AACAATCAGCCATTAATGGCAGTTGTTAAAAATAATGCATATCACTATGACCTAGAATTT
    GCTGTAACTCAGTTTATCCATGCAGGTATAGATACATTTAGCACAACATCACTACGAGAA
    GCAATTCAAATTAGACAACTTGCTCCAGATGCAACAATCTTTTTAATGAATGCAGTTTAC
    GAGTTTGATTTAGTCCGTGAACATCAAATACATATGACTTTGCCGTCGTTGACATATTAC
    TATAACCATAAAAATGATTTAGCTGGTATTCATGTTCACTTAGAATTTGAAAATTTATTA
    CATCGGTCTGGATTTAAAGATTTAAACGAAATTAAAGAAGTATTGAAAGATCACCATCAT
    AATCAAAATGCAAAAATGATTATTAGTGGTTTATGGACCCATTTTGGATATGCTGATGAA
    TTCGATGTGTCAGATTATAATGTTGAACGTTCACAATGGATGGAAATTGTTGAAGCACTT
    TTATCTGAAGGTTATCAGTTCGACCTAATCCATGCTCAAAATAGTGCGAGTTTTTATCGG
    GAAGGACAAATATTACTACCCCACCATACACATGCGCGTGTAGGTATTGCGTTATACGGT
    TCAAGACCATATAGTTCACTGAATCAACATGATATAGTTCAGTCATTAACTTTAAAAGCA
    CATGTTATTCAAGTGCGCGAAGTACAAGCTGGTGATTATTGCGGTTATAGCTTTGCCTTT
    GAAGTGACTAAAAACAATACAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATT
    TTAAGAACTCGTGCTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCA
    TTAATGATGAGCCATATGTTTGTTGAAGTAGATGGCAATGTACATGCACAAGATGAAGTT
    ATTCTTTATAATAATGATATCCGCATCGATGAATATACCTTTAAAGGTGTTGGTGCAAAT
    TCTGAACAATTAAGTGCTATGAATCATGATTCTTTAAAAAAGGAGTACATTTCAAATGAC
    TGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1086

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1292 [new locus tag: SAUSA300_RS07020 ]
  • symbol: Alr2
  • description: alanine racemase
  • length: 361
  • theoretical pI: 6.43166
  • theoretical MW: 41409.5
  • GRAVY: -0.29723

⊟Function[edit | edit source]

  • reaction:
    EC 5.1.1.1?  ExPASy
    Alanine racemase L-alanine = D-alanine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan alanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 148.9)
  • TheSEED  :
    • Diaminopimelate epimerase alternative form predicted for S.aureus (EC 5.1.1.7)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Lysine Biosynthesis DAP Pathway  Diaminopimelate epimerase alternative form predicted for S.aureus (EC 5.1.1.7)
  • PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 124.4)
    and 1 more
    no clan defined Ala_racemase_C; Alanine racemase, C-terminal domain (PF00842; HMM-score: 70.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002434
    • TAT(Tat/SPI): 0.000132
    • LIPO(Sec/SPII): 0.000473
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQFIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYNHKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADEFDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIALYGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGYGDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTFKGVGANSEQLSAMNHDSLKKEYISNDC

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: L-box (transcription termination) regulon, CodY (repression) regulon
    L-box(RNA)important in Lysine biosynthesis; transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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