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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1324 [new locus tag: SAUSA300_RS07215 ]
  • pan locus tag?: SAUPAN003864000
  • symbol: SAUSA300_1324
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1324 [new locus tag: SAUSA300_RS07215 ]
  • symbol: SAUSA300_1324
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1455106..1455810
  • length: 705
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTT
    ATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATT
    GCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTAAT
    GTCATGTTTGCTTCTATTTATTTAGCAACAGATATATTAAACGATATTTATGGGCGTAGA
    GTTGCAAAAAGAGCAGTTTGGTTAGGCTTTTCATCAACATTAATTATGATTATTGTTATG
    CAATTGTCATTACATTTTATTCCTGCACCAGAAGATATGGCACAAAAAGCATTACACGCA
    ATCTTTGATGTTGTGCCACGCATTGCTTTAGGCTCAATCGTCGCATATATTATTGGTCAA
    CATATTGATGTATTTATCTTTTCACTAATTAAAAAAGTATTTAGTTCTGATAAAACGTTT
    TTCATCCGTGCATATGGTAGTACATTTTTAAGCTCAATAATTGATACAGCTTTATTTGTA
    GCTATCGCATTTATCGGAAGTTTACCTGGTACAGTTGTATTTGAAATATTTATTACAACG
    TACGTATTAAAATTAGCTTCAACAGTTTTCAATGTACCATTTGGATATATTGCTAAATCA
    TTTTATCGTAAAGGTAAGATTGATAAGTTAGATCAAGGCTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1324 [new locus tag: SAUSA300_RS07215 ]
  • symbol: SAUSA300_1324
  • description: hypothetical protein
  • length: 234
  • theoretical pI: 9.87834
  • theoretical MW: 26030.9
  • GRAVY: 0.900855

⊟Function[edit | edit source]

  • TIGRFAM:
    Hypothetical proteins Conserved conserved hypothetical integral membrane protein (TIGR00697; HMM-score: 192.9)
  • TheSEED  :
    • Putative preQ0 transporter
    RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  Putative preQ0 transporter
  • PFAM:
    no clan defined Vut_1; Putative vitamin uptake transporter (PF02592; HMM-score: 147.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003695
    • TAT(Tat/SPI): 0.00014
    • LIPO(Sec/SPII): 0.00267
  • predicted transmembrane helices (TMHMM): 7

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQVIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQLSLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTFFIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIAKSFYRKGKIDKLDQGY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PreQ1 riboswitch (transcription termination) regulon
    PreQ1 riboswitch(RNA)important in Queuosine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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