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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1326 [new locus tag: SAUSA300_RS07230 ]
  • pan locus tag?: SAUPAN003871000
  • symbol: SAUSA300_1326
  • pan gene symbol?: rnhA
  • synonym:
  • product: putative cell wall enzyme EbsB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1326 [new locus tag: SAUSA300_RS07230 ]
  • symbol: SAUSA300_1326
  • product: putative cell wall enzyme EbsB
  • replicon: chromosome
  • strand: +
  • coordinates: 1456351..1456752
  • length: 402
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGGCGAAAATAAATTTTGATGCTGCGACGAAAGGAAATCCAGGCATAAGTACATGTGCC
    ATTGTAATCAAAGAAGATGAGCAGCATTATACATATACACATGAGTTAGGCGAAATGGAT
    AACCACACTGCAGAATGGGCTGCATGTATTTATGCACTAGAACATGCACGTGAATTAAAT
    GTTCAAAACGCACTATTATATACAGACTCAAAGCTAATTGCAGATAGCATTGAAGCTGGT
    TATGTGAAAAACGCAAATTTCAAACCTTATTTTGATCAAATAGAAATATTTGAAAAAGAT
    TTTGATTTATTATTTGTTAAATGGATACCGAGAGAACAAAACAAAGAAGCGAATCAACAC
    GCTCAACAGGCATTGTATAAATTAATTAAAAAGAATAAATAA
    60
    120
    180
    240
    300
    360
    402

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1326 [new locus tag: SAUSA300_RS07230 ]
  • symbol: SAUSA300_1326
  • description: putative cell wall enzyme EbsB
  • length: 133
  • theoretical pI: 5.58782
  • theoretical MW: 15354.2
  • GRAVY: -0.529323

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • RNase H
  • PFAM:
    RNase_H (CL0219) RVT_3; Reverse transcriptase-like (PF13456; HMM-score: 61)
    and 1 more
    RNase_H; RNase H (PF00075; HMM-score: 31.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.2
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007292
    • TAT(Tat/SPI): 0.000152
    • LIPO(Sec/SPII): 0.004787
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTAEWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFDLLFVKWIPREQNKEANQHAQQALYKLIKKNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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