PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1334 [new locus tag: SAUSA300_RS07270 ]
• pan locus tag^?: SAUPAN003897000
• symbol: SAUSA300_1334
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1334 [new locus tag: SAUSA300_RS07270 ]
• symbol: SAUSA300_1334
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1498745..1500085
• length: 1341
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913297 NCBI
• RefSeq: YP_494031 NCBI
• BioCyc: see SAUSA300_RS07270
• MicrobesOnline: 1292849 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAAAATACATTTAATCCATTACAAAGATTACATTTCTATGCAGCAATATTTATTGCT
  CCACTGTTAATCACTTTAACCATTTCGGGCATTGGTTACTTGTTCTTTCCAGAAGTTGAA
  AATAATATTTATAAGAATGAGTTTTTTGGTGACAGTGATGTAAAAACGCATCAAACATTA
  AATGATGCAGTACATCAAGTTGAACAACAATATGAAGGATTCTTTGTAAGTAAAGTTAGC
  ATACTTGATGAACCATATAACAAACGAATTACACTGAGTGATATGGCAGGAAATCAACGT
  TACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTAT
  TCTAATGTGATGCGAAGTATACATAGTTCTTTGTTTACTGAAAATACTATTATTAATTAT
  TTAGTAGAGTTAACCGCATGTTGGATGATATTCATGATTTTATCTGGTACTTATTTACTC
  ATTAAGAAGCATTTAATTTCTAACAAAAGTAAGGCACTTCGTTGGCAAAAGTGGCACGCA
  ATGATTGGAGTTATCATTGCAATTCCAGTATTTGTATTAGTCTTAACTGGATTGCCATGG
  TCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGCCCTTGGTCAA
  GGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGT
  AAAAATAAACAGCCAGCTTCATCCGAAAAAGGTTCAAGTGGTCATCATGGTAATGCAGCA
  ATGCCTCAAACCAAATTAGATTATCAAATATCTATTGATAAGGTCGTTGAACAGGCGCAA
  AAAGCTGGTATTAAAAAGCCGTTTTCAATCGTATATCCAAGTGATAAAAATGGTACCTTT
  ATTGTATCTAATACTAGTAATTCAGGTGTTACTGGGCTAGATGTATCACCATACAAGGAA
  CAAACACTTTATTTCGATCAATATAGCGGTAAAAAGCTAGGTACGATTAAATATGATGAC
  TACGGTATTATTGCTAAATGGTTTACATGGGGCATTCCGCTTCACGAAGGTCATTTATTC
  GGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGC
  ATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGC
  GTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATG
  CCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAA
  GATCGTCGTGCTAAACAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1334 [new locus tag: SAUSA300_RS07270 ]
• symbol: SAUSA300_1334
• description: hypothetical protein
• length: 446
• theoretical pI: 9.82061
• theoretical MW: 50171.3
• GRAVY: 0.0874439

⊟Function

• TIGRFAM:
  TIGR03943 family protein (TIGR03943; HMM-score: 9.9)
• TheSEED  :
  • Iron-uptake factor PiuB
  • Uncharacterized iron-regulated membrane protein
• PFAM:
  PepSY_TM-like (CL0490) PepSY_TM; PepSY-associated TM region (PF03929; HMM-score: 177.1)
  and 5 more
  no clan defined SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 12.6)
  Peptidase_A8; Signal peptidase (SPase) II (PF01252; HMM-score: 9.7)
  TctB; Tripartite tricarboxylate transporter TctB family (PF07331; HMM-score: 8.6)
  Cytochrom_B_C; Cytochrome b(C-terminal)/b6/petD (PF00032; HMM-score: 7.2)
  DUF1980; Domain of unknown function (DUF1980) (PF09323; HMM-score: 7.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.123574
  • TAT(Tat/SPI): 0.000974
  • LIPO(Sec/SPII): 0.036904
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 87160073 NCBI
• RefSeq: YP_494031 NCBI
• UniProt: A0A0H2XFR3 UniProt

⊟Protein sequence

• MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIYKNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYVFLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLLIKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGALGQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVVEQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLGTIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKNTAIKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications