PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1453 [new locus tag: SAUSA300_RS07935 ]
• pan locus tag^?: SAUPAN004037000
• symbol: SAUSA300_1453
• pan gene symbol^?: rnz
• synonym:
• product: ribonuclease Z

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1453 [new locus tag: SAUSA300_RS07935 ]
• symbol: SAUSA300_1453
• product: ribonuclease Z
• replicon: chromosome
• strand: -
• coordinates: 1605755..1606675
• length: 921
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914426 NCBI
• RefSeq: YP_494149 NCBI
• BioCyc: see SAUSA300_RS07935
• MicrobesOnline: 1292968 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGGAAGTTACATTTTTTGGAACGAGTGCAGGTTTGCCTACAAAAGAGAGAAATACACAA
  GCAATCGCCTTAAATTTAGAACCATATTCCAATTCCATATGGCTTTTCGACGTTGGTGAA
  GGTACACAGCACCAAATTTTACATCATGCAATTAAATTAGGAAAAGTGACACATATATTT
  ATTACTCATATGCATGGCGATCATATTTTTGGTTTGCCAGGATTACTTTCTAGTCGTTCT
  TTTCAGGGCGGTGAACAGAAGCCGCTTACATTGGTTGGACCAAAAGGAATTAAAGCATAT
  GTGGAAATGTCTATGAATTTATCAGAGTCACATTTAAACTATCCAATTACATATATTGAA
  ATCGATGATCATCTGACATATCATCATGATGGTTTTACTGTAGAGGCGCATTTACTTAAT
  CATGGTATTCCATCATATGGATATAGAGTAATGGCACCTGAAACTACAGGTACAATCAAT
  GTTGAAGCGTTAAAAAACATTGGTCTTGAACCAGGTCCTAAATATCAAGAAGTAAAATCT
  CATGATACATTTGAACATAATGGTCAAGTTTATCAATCAAAAGATTTTAGAGGTGAGTCC
  AAACAGGGTCCAGTTGTAGCAATTTTTGGTGATACAAAACCATGTTCTAATGAACGTGTG
  ATTTCTAGAGATGCTGATGTGATGGTACATGAAGCGACCTATATAGATGGTGAAAAGCAT
  TTAGCCAACAATTATCATCATAGCCATATTGAAGACGTATTTGCATTGATAAAAGAAGCC
  AATGTTAAACGTACGTTGATTACACATTTGAGCAATCGCTATAATACAGAAGATATAAAT
  GAAATTTATCAAACATTGATACAAAATGAAGATACACCTAATTTTAATTTTGTAAAAGAT
  TTTGATAGTTTTAAAATCTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  921
  

⊟Protein

⊟General

• locus tag: SAUSA300_1453 [new locus tag: SAUSA300_RS07935 ]
• symbol: SAUSA300_1453
• description: ribonuclease Z
• length: 306
• theoretical pI: 6.22105
• theoretical MW: 34584.5
• GRAVY: -0.446078

⊟Function

• reaction:
  EC 3.1.26.11?  ExPASy
  Ribonuclease Z Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule
• TIGRFAM:
  Genetic information processing Transcription RNA processing ribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 338.2)
  and 5 more
  Genetic information processing Transcription RNA processing ribonuclease BN (TIGR02649; EC 3.1.-.-; HMM-score: 251.7)
  Genetic information processing Transcription RNA processing ribonuclease Z, Thermotoga type (TIGR02650; EC 3.1.26.11; HMM-score: 56.4)
  Cellular processes Cellular processes Detoxification hydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 15.1)
  Unknown function Enzymes of unknown specificity quinoprotein relay system zinc metallohydrolase 2 (TIGR04559; EC 3.-.-.-; HMM-score: 12.9)
  phosphonate metabolism protein PhnP (TIGR03307; HMM-score: 12.2)
• TheSEED  :
  • Ribonuclease Z (EC 3.1.26.11)
  RNA Metabolism RNA processing and modification tRNA processing  Ribonuclease Z (EC 3.1.26.11)
• PFAM:
  Metallo-HOrase (CL0381) Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 54.3)
  and 3 more
  Lactamase_B_4; tRNase Z endonuclease (PF13691; HMM-score: 33)
  Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 31.7)
  no clan defined MBF2; Transcription activator MBF2 (PF15868; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007937
  • TAT(Tat/SPI): 0.000348
  • LIPO(Sec/SPII): 0.001189
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159998 NCBI
• RefSeq: YP_494149 NCBI
• UniProt: Q2FGM9 UniProt

⊟Protein sequence

• MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQHQILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVEMSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTINVEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNERVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNTEDINEIYQTLIQNEDTPNFNFVKDFDSFKI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications