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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1565 [new locus tag: SAUSA300_RS08535 ]
  • pan locus tag?: SAUPAN004204000
  • symbol: SAUSA300_1565
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1565 [new locus tag: SAUSA300_RS08535 ]
  • symbol: SAUSA300_1565
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1714662..1715576
  • length: 915
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATCAATTTAGTTTTAAGGTTGCGCAGTCATTAATTAACAATGATGGTCCAGCGATT
    GAATATACTTTGATTGGTCCTACCATTCAATTTAATACGCAAAATACATTTGTTATAACC
    GGTGGTAGTATTAATGCTTCGCTAAATAATAAAACTATATCAATGAATTCTGTCATATTA
    GCTGAGAAAGGTGACATTTTAAAAATAGGTGCTATAACCAAAGGTGCACGCGGTTATCTT
    ACTTTTGGTCATTCTATCAACGTACCTTCTATTGCTGAAAGTTATGCAACACATACCCGA
    AGCAGTATAGGAGGATTCAAAGGTAGAAAGTTATTAGCTGATGATGTAATAACAGTGAAA
    ATAAATAATGACTTTAAAGAAAACATTGGAAAGACGATTCATTTACAGGACGATTTATTG
    CCAGAAAATAATATTATACACATTCTTCAAGGACCTCAATTCGAGGCATTTTCTGAAGAG
    GCTAGAGCGAAAATTGTAAATCATCCATATTTAATTACTGAACAATCAGACCGTATGGGC
    TATCGTTTGGAAGGTGACAGCGTTGCACCAATTAATCAAGCAGATATCATTTCTGAACCG
    GTTGCTCTTGGTAGTGTTCAGGTACCAAATGATGGTCAACCTATTATTCTGCTTAATGAT
    AAACAAACGATAGGTGGTTATACCAAAATTGCAACTGTATGCAAATTTGATCTGCCAAAG
    TTAGCACAGATGAAACCACAAGATACAATTCAATTTAAATGGATATCATTCCAAGAAGCT
    GTAGATAAGAATCGGGAACAAATGTCTCTGTTTAATGAAATTCTTAAGAGTCATCAAAAG
    ACTCCAATTTTTGATACATCTAGCTTGCGACATACTTCGAAAAAATTAGCAACAATTTTA
    AAAGGGGATTTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1565 [new locus tag: SAUSA300_RS08535 ]
  • symbol: SAUSA300_1565
  • description: hypothetical protein
  • length: 304
  • theoretical pI: 7.23484
  • theoretical MW: 33556.1
  • GRAVY: -0.232895

Function[edit | edit source]

  • TIGRFAM:
    Unknown function General biotin-dependent carboxylase uncharacterized domain (TIGR00724; HMM-score: 224.6)
    and 1 more
    Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 138.3)
  • TheSEED  :
    • prokaryotic 5-oxoprolinase C, pxpC
    Allophanate hydrolase 2 and Biotin carboxylase cluster  Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54)
    and 1 more
    EC699-706  Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54)
  • PFAM:
    Cyclophil-like (CL0475) CT_A_B; Carboxyltransferase domain, subdomain A and B (PF02626; HMM-score: 248.6)
    and 1 more
    no clan defined DUF3660; Receptor serine/threonine kinase (PF12398; HMM-score: 12.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012117
    • TAT(Tat/SPI): 0.000416
    • LIPO(Sec/SPII): 0.000534
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDQFSFKVAQSLINNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIGAITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKENIGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGDSVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQMKPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKGDL

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]