PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1642 [new locus tag: SAUSA300_RS08960 ]
• pan locus tag^?: SAUPAN004321000
• symbol: SAUSA300_1642
• pan gene symbol^?: aapA
• synonym:
• product: D-serine/D-alanine/glycine transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1642 [new locus tag: SAUSA300_RS08960 ]
• symbol: SAUSA300_1642
• product: D-serine/D-alanine/glycine transporter
• replicon: chromosome
• strand: +
• coordinates: 1801077..1802438
• length: 1362
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913760 NCBI
• RefSeq: YP_494337 NCBI
• BioCyc: see SAUSA300_RS08960
• MicrobesOnline: 1293157 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGCTGAAAAATTACAAAGGGAACTGAGCAATCGCCACATACAATTAATTGCAATTGGC
  GGTGCAATTGGTACAGGCTTATTCTTAGGTGCTGGTCAAACGATTGCATTAACCGGCCCT
  TCAATTCTATTAACATACATCATTATAGGATTTATGTTATTTATGTTTATGCGAGGTTTA
  GGAGAAATCATTATACAGAATACTGAATTTAAATCTTTCGCAGATGTAACCAATACATAT
  ATTGGGCCTTTTGCAGGATTTGTTACCGGATGGACATACTGGTTCTGTTGGATTATTACA
  GGTATGGCTGAAGTAACGGCTGTGGCAAAATATGTTAGCTTTTGGTTCCCAGAAATTCCA
  AACTGGATAAGTGCACTATTTTGTGTACTGTTATTAATGTCATTCAACCTACTTAGCGCA
  AGACTTTTCGGAGAATTAGAATTTTGGTTCTCTATCATTAAAATAGCGACAATTATTGGT
  TTAATAGTAGTTGGTTTCGTCATGATTCTATTTGCATTTAAAACTCAATTCGGGCATGCC
  AGTTTCACAAATCTATATGAACACGGCATATTCGCTAAAGGTGCTTCTGGATTCTTTATG
  TCTTTCCAAATGGCACTATTCTCATTTGTAGGAATTGAAATGATTGGTGTTACAGCTGGG
  GAAACAAAAGATCCAGTTAAAACAATTCCAAAAGCAATTAACAGTGTACCCATTAGAATT
  TTAATATTTTACGTTGGGGCGTTAGCGGTTATCATGTCTATTATCCCTTGGCAGCAAGTT
  GATCCTGATAACAGTCCATTCGTAAAATTATTCGCATTGATCGGAATTCCGTTTGCTGCG
  GGCTTGATTAATTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTC
  TCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCT
  AAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTT
  GTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACT
  ACCATCTCTACAGTGTTATTTTTAGTTGTATGGGGTCTGATTATCATTGCATATATCAAT
  TATAGTCGTAAAAACCCAGATCTACATAAAAATGCTACGTACAAACTATTAGGTGGCAAA
  TATATGGGCTACTTAATATTTGTATTCTTCATTTTTGTGTTCGGGTTATTATTTATTAAT
  GTTGATACAAGACGTGCAATTTATTTTATTCCGATTTGGTTTATACTTTTAGCATTTATG
  TACTTAAGATATAAACGTATCGCTGCTAAATCAAATAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1642 [new locus tag: SAUSA300_RS08960 ]
• symbol: SAUSA300_1642
• description: D-serine/D-alanine/glycine transporter
• length: 453
• theoretical pI: 9.92242
• theoretical MW: 50480.7
• GRAVY: 0.80883

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 317.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 265.7)
  and 10 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 109.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 81.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 71.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 69.9)
  histidine-histamine antiporter (TIGR04298; HMM-score: 54.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 41)
  Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 40.8)
  putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 38.8)
  putrescine-ornithine antiporter (TIGR04299; HMM-score: 18.1)
  Metabolism Energy metabolism Electron transport cytochrome c oxidase, subunit II (TIGR02866; EC 1.9.3.1; HMM-score: 11.1)
• TheSEED  :
  • D-serine/D-alanine/glycine transporter
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  D-serine/D-alanine/glycine transporter
  and 1 more
  Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  D-serine/D-alanine/glycine transporter
• PFAM:
  APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 339.5)
  and 2 more
  AA_permease_2; Amino acid permease (PF13520; HMM-score: 147)
  Transporter (CL0375) Amastin; Amastin surface glycoprotein (PF07344; HMM-score: 9.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006878
  • TAT(Tat/SPI): 0.009037
  • LIPO(Sec/SPII): 0.056094
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 87160222 NCBI
• RefSeq: YP_494337 NCBI
• UniProt: A0A0H2XG72 UniProt

⊟Protein sequence

• MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILLTYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGMAEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIGLIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVTAGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGIPFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFASSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNATYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAKSNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications