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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1642 [new locus tag: SAUSA300_RS08960 ]
  • pan locus tag?: SAUPAN004321000
  • symbol: SAUSA300_1642
  • pan gene symbol?: aapA
  • synonym:
  • product: D-serine/D-alanine/glycine transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1642 [new locus tag: SAUSA300_RS08960 ]
  • symbol: SAUSA300_1642
  • product: D-serine/D-alanine/glycine transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 1801077..1802438
  • length: 1362
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCTGAAAAATTACAAAGGGAACTGAGCAATCGCCACATACAATTAATTGCAATTGGC
    GGTGCAATTGGTACAGGCTTATTCTTAGGTGCTGGTCAAACGATTGCATTAACCGGCCCT
    TCAATTCTATTAACATACATCATTATAGGATTTATGTTATTTATGTTTATGCGAGGTTTA
    GGAGAAATCATTATACAGAATACTGAATTTAAATCTTTCGCAGATGTAACCAATACATAT
    ATTGGGCCTTTTGCAGGATTTGTTACCGGATGGACATACTGGTTCTGTTGGATTATTACA
    GGTATGGCTGAAGTAACGGCTGTGGCAAAATATGTTAGCTTTTGGTTCCCAGAAATTCCA
    AACTGGATAAGTGCACTATTTTGTGTACTGTTATTAATGTCATTCAACCTACTTAGCGCA
    AGACTTTTCGGAGAATTAGAATTTTGGTTCTCTATCATTAAAATAGCGACAATTATTGGT
    TTAATAGTAGTTGGTTTCGTCATGATTCTATTTGCATTTAAAACTCAATTCGGGCATGCC
    AGTTTCACAAATCTATATGAACACGGCATATTCGCTAAAGGTGCTTCTGGATTCTTTATG
    TCTTTCCAAATGGCACTATTCTCATTTGTAGGAATTGAAATGATTGGTGTTACAGCTGGG
    GAAACAAAAGATCCAGTTAAAACAATTCCAAAAGCAATTAACAGTGTACCCATTAGAATT
    TTAATATTTTACGTTGGGGCGTTAGCGGTTATCATGTCTATTATCCCTTGGCAGCAAGTT
    GATCCTGATAACAGTCCATTCGTAAAATTATTCGCATTGATCGGAATTCCGTTTGCTGCG
    GGCTTGATTAATTTTGTAGTATTAACCGCTGCTGCTTCATCATGTAACAGTGGTATATTC
    TCAAATAGCCGTATGCTTTTCGGTTTATCAAGTCAACAACAAGCACCTCCGAACTTTTCT
    AAGACGAATAAATATGGCGTTCCACATGTTGCAATCTTTGCTTCATCAGCATTATTACTT
    GTGGCAGCATTACTAAACTATATTTTCCCAGATGCGACAAAAGTATTTACGTATGTGACT
    ACCATCTCTACAGTGTTATTTTTAGTTGTATGGGGTCTGATTATCATTGCATATATCAAT
    TATAGTCGTAAAAACCCAGATCTACATAAAAATGCTACGTACAAACTATTAGGTGGCAAA
    TATATGGGCTACTTAATATTTGTATTCTTCATTTTTGTGTTCGGGTTATTATTTATTAAT
    GTTGATACAAGACGTGCAATTTATTTTATTCCGATTTGGTTTATACTTTTAGCATTTATG
    TACTTAAGATATAAACGTATCGCTGCTAAATCAAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1362

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1642 [new locus tag: SAUSA300_RS08960 ]
  • symbol: SAUSA300_1642
  • description: D-serine/D-alanine/glycine transporter
  • length: 453
  • theoretical pI: 9.92242
  • theoretical MW: 50480.7
  • GRAVY: 0.80883

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 317.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 265.7)
    and 10 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 109.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 81.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 71.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 69.9)
    histidine-histamine antiporter (TIGR04298; HMM-score: 54.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 41)
    Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 40.8)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 38.8)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 18.1)
    Metabolism Energy metabolism Electron transport cytochrome c oxidase, subunit II (TIGR02866; EC 1.9.3.1; HMM-score: 11.1)
  • TheSEED:  
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  D-serine/D-alanine/glycine transporter
    and 1 more
    Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  D-serine/D-alanine/glycine transporter
  • PFAM:
    APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 339.5)
    and 2 more
    AA_permease_2; Amino acid permease (PF13520; HMM-score: 147)
    Transporter (CL0375) Amastin; Amastin surface glycoprotein (PF07344; HMM-score: 9.3)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006878
    • TAT(Tat/SPI): 0.009037
    • LIPO(Sec/SPII): 0.056094
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILLTYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGMAEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIGLIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVTAGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGIPFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFASSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNATYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAKSNK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • SAUSA300_1642 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]