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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1714 [new locus tag: SAUSA300_RS09365 ]
  • pan locus tag?: SAUPAN004457000
  • symbol: ribE
  • pan gene symbol?: ribB
  • synonym:
  • product: riboflavin synthase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1714 [new locus tag: SAUSA300_RS09365 ]
  • symbol: ribE
  • product: riboflavin synthase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 1896589..1897221
  • length: 633
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTTACTGGCATCGTTGAAGAAATAGGTGTCGTTAAAAGTGTTCAAATTCGTCAATCA
    GTCAGGACGATTGAAATTGAAGCACATAAGATTACGGCAGATATGCATATTGGTGATTCC
    ATCAGTGTTAATGGTGCATGTTTAACAGTGATTGATTTTAATCAGACATCTTTTACTGTT
    CAAGTAATTAAAGGCACTGAAAATAAAACCTATTTAGCAGATGTTAAGCGACAATCAGAA
    GTAAATTTAGAGCGTGCCATGAGTGGTAACGGTAGGTTTGGTGGACATTTTGTCCTCGGT
    CATGTAGATGAACTAGGAACAGTTTCAAAAATAAATGAAACAGCCAATGCAAAAATTATT
    ACGATTCAATGTAGCCAACATATTAATAATCAGTTAGTTAAGCAAGGGTCTATTACTGTG
    GATGGTGTTAGTCTAACGGTATTTGATAAGCATGATAACAGTTTTGACATTCATCTTATT
    CCAGAAACGAGGCGTTCAACGATTTTATCATCCAAAAAATTAGGAGATAAAGTACATTTA
    GAAACAGACGTTTTGTTTAAATATGTTGAAAATATTTTAAATAAAGATAAAGACCAATTA
    TCTGTAGATAAATTAAGAGCATTTGGTTTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1714 [new locus tag: SAUSA300_RS09365 ]
  • symbol: RibE
  • description: riboflavin synthase subunit alpha
  • length: 210
  • theoretical pI: 6.96174
  • theoretical MW: 23298.4
  • GRAVY: -0.160476

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.9?  ExPASy
    Riboflavin synthase 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD riboflavin synthase, alpha subunit (TIGR00187; EC 2.5.1.9; HMM-score: 217.3)
  • TheSEED  :
    • Riboflavin synthase alpha chain (EC 2.5.1.9)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Riboflavin, FMN, FAD riboflavin to FAD  Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9)
  • PFAM:
    FAD_Lum_binding (CL0076) Lum_binding; Lumazine binding domain (PF00677; HMM-score: 144.1)
    and 2 more
    DHFred (CL0387) RibD_C; RibD C-terminal domain (PF01872; HMM-score: 14)
    no clan defined UAE_UbL; Ubiquitin/SUMO-activating enzyme ubiquitin-like domain (PF14732; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006408
    • TAT(Tat/SPI): 0.00035
    • LIPO(Sec/SPII): 0.000778
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVNGACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHVDELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLIPETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: FMN-box (transcription termination) regulon
    FMN-box(RNA)important in Riboflavin biosynthesis; transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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