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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1887 [new locus tag: SAUSA300_RS10325 ]
  • pan locus tag?: SAUPAN004935000
  • symbol: pcrB
  • pan gene symbol?: pcrB
  • synonym:
  • product: geranylgeranylglyceryl phosphate synthase-like protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1887 [new locus tag: SAUSA300_RS10325 ]
  • symbol: pcrB
  • product: geranylgeranylglyceryl phosphate synthase-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2051651..2052343
  • length: 693
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTATGACATTAAAAAATGGCGCCATATTTTTAAATTAGACCCAGCTAAACATATTTCA
    GATGATGATTTAGATGCGATTTGTATGTCTCAAACAGATGCAATTATGATTGGTGGAACT
    GATGACGTTACTGAAGATAATGTCATTCATTTAATGAGCAGAGTAAGAAGATACCCATTG
    CCTTTAGTACTTGAAATATCAAACATCGAAAGTGTAATGCCTGGTTTTGATTTTTATTTT
    GTACCTACAGTACTGAACAGTACAGATGTTGTATTTCACAATGGTACATTATTAGAAGCG
    CTTAAAACATATGGACATAGTATAGATTTTGAGGAAGTAATATTTGAAGGGTATGTCGTG
    TGCAATGCTGATAGCAAAATGGCAAAACATACCAAAGCAAATACAGATTTAACAACAGAA
    GATTTAGAAGCATATGCCCAAATGGTCAATCATATGTATCGATTACCGGTTATGTATATA
    GAGTATAGTGGCATTTATGGCGACGTATCAAAGGTTCAAGCTGTCTCAGAACATCTAACA
    GAAACGCAACTTTTTTATGGTGGCGGTATTTCCTCAGAACAACAAGCGACAGAGATGGCA
    GCTATTGCAGATACAATTATCGTCGGTGATATTATTTATAAAGATATTAAAAAAGCTTTA
    AAAACAGTAAAAATAAAGGAGTCTAGTAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    693

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1887 [new locus tag: SAUSA300_RS10325 ]
  • symbol: PcrB
  • description: geranylgeranylglyceryl phosphate synthase-like protein
  • length: 230
  • theoretical pI: 4.67385
  • theoretical MW: 25920.4
  • GRAVY: -0.104783

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.n9?  ExPASy
    Heptaprenylglyceryl phosphate synthase All-trans-heptaprenyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(all-trans-heptaprenyl)glycerol 1-phosphate
  • TIGRFAM:
    phosphoglycerol geranylgeranyltransferase (TIGR04146; EC 2.5.1.41; HMM-score: 243.1)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 202.7)
    and 3 more
    phosphoglycerol geranylgeranyltransferase, putative (TIGR04147; EC 2.5.1.41; HMM-score: 185.7)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 45.5)
    HAD superfamily (subfamily IG) hydrolase, 5'-nucleotidase (TIGR02244; HMM-score: 17.4)
  • TheSEED  :
    • Geranylgeranylglyceryl phosphate synthase-like protein YerE
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  (S)-3-O-geranylgeranylglyceryl phosphate synthase
  • PFAM:
    TIM_barrel (CL0036) PcrB; PcrB family (PF01884; HMM-score: 230.7)
    and 3 more
    Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 21.7)
    HAD (CL0137) 5_nucleotid; 5' nucleotidase family (PF05761; HMM-score: 13.8)
    TIM_barrel (CL0036) His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002072
    • TAT(Tat/SPI): 0.000145
    • LIPO(Sec/SPII): 0.000433
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVTEDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALKTYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYIEYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKKALKTVKIKESSK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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