From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1900 [new locus tag: SAUSA300_RS10400 ]
  • pan locus tag?: SAUPAN004954000
  • symbol: ppaC
  • pan gene symbol?: ppaC
  • synonym:
  • product: putative manganese-dependent inorganic pyrophosphatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1900 [new locus tag: SAUSA300_RS10400 ]
  • symbol: ppaC
  • product: putative manganese-dependent inorganic pyrophosphatase
  • replicon: chromosome
  • strand: +
  • coordinates: 2066320..2067249
  • length: 930
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCTAAAACATATATTTTCGGACATAAGAATCCAGACACTGATGCAATTTCATCTGCG
    ATTATTATGGCAGAATTTGAACAACTTCGAGGTAATTCAGGAGCCAAAGCATACCGTTTA
    GGTGATGTGAGTGCAGAAACTCAATTCGCGTTAGATACATTTAATGTACCTGCTCCGGAA
    TTATTAACAGATGATTTAGATGGTCAAGATGTTATCTTAGTTGATCATAACGAATTCCAA
    CAAAGTTCTGATACGATTGCCTCTGCTACAATTAAGCATGTAATTGATCATCACAGAATT
    GCAAATTTCGAAACTGCTGGTCCTTTATGTTATCGTGCTGAACCAGTTGGTTGTACAGCT
    ACAATTTTATACAAAATGTTTAGAGAACGTGGCTTTGAAATTAAACCTGAAATTGCCGGT
    TTAATGTTATCAGCAATTATCTCAGATAGCTTACTTTTCAAATCACCAACATGTACACAA
    CAAGATGTTAAAGCAGCTGAAGAATTAAAAGATATTGCTAAAGTTGATATTCAAAAGTAC
    GGCTTAGATATGTTAAAAGCAGGTGCTTCAACAACTGATAAATCAGTTGAATTCTTATTA
    AACATGGATGCTAAATCATTTACTATGGGTGACTATGTGACTCGTATTGCACAAGTTAAT
    GCTGTTGACCTTGACGAAGTGTTAAATCGTAAAGAAGATTTAGAAAAAGAAATGTTAGCT
    GTAAGTGCACAAGAAAAATATGACTTATTTGTACTTGTTGTTACTGACATCATTAATAGT
    GATTCTAAAATTTTAGTTGTAGGTGCTGAAAAAGATAAAGTTGGCGAAGCATTCAATGTT
    CAATTAGAAGATGACATGGCCTTCTTATCTGGTGTCGTTTCTCGAAAAAAACAAATCGTA
    CCTCAAATCACTGAAGCATTAACAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    930

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1900 [new locus tag: SAUSA300_RS10400 ]
  • symbol: PpaC
  • description: putative manganese-dependent inorganic pyrophosphatase
  • length: 309
  • theoretical pI: 4.45124
  • theoretical MW: 34068.6
  • GRAVY: -0.104531

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.1.1?  ExPASy
    Inorganic diphosphatase Diphosphate + H2O = 2 phosphate
  • TIGRFAM:
  • TheSEED  :
    • Manganese-dependent inorganic pyrophosphatase (EC 3.6.1.1)
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Manganese-dependent inorganic pyrophosphatase (EC 3.6.1.1)
  • PFAM:
    HAD (CL0137) DHH; DHH family (PF01368; HMM-score: 99.9)
    and 1 more
    no clan defined DHHA2; DHHA2 domain (PF02833; HMM-score: 69.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0051
    • TAT(Tat/SPI): 0.000663
    • LIPO(Sec/SPII): 0.000633
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVSAETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIANFETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQQDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQVNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGEAFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_1900&oldid=70663"