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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1972 [new locus tag: SAUSA300_RS10835 ]
  • pan locus tag?: SAUPAN005188000
  • symbol: int
  • pan gene symbol?: int3
  • synonym:
  • product: integrase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1972 [new locus tag: SAUSA300_RS10835 ]
  • symbol: int
  • product: integrase
  • replicon: chromosome
  • strand: +
  • coordinates: 2126612..2127649
  • length: 1038
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGTATGAAGGAAAA
    AGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAG
    TTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTC
    GAAAATTGGATTAAAACGTATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTAT
    AGAAATGCATTACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATA
    AATAGACAAGTTTATCAGAAATTCATAAACGATTATTCAAAAGAACACGCAAAAGAAACT
    ATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGGCTT
    ATTTTTAAAAATCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAA
    CAAGAAAAATTCATCTCGGTAACTGAATATGAAATACTAAAAGATCACGTCAGAAAGAAG
    AGAACTCGTTCATCATTAGCGCTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGT
    GCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAG
    CGAAAAACCGATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATT
    ATGGACGTCATAAGTACATTTGCAATTAGCTATGATGGTTACATTTTCAAAGAAGCCGGA
    TCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAAT
    ATATCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAA
    GGTGTATCTATACATTACATTTCTAAAAGATTAGGTCATAAAAATATAGCAATAACTACA
    TCCGTGTATTCTCATTTGTTAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAA
    ATTTTAGAAAGTATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1038

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1972 [new locus tag: SAUSA300_RS10835 ]
  • symbol: Int
  • description: integrase
  • length: 345
  • theoretical pI: 10.2239
  • theoretical MW: 40319.1
  • GRAVY: -0.672464

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 71)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 70.5)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 42.6)
    Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 42.6)
  • TheSEED:  
    Phage integrase
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 79.4)
    and 8 more
    l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 40.4)
    Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 36.3)
    MBD-like (CL0081) Arm-DNA-bind_4; Arm DNA-binding domain (PF14657; HMM-score: 19.2)
    l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 16.1)
    no clan defined DUF1722; Protein of unknown function (DUF1722) (PF08349; HMM-score: 14.4)
    MACRO (CL0223) Macro; Macro domain (PF01661; HMM-score: 13.3)
    no clan defined NblA; Phycobilisome degradation protein nblA (PF04485; HMM-score: 13.1)
    AEP (CL0243) Replicase; Replicase family (PF03090; HMM-score: 12.4)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018998
    • TAT(Tat/SPI): 0.000588
    • LIPO(Sec/SPII): 0.00368
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNELRSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINRQVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSEQEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFIDERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSACRVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEEDKKTTKILESM

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]