PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2002 [new locus tag: SAUSA300_RS11010 ]
• pan locus tag^?: SAUPAN005298000
• symbol: SAUSA300_2002
• pan gene symbol^?: gcp
• synonym:
• product: putative DNA-binding/iron metalloprotein/AP endonuclease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2002 [new locus tag: SAUSA300_RS11010 ]
• symbol: SAUSA300_2002
• product: putative DNA-binding/iron metalloprotein/AP endonuclease
• replicon: chromosome
• strand: -
• coordinates: 2161803..2162828
• length: 1026
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912956 NCBI
• RefSeq: YP_494653 NCBI
• BioCyc: see SAUSA300_RS11010
• MicrobesOnline: 1293517 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  1021
  
  ATGACTAAAGATATATTAATACTAGCTGTTGAAACAAGTTGTGATGAAACAAGCGTTAGT
  GTTATAAAAAATGGCAGAGATATTTTATCAAATACAGTTTTAAGTCAGATTGAAAGTCAT
  AAACGATTTGGCGGTGTCGTTCCCGAAGTGGCAAGTAGACATCACGTTGAAGGTATAACA
  GCAACAATAAACGAGGCTCTAGGGGATGCCGATGTATCAATAGAAGATATTGATGCCATA
  GCGGTTACAGAAGGCCCTGGACTAATTGGTGCGTTACTAATAGGTGTTAATGCAGCCAAA
  GCATTGGCATTTGCTTACGATAAGCCACTTATTCCTGTTCATCATATTGCAGGACATATA
  TATGCTAATCACATAGAAGAGCCATTAACATTCCCGCTAATTGCACTTATTGTTTCAGGT
  GGACATACTGAATTAGTTTATATGAAGGATCATTTATCATTTGAAGTCATTGGTGAAACA
  CGAGATGACGCAGTAGGTGAGGCTTATGATAAAGTGGCACGAACAATTGGTTTAAATTAT
  CCAGGTGGTCCACAAGTTGATCGGTTGGCTGCTGAAGGTGAAGATACTTATTCATTCCCT
  CGTGTTTGGTTGGATAAAGATAGTTATGATTTTAGTTTTAGTGGGTTGAAAAGTGCCGTA
  ATCAATCAACTTCACAATCAACGACAAAAAAATATTCCAATCATTGAAGCTAACGTAGCA
  ACGAGCTTTCAAAACAGTGTTGTAGAGGTGCTCACGTTTAAAGCTATTCAAGCTTGTAAA
  GAATATGGTGTTCAGCGATTAATTGTTGCTGGTGGCGTGGCGAGTAATAAAGGATTACGT
  CAATCTTTAGCGGATCAATGCAAAGTCAATGACATTCAATTAACTATCCCAAGTCCTAAA
  TTATGCACAGATAATGCTGCAATGATAGGCGTTGCCGGCCACTATTTGTATCAGCAAGGT
  CGATTTGCTGATTTAGCATTAAATGGGCACAGCAATATAGATTTAGAAGAGTATTCTGCA
  GAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2002 [new locus tag: SAUSA300_RS11010 ]
• symbol: SAUSA300_2002
• description: putative DNA-binding/iron metalloprotein/AP endonuclease
• length: 341
• theoretical pI: 4.7955
• theoretical MW: 36818.4
• GRAVY: 0.00469208

⊟Function

• reaction:
  EC 2.3.1.234?  ExPASy
  N⁶-L-threonylcarbamoyladenine synthase L-threonylcarbamoyladenylate + adenine³⁷ in tRNA = AMP + N⁶-L-threonylcarbamoyladenine³⁷ in tRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 439.8)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides metallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 364.9)
  and 3 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair universal archaeal protein Kae1 (TIGR03722; HMM-score: 223.5)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 57.6)
  Genetic information processing Protein fate Protein modification and repair carbamoyltransferase HypF (TIGR00143; HMM-score: 18.2)
• TheSEED  :
  • TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory YgjD and YeaZ  TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
  and 2 more
  RNA Metabolism RNA processing and modification YrdC-YciO  TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
  RNA Metabolism RNA processing and modification YrdC-YciO-Sua5 and associated protein families  TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
• PFAM:
  Actin_ATPase (CL0108) Peptidase_M22; Glycoprotease family (PF00814; HMM-score: 344.7)
  and 3 more
  Carbam_trans_N; Carbamoyltransferase N-terminus (PF02543; HMM-score: 20.8)
  FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 13.8)
  NADP_Rossmann (CL0063) DUF166; Domain of unknown function (PF02593; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Fe²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008658
  • TAT(Tat/SPI): 0.001152
  • LIPO(Sec/SPII): 0.029951
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160879 NCBI
• RefSeq: YP_494653 NCBI
• UniProt: Q2FF75 UniProt

⊟Protein sequence

• MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFGGVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKALAFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGETRDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKSAVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASNKGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNIDLEEYSAE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2002 < rimI < SAUSA300_2004 < SAUSA300_2005

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications