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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2079 [new locus tag: SAUSA300_RS11445 ]
  • pan locus tag?: SAUPAN005417000
  • symbol: fba
  • pan gene symbol?: fbaA
  • synonym:
  • product: fructose-bisphosphate aldolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2079 [new locus tag: SAUSA300_RS11445 ]
  • symbol: fba
  • product: fructose-bisphosphate aldolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2241429..2242289
  • length: 861
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCCTTTAGTTTCAATGAAAGAAATGTTAATTGATGCAAAAGAAAATGGTTATGCGGTA
    GGTCAATACAATATTAATAACCTAGAATTCACTCAAGCAATTTTAGAAGCGTCACAAGAA
    GAAAATGCACCTGTAATTTTAGGTGTTTCTGAAGGTGCTGCTCGTTACATGAGCGGTTTC
    TACACAATTGTTAAAATGGTTGAAGGGTTAATGCATGACTTAAACATCACTATTCCTGTA
    GCAATCCATTTAGACCATGGTTCAAGCTTTGAAAAATGTAAAGAAGCTATCGATGCTGGT
    TTCACATCAGTAATGATCGATGCTTCACACAGCCCATTCGAAGAAAACGTAGCAACAACT
    AAAAAAGTTGTTGAATACGCTCATGAAAAAGGTGTTTCTGTAGAAGCTGAATTAGGTACT
    GTTGGTGGACAAGAAGATGATGTTGTAGCAGACGGCATCATTTATGCTGATCCTAAAGAA
    TGTCAAGAACTAGTTGAAAAAACTGGTATTGATGCATTAGCGCCAGCATTAGGTTCAGTT
    CATGGTCCATACAAAGGTGAACCAAAATTAGGATTTAAAGAAATGGAAGAAATCGGTTTA
    TCTACAGGTTTACCATTAGTATTACACGGTGGTACTGGTATCCCGACTAAAGATATCCAA
    AAAGCAATTCCATTTGGTACAGCTAAAATTAACGTAAACACTGAAAACCAAATCGCTTCA
    GCAAAAGCAGTTCGTGACGTTTTAAATAACGACAAAGAAGTTTACGATCCTCGTAAATAC
    TTAGGACCTGCACGTGAAGCCATCAAAGAAACAGTTAAAGGTAAAATTAAAGAGTTCGGT
    ACTTCTAACCGCGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    861

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2079 [new locus tag: SAUSA300_RS11445 ]
  • symbol: Fba
  • description: fructose-bisphosphate aldolase
  • length: 286
  • theoretical pI: 4.74859
  • theoretical MW: 30835.9
  • GRAVY: -0.228322

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.2.13?  ExPASy
    Fructose-bisphosphate aldolase D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis fructose-1,6-bisphosphate aldolase, class II (TIGR01859; EC 4.1.2.13; HMM-score: 427.6)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis ketose-bisphosphate aldolase (TIGR00167; HMM-score: 379.5)
    and 3 more
    class II aldolase, tagatose bisphosphate family (TIGR01858; EC 4.1.2.-; HMM-score: 289.7)
    fructose-bisphosphate aldolase, class II, Calvin cycle subtype (TIGR01521; EC 4.1.2.13; HMM-score: 193)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis fructose-bisphosphate aldolase, class II (TIGR01520; EC 4.1.2.13; HMM-score: 104.2)
  • TheSEED  :
    • Fructose-bisphosphate aldolase class II (EC 4.1.2.13)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  Fructose-bisphosphate aldolase class II (EC 4.1.2.13)
    and 1 more
    Carbohydrates One-carbon Metabolism Formaldehyde assimilation: Ribulose monophosphate pathway  Fructose-bisphosphate aldolase class II (EC 4.1.2.13)
  • PFAM:
    TIM_barrel (CL0036) F_bP_aldolase; Fructose-bisphosphate aldolase class-II (PF01116; HMM-score: 341.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006066
    • TAT(Tat/SPI): 0.000257
    • LIPO(Sec/SPII): 0.00049
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAPVILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFTSVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKECQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKDIQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKIKEFGTSNRAK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_0993(pdhA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    SAUSA300_1134(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SAUSA300_0533(tuf)elongation factor Tu  [1] (data from MRSA252)
    SAUSA300_0658LysR family transcriptional regulator  [1] (data from MRSA252)
    SAUSA300_0668hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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