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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2109
  • pan locus tag?: SAUPAN005501000
  • symbol: fmtB
  • pan gene symbol?: fmtB
  • synonym:
  • product: truncated FmtB protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2109
  • symbol: fmtB
  • product: truncated FmtB protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2278257..2282138
  • length: 3882
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    GTGACACAAGCAAATAGCAACATTGAAGCTGCTAATAGTCAAAATGATGTAGACCAAGCG
    AAAACGACAGGTGAAAATAGTATTGATCAAGTAACACCAACAGTTAATAAAAAAGCAACT
    GCACGTAATGAAATCACAGCAATTTTAAATAACAAATTGCAAGAGATTCAAGCTACGCCA
    GATGCAACAGATGAAGAAAAACAAGCAGCTGATGCTGAAGCAAATACTGAAAATGGTAAA
    GCAAATCAAGCCATTTCAGCAGCAACTACTAACGCACAAGTTGATGAAGCTAAAGCAAAT
    GCAGAAGCAGCGATTAATGCGGTAACACCAAAAGTTGTGAAGAAACAAGCGGCTAAAGAT
    GAAATTGATCAATTACAAGCAACGCAAACAAATGTTATCAATAATGATCAGAACGCTACA
    ACAGAAGAAAAAGAAGCAGCTATTCAACAATTAGCAACAGCAGTTACAGACGCGAAAAAT
    AATATTACAGCTGCAACTGATGATAATGGTGTAGATCAGGCGAAAGACGCTGGAAAGAAT
    TCAATTCAAAGCACGCAACCAGCAACAGCGGTTAAATCAAATGCTAAAAATGATGTTGAT
    CAAGCTGTGACAACTCAAAATCAAGCAATTGATAATACAACTGGTGCTACAACTGAAGAG
    AAAAATGCAGCAAAAGATTTAGTTTTAAAAGCTAAAGAAAAAGCGTATCAAGATATCTTA
    AATGCACAAACAACTAATGATGTTACGCAAATTAAAGATCAAGCAGTTGCTGATATTCAA
    GGTATTACTGCAGATACAACAATTAAAGATGTTGCGAAAGATGAATTAGCAACAAAAGCA
    AACGAACAAAAAGCGCTTATTGCACAAACTGCAGATGCGACTACTGAAGAAAAAGAACAA
    GCAAATCAACAAGTAGACGCACAATTAACACAAGGTAATCAAAATATTGAAAATGCACAG
    TCAATCGATGATGTAAACACTGCAAAAGATAATGCAATTCAAGCAATTGACCCAATTCAA
    GCATCAACAGATGTTAAAACGAATGCAAGAGCGGAATTGCTAACTGAAATGCAAAATAAA
    ATAACTGAAATACTTAATAATAATGAGACTACTAATGAAGAAAAAGGTAACGATATTGGA
    CCAGTTAGAGCAGCATATGAAGAAGGTTTAAATAATATTAATGCAGCAACTACTACAGGT
    GATGTAACTACTGCTAAAGATACAGCAGTACAAAAAGTTCAACAACTTCATGCAAATCCT
    GTTAAGAAACCAGCAGGTAAAAAAGAATTAGATCAAGCTGCAGCTGATAAGAAAACACAA
    ATAGAACAAACACCAAATGCATCACAACAAGAAATTAATGATGCAAAACAAGAAGTTGAT
    ACTGAATTAAATCAAGCGAAAACAAATGTCGATCAATCATCAACAAATGAATATGTTGAT
    AATGCAGTTAAAGAAGGAAAAGCTAAAATTAATGCAGTTAAAACATTTAGTGAGTACAAA
    AAAGATGCTTTAGCTAAAATTGAAGATGCATATAATGCTAAAGTAAACGAAGCGGATAAC
    TCTAACGCATCGACTTCAAGTGAAATTGCTGAAGCGAAACAAAAACTTGCTGAATTAAAA
    CAAACTGCGGATCAAAATGTTAATCAAGCTACTTCTAAAGATGACATTGAAGTTCAAATT
    CATAATGACTTAGATAATATTAACGATTACACAATTCCAACAGGTAAAAAAGAATCAGCT
    ACAACAGATTTATATGCTTATGCAGATCAGAAGAAAAATAATATTTCAGCTGACACTAAT
    GCAACACAAGATGAAAAGCAACAAGCAATTAAGCAAGTTGACCAAAATGTTCAAACTGCA
    TTAGAAAGCATTAATAATGGTGTGGATAATGGTGACGTTGATGATGCATTAACACAAGGT
    AAAGCAGCAATTGATGCTATTCAAGTAGATGCTACTGTTAAACCTAAAGCGAACCAAGCT
    ATTGAAGTTAAAGCAGAAGATACGAAAGAATCTATTGATCAAAGTGACCAGTTAACTGCT
    GAAGAAAAAACTGAAGCATTAGCAATGATTAAACAAATTACAGATCAAGCTAAACAAGGT
    ATTACTGATGCAACAACAACTGCTGAAGTTGAAAAAGCGAAAGCTCAAGGACTTGAAGCA
    TTTGATAACATTCAAATCGACTCAACAGAAAAACAAAAAGCTATCGAAGAATTAGAAACT
    GCACTAGACCAGATTGAAGCAGGTGTAAATGTCAACGCTGATGCTACAACTGAAGAAAAA
    GAAGCGTTTACGAATGCTTTAGAAGACATTTTATCAAAAGCAACTGAAGATATTTCTGAT
    CAAACTACAAATGCAGAAATCGCTACTGTCAAAAATAGTGCGCTTGAACAACTTAAAGCA
    CAACGTATTAATCCTGAAGTTAAGAAAAATGCTTTGGAAGCAATCAGAGAAGTGGTTAAC
    AAGCAAATAGAAATAATTAAAAATGCAGATGCAGATGCATCGGCGAAAGAAATTGCACGT
    ACGGATTTAGGTAGATATTTTGACAGATTTGCTGATAAATTAGATAAAACACAAACAAAT
    GCAGAAGTTGCTGAATTACAAAATGTAACGATACCTGCAATTGAAGCGATTGTGCCTCAG
    AATGATCCAGATGCTAATGATACTAATAATGGTATAGATAATAATGATGCAACAGCAAAT
    TCAAATGCTAATGCAACACCAGAAAATACTGGACAACCTAACGTATCTGAGACGACAGCT
    AATGGCAAAGCAGACGCTTCTCCGACAACACCAAATAATAGTGATGCGGCAACAGGTGAA
    ACAACAGCAACATCAGCAACTGATGATGCAAATGATAAACCGCAAGCTAATAACAATTCT
    AGTGTAGATGCATCAACAAACAGTCCGACAATGGATAACGATGTAACTAGTAAACCAGAA
    GTAGAATCAACTAATAACGGCACAACTGATAAACCTGTAACAGAAACTGATAATGCAACG
    CCAGCAGAAAGCACAACAAATAACAATAGTACAACTACAGCAACAAACGAGAATGCACCA
    ACAGGATCAACAGCAACGGCTCCAACAACTGCGAGCACAGAAGCAGCATCATCTGCTGAT
    AGCAAAGATAATGCATCTGTAAATGATTCAAAACAAAATGCTGAAGTAAATAATAGTGCT
    GAATCTCAATCAACTAATGACAAGGTTGCACAACCAAAATCTGAAAATAAAGCTAAGGCA
    GAAAAAGATGGTAGTGATTCAACAAATCAAAGTATGGTTGAATCAACAACTGAAACATTG
    CCTTCAGCAGACATAACAGAACCAAATGTACCTTCTAATACATCAAAAGATAAAGAAGAA
    AGTACGACAAATCAAACTGATGCAGGACAACTTAAGTCTGAAACTAACGTTGCATCTAAC
    GAAGCAGATAAATCGCCAAGCAAAGCTGATACTGAAGTTTCGAATAAACCATCAACATCT
    GCATCTTCTGAAGCAAAAGAAAAAATGACTTCAACTAATGTTAGCCAAAAAGATGATACG
    GCAACAGCAGATACTAATGATACGCAAAAATCAGTTGGTTCAGCTGCAAACAATAAAGCT
    ACGCAAAACGACGGTGCCAATGCATCTCCAGCTACAGTTTCAAATGGAAGCAATAGTGCT
    AATCAAGATATGCTTAATGTAACTAACACTGACGACCACCAAGCTAAGACAAAATCAGCT
    CAACAAGGAAAAGTTAATAAAGCTAAACAACAAGCTAAAACTTTACCAGATACTGGTATG
    TCACATAATGACGATTTACCATATGCTGAATTAGCTCTAGGTGCAGGTATGGCATTCTTA
    ATTAGAAGATTCACTAAGAAAGACCAACAAACTGAAGAATAA
    60
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    2160
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    2820
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    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3882

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2109
  • symbol: FmtB
  • description: truncated FmtB protein
  • length: 1293
  • theoretical pI: 4.12726
  • theoretical MW: 137185
  • GRAVY: -0.943852

Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 15.9)
  • TheSEED  :
    • FmtB (Mrp) protein involved in methicillin resistance and cell wall biosynthesis
    • truncated
  • PFAM:
    B_GA (CL0598) DUF1542; Domain of Unknown Function (DUF1542) (PF07564; HMM-score: 497.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014634
    • TAT(Tat/SPI): 0.002829
    • LIPO(Sec/SPII): 0.004331
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTQANSNIEAANSQNDVDQAKTTGENSIDQVTPTVNKKATARNEITAILNNKLQEIQATPDATDEEKQAADAEANTENGKANQAISAATTNAQVDEAKANAEAAINAVTPKVVKKQAAKDEIDQLQATQTNVINNDQNATTEEKEAAIQQLATAVTDAKNNITAATDDNGVDQAKDAGKNSIQSTQPATAVKSNAKNDVDQAVTTQNQAIDNTTGATTEEKNAAKDLVLKAKEKAYQDILNAQTTNDVTQIKDQAVADIQGITADTTIKDVAKDELATKANEQKALIAQTADATTEEKEQANQQVDAQLTQGNQNIENAQSIDDVNTAKDNAIQAIDPIQASTDVKTNARAELLTEMQNKITEILNNNETTNEEKGNDIGPVRAAYEEGLNNINAATTTGDVTTAKDTAVQKVQQLHANPVKKPAGKKELDQAAADKKTQIEQTPNASQQEINDAKQEVDTELNQAKTNVDQSSTNEYVDNAVKEGKAKINAVKTFSEYKKDALAKIEDAYNAKVNEADNSNASTSSEIAEAKQKLAELKQTADQNVNQATSKDDIEVQIHNDLDNINDYTIPTGKKESATTDLYAYADQKKNNISADTNATQDEKQQAIKQVDQNVQTALESINNGVDNGDVDDALTQGKAAIDAIQVDATVKPKANQAIEVKAEDTKESIDQSDQLTAEEKTEALAMIKQITDQAKQGITDATTTAEVEKAKAQGLEAFDNIQIDSTEKQKAIEELETALDQIEAGVNVNADATTEEKEAFTNALEDILSKATEDISDQTTNAEIATVKNSALEQLKAQRINPEVKKNALEAIREVVNKQIEIIKNADADASAKEIARTDLGRYFDRFADKLDKTQTNAEVAELQNVTIPAIEAIVPQNDPDANDTNNGIDNNDATANSNANATPENTGQPNVSETTANGKADASPTTPNNSDAATGETTATSATDDANDKPQANNNSSVDASTNSPTMDNDVTSKPEVESTNNGTTDKPVTETDNATPAESTTNNNSTTTATNENAPTGSTATAPTTASTEAASSADSKDNASVNDSKQNAEVNNSAESQSTNDKVAQPKSENKAKAEKDGSDSTNQSMVESTTETLPSADITEPNVPSNTSKDKEESTTNQTDAGQLKSETNVASNEADKSPSKADTEVSNKPSTSASSEAKEKMTSTNVSQKDDTATADTNDTQKSVGSAANNKATQNDGANASPATVSNGSNSANQDMLNVTNTDDHQAKTKSAQQGKVNKAKQQAKTLPDTGMSHNDDLPYAELALGAGMAFLIRRFTKKDQQTEE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]